RESEARCH PAPER
A small-scale survey of hantavirus in mammals from eastern Poland
 
More details
Hide details
1
Department of Zoonoses, Institute of Rural Health, Lublin, Poland
2
Department of Epidemiology, Warsaw Medical University, Warsaw, Poland
3
Department of Parasitology, National Veterinary Research Institute, Puławy, Poland
CORRESPONDING AUTHOR
Angelina Wójcik-Fatla   

Department of Zoonoses, Institute of Rural Health, Lublin, Poland
 
Ann Agric Environ Med. 2013;20(2):283–286
KEYWORDS
ABSTRACT
Samples of 30 dead small mammals each were collected on area ‘A’ located in eastern Poland which is exposed to flooding by the Vistula river, and on the area ‘B’, also located in eastern Poland but not exposed to flooding. Kidneys and livers of the mammals were examined by the PCR and nested PCR methods for the presence of hantavirus RNA. Out of 7 species of small mammals examined, the presence of hantaviruses was detected in 4 of them. Hantavirus prevalence was low in Apodemus agrarius (2.6%), the most numerous mammal species, whereas in the remaining 3 positive species (Microtus agrestis, Myodes glareolus, Sorex araneus) this was 12.5–100%. The presence of hantaviruses was detected only in the animals found on area ‘A’ exposed to flooding, and their prevalence was statistically greater compared to area ‘B’ not exposed to flooding (16.7% vs. 0%, p=0.0345). The overall positivity of the examined small mammals population from the areas ‘A’ and ‘B’ was 8.3%. The sequence analysis of the samples positive for hantavirus proved that the amplified products showed 77–86% homology with the L segment sequence of hantavirus Fusong-Mf-731 isolated from Microtus fortis in China. The presented study is the first to demonstrate the occurrence of hantavirus infection in small mammals from eastern Poland, and the first to demonstrate the significant relationship between flooding and the prevalence of hantaviruses in small mammals.
 
REFERENCES (31)
1.
Khaiboullina SF, Morzunov SP, St Jeor SC. Hantaviruses: molecular biology, evolution and pathogenesis. Curr Mol Med. 2005; 5: 773–790.
 
2.
Enria DA, Levis SC. Emerging viral zoonoses: hantavirus infections. Rev Sci Tech. 2004; 23(2): 595–611.
 
3.
Knap JP, Nowakowska A, Heyman P, Burzyński W, Rączka A, Dutkiewicz J, et al. Environmental and epidemiologic determinants of hantavirus infection (hemorrhagic fever with renal syndrome) in the Podkarpacie – first epidemic in Poland in 2007–2008 – and endemic incidences of diseases. Przegląd Medyczny Uniwersytetu Rzeszowskiego. 2009; 7 (2): 124–138 (in Polish).
 
4.
Nowakowska A, Heyman P, Knap JP, Burzyński W, Witas M. The first established focus of hantavirus infection in Poland, 2007. Ann Agric Environ Med. 2009; 16: 79–85.
 
5.
Stock I. Hantavirus infections. Med Monatsschr Pharm. 2008; 31: 127–133 (in German).
 
6.
Vapalahti O, Mustonen J, Lundkvist A, Henttonen H, Plyusnin A, Vaheri A. Hantavirus infections in Europe. Lancet Infect Dis. 2003; 3: 653–661.
 
7.
Jonsson CB, Figueiredo LT, Vapalahti O. A global perspective on hantavirus ecology, epidemiology, and disease. Clin Microbiol Rev. 2010; 23: 412–441.
 
8.
Song JW, Baek LJ, Song KJ, Skrok A, Markowski J, Bratosiewicz-Wasik J, et al. Characterization of Tula virus from common voles (Microtus arvalis) in Poland: evidence for geographic-specific phylogenetic clustering. Virus Genes. 2004; 29(2): 239–247.
 
9.
Krüger DH, Klempa B. From mice to men: the threat of hantavirus infections. Berl Munch Tierarztl Wochenschr. 2009; 122(11–12): 434–439.
 
10.
Krüger DH, Schönrich G, Klempa B. Human pathogenic hantaviruses and prevention of infection. Hum Vaccin. 2011; 7(6): 685–693.
 
11.
Piechotowski I, Brockmann SO, Schwarz C, Winter CH, Ranft U, Pfaff G. Emergence of hantavirus in South Germany: rodents, climate and human infections. Parasitol Res. 2008; 103 Suppl 1:S131-S137. Epub 2008 Nov 23.
 
12.
Klempa B. Hantaviruses and climate change. Clin Microbiol Infect. 2009; 15(6): 518–523.
 
13.
Schwarz AC, Ranft U, Piechotowski I, Childs JE, Brockmann SO. Risk factors for human infection with Puumala virus, southwestern Germany. Emerg Infect Dis. 2009; 15(7): 1032–1039.
 
14.
Stark K, Niedrig M, Biederbick W, Merkert H, Hacker J. Climate changes and emerging diseases. What new infectious diseases and health problem can be expected? Bundesgesundheitsblatt Gesundheitsforschung Gesundheitsschutz. 2009; 52(7): 699–714.
 
15.
Knap JP, Brzostek T, Rączka A, Burzyński W, Litarska U. A case of haemorrhagic fever with renal syndrom (HFRS). Pol Merk Lek. 2006; 21: 474–476 (in Polish).
 
16.
Knap JP, Nowakowska A, Dutkiewicz J, Zając V, Wójcik-Fatla A, Chmielewska-Badora J, Strupieniuk Z. Detection of antibodies against hantaviruses in forestry workers of the Roztoczański National Park and Puławy forest inspectorate (Lublin macroregion). Preliminary report. Med Ogólna 2010; 16: 201–216 (in Polish).
 
17.
Grygorczuk S, Pancewicz S, Zajkowska J, Kondrusik M, Świerzbińska R, Moniuszko A, Pawlak-Zalewska W. Detection of anti-hantavirus antibodies in forest workers in the north-east of Poland. Przegl Epidemiol. 2008; 62: 531–537 (in Polish).
 
18.
Arai S, Bennett SN, Sumibcay L, Cook JA, Song JW, Hope A, Parmenter C, Nerurkar VR, Yates TL, Yanagihara R. Short Report: Phylogenetically distinct hantaviruses in the masked shrew (Sorex cinereus) and dusky shrew (Sorex monticolus) in the United States. Am J Trop Hyg. 2008; 78: 348–351.
 
19.
Gresíková M, Rajcáni J, Sekeyová M, Brummer-Korvenkontio M, Kozuch O, Labuda M, Turek R, Weismann P, Nosek J, Lysý J. Haemorrhagic fever virus with renal syndrome in small rodents in Czechoslovakia. Acta Virol. 1984; 28(5): 416–21.
 
20.
Gresíková M, Sekeyová M, Brummer-Korvenkontio M, Kozuch O, Labuda M, Rajcáni J, Lysý J. Serological survey with the antigen of haemorrhagic fever virus with renal syndrome in small rodents in Slovakia. Acta Virol. 1986; 30(2): 158–60.
 
21.
LeDuc JW, Antoniades A, Siamopoulos K. Epidemiological investigations following an outbreak of hemorrhagic fever with renal syndrome in Greece. Am J Trop Med Hyg. 1986; 35(3): 654–659.
 
22.
Ferenczi E, Rácz G, Szekeres J, Balog K, Tóth E, Takács M, Csire M, Mezey I, Berencsi G, Faludi G. New data for the public health importance of hantaviruses in Hungary. Orv Hetil. 2003; 144(10): 467–474.
 
23.
Dietrich N, Pruden S, Ksiazek TG, Morzunov SP, Camp JW. A small-scale survey of hantavirus in mammals from Indiana. J Wildl Dis. 1997; 33(4): 818–822.
 
24.
Zhang YZ, Lin XD, Shi NF, Wang W, Liao XW, Guo WP, et al. Hantaviruses in small mammals and humans in the coastal region of Zhejiang Province, China. J Med Virol. 2010; 82: 987–995.
 
25.
Klein TA, Kang HJ, Gu SH, Moon S, Shim SH, Park YM, Lee SY, Kim HC, Chong ST, O’Guinn M, Lee JS, Turell MJ, Song JW. Hantaan virus surveillance targeting small mammals at Dagmar North Training Area, Gyeonggi Province, Republic of Korea, 2001–2005. J Vector Ecol. 2011; 36(2): 373–81. doi: 10.1111/j.1948–7134.2011.00178.x.
 
26.
Lim MY, Ryou J, Kim SY, Shin EH, Yoo YJ, Yun SM, Noh YT, Han MG, Ju YR. Seroprevalence of hantaviruses in small wild mammals trapped in South Korea from 2005 to 2010. J Vector Ecol. 2012; 37(1):97–101. doi: 10.1111/j.1948–7134.2012.00205.x.
 
27.
Niklasson B, LeDuc JW. Epidemiology of nephropathia epidemica in Sweden. J Infect Dis. 1987; 155(2): 269–276.
 
28.
Olsson GE, Hjertqvist M, Lundkvist A, Hörnfeldt B. Predicting high risk for human hantavirus infections, Sweden. Emerg Infect Dis. 2009; 15(1): 104–106.
 
29.
Evander M, Ahlm C. Milder winters in northern Scandinavia may contribute to larger outbreaks of haemorrhagic fever virus. Glob Health Action. 2009; 2. doi: 10.3402/gha.v2i0.2020.
 
30.
Verhagen R, Van der Groen G, Ivanov A, Van Rompaey J, Leirs H, Verheyen W. Occurrence and distribution of Hantavirus in wild living mammals in Belgium. Acta Virol. 1987; 31(1): 43–52.
 
31.
Lau CI, Smythe LD, Craig CB, Weinstein P. Climate change, flooding, urbanization and leptospirosis: fuelling the fire? Trans R Soc Trop Med Hyg. 2010; 104: 631-638.
 
eISSN:1898-2263
ISSN:1232-1966