Occupational exposure to airborne fungi in two Croatian sawmills and atopy in exposed workers
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Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia
Institute for Medical Research and Occupational Health, Zagreb, Croatia
Corresponding author
Maja Šegvić Klarić   

Department of Microbiology, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia
Ann Agric Environ Med. 2012;19(2):213-219
Airborne fungi were collected over a one year period at 2-month intervals at 2 sawmills in Croatia (SM 1 and SM 2) processing mainly beech wood and oak wood. A questionnaire concerning respiratory symptoms and skin prick test (SPT) with common inhalatory allergens and moulds Cladosporium herbarum, Alternaria alternata, Aspergillus niger, Penicillium notatum, and Rhizopus nigricans were performed in 96 workers from the same sawmills. Average concentrations of airborne fungi were 1,696-7,316 cfu/m3 in SM 1 and 1,706-4,819 cfu/m3 in SM 2, respectively. Health hazardous levels of airborne fungi (above 104/m3) were present only in SM 1. These levels were related to saw working sites and were season-dependent, i.e. present only during the summer. Penicillium (50-100%), Paecilomyces (43-100%) and Chrysonilia (33-100%) dominated among 17 fungal genera identified in both sawmills. Symptoms of rhinitis, asthma, and dry cough were most frequently recorded among analysed workers. SPT to moulds was negative in all tested workers, except one positive to R. nigricans, indicating that moderate airborne fungi levels found in the analysed sawmills were not related to IgE-mediated sensitization to moulds in exposed workers, even in atopics. Atopy was present among woodworkers in similar proportions to the general population of Croatia, suggesting that the wood-processing industry is not selective for atopic workers.
This work was financially supported by the Ministry of Science, Education and Sports of the Republic of Croatia (Grant Nos. 006-0061117-1242 and 022-0222411-2410).
Abdel Hameed AA, Khoder MI, Farag SA. Organic dust and gaseous contaminants at wood working shops. J Environ Monit. 2000; 2(1): 73-76.
Alwis KU, Mandryk J, Hocking AD. Exposure to biohazards in wood dust: bacteria, fungi, endotoxins, and (1›3)-beta-D-glucans. Appl Occup Environ Hyg. 1999; 14(9): 598-608.
Krysińska-Traczyk E, Skórska C, Cholewa G, Sitkowska G, Milanowski J, Dutkiewicz J. Exposure to airborne microorganisms in furniture factories. Ann Agric Environ Med. 2002; 9(1): 85-90.
Eduard W. Fungal spores: A critical review of the toxicological and epidemiological evidence as a basis for occupational exposure limit setting. Crit Rev Toxicol. 2009; 39(10): 799-864.
Opplinger A, Rusca S, Charriere N, Duc TV, Droz PO. Assessment of bioaerosols and inhalable dust exposure in Swiss sawmills. Ann Occup Hyg. 2005; 49(5): 385-391.
Madsen AM. Exposure to airborne microbial components in autumn and spring during work at Danish biofuel plants. Ann Occup Hyg. 2006; 50(8): 821-831.
Čavlović A, Lučić Beljo R, Ištvanić J. Exposure to wood dust in Croatian woodworking industry. Wood Res. 2009; 54(3): 109-116.
Lugauskas A, Krikštaponis A, Šveistyte L. Airborne fungi in industrial environments – potential agents of respiratory diseases. Ann Agric Environ Med. 2004; 11(1): 19-25.
Johansson SGO, Bieber T, Dahl R, Friedmann PS, Lanier BQ, Lockey RF et al. Revised nomenclature for allergy for global use: Report of the nomenclature review committee of the World allergy organization, October 2003. J Allergy Clin Immunol. 2004; 113(5): 832-836.
Monsó E, Magarolas R, Badorrey I, Radon K, Nowak D, Morera J. Occupational asthma in greenhouse flower and ornamental plant growers. Am J Respir Crit Care Med. 2002; 165(7): 954-960.
Zhang Y, Chen J, Chen Y, Dong J, Wei Q, Lou J. Environmental mycological study and allergic respiratory disease among tobacco processing workers. J Occup Health. 2005; 47(2): 181-187.
Douwes J, Thorne P, Pearce N, Heederik D. Bioaerosol health effects and esposure assessment: progress and prospects. Ann Occup Hyg. 2003; 47(3): 187-200.
Feller W. An Introduction to the probability theory and its application, 175. John Wiley and Sons Inc, New York, 1950.
de Hoog GS, Guarro J, Gene J, Figueras MJ. Atlas of clinical fungi. 2nd ed. Centraalbureau voor Schimmelcultures/Universitat Rovira i Virgili, Baard-Delft, 2000.
Pitt JI, Hocking AD. Fungi and food spoilage. Springer Science+Business Media LLC, New York, 2009.
Rylander R, Peterson Y, Donham KJ. Questionnaire evaluating organic dust exposure. Am J Ind Med. 1990; 17(1): 121-126.
Dreborg S, Frew A. Position paper: Allergen standardization and skin tests. Allergy 1993; 48(14 Suppl): 49-82.
Rimac D, Macan J, Varnai VM, Vučemilo M, Matković K, Prester L et al. Exposure to poultry dust and health effects in poultry workers: impact of mould and mite allergens. Int Arch Occup Environ Health. 2010; 83(1): 9-19.
Dutkiewicz J, Krysińska-Traczyk E, Prażmo Z, Skórska C, Sitkowska J. Exposure to airborne microorganisms in Polish sawmills. Ann Agric Environ Med. 2001; 8(1): 71-80.
Šegvić Klarić M, Pepeljnjak S. A year-round aeromycological study in Zagreb area, Croatia. Ann Agric Environ Med. 2006; 13(1): 55–64.
Simeray J, Mandin D, Chaumont JP. An aeromycological study of sawmills: effects of type of installation and timber on mycoflora and inhalation hazards for workers. Int Biodeter Biodegr. 1997; 40(1): 1 l-17.
Haas D, Galler H, Habib J, Melkes A, Schlacher R, Buzina W et al. Concentrations of viable airborne fungal spores and trichloroanisole in wine cellars. Int J Food Microbiol. 2010; 144(1): 126-132.
Oliveira AC, Peres CM, Correia Pires JM, Silva Pereira C, Vitorino S, Figueiredo Marques JJ et al. Cork stoppers industry: defining appropriate mould colonization. Microbiol Res. 2003; 158(2): 117–124.
San Romao MV. Biological diversity preservation as a challenge - the role of microbiological cork colonisation a short review. Ciencia Téc Vitiv. 2009; 24(2): 81-90.
Prester Lj, Macan J. Determination of Alt a 1 (Alternaria alternata) in poultry farms and a sawmill using ELISA. Med Mycol. 2010; 48(2): 298-302.
Jacobsen G, Schaumburg I, Sigsgaard T, Schlünssen V. Non-malignant respiratory diseases and occupational exposure to wood dust. Part I. Fresh wood and mixed wood industry. Ann Agric Environ Med. 2010; 17(1): 15-28.
Jacobsen G, Schaumburg I, Sigsgaard T, Schlünssen V. Non-malignant respiratory diseases and occupational exposure to wood dust. Part II. Dry wood industry. Ann Agric Environ Med. 2010; 17(1): 15-28.
Mandryk J, Udeni Alwis K, Hocking AD. Work-related symptoms and dose-response relationships for personal exposures and pulmonary function among woodworkers. Am J Ind Med. 1999; 35(5): 481-490.
Rusca S, Charriere N, Droz PO, Opplinger A. Effects of bioaerosol exposure on work-related symptoms among Swiss sawmill workers. Int Arch Occup Environ Health. 2008; 81(4): 415-421.
Dahlqvist M, Johard U, Alexandersson R, Bergström B, Ekholm U, Eklund A et al. Lung function and precipitating antibodies in low exposed wood trimmers in Sweden. Am J Ind Med. 1992; 21(4): 549-559.
Dennekamp M, Demers PA, Bartlett K, Davies HW, Teschke K. Endotoxin exposure among softwood lumber mill workers in the Canadian province of British Columbia. Ann Agric Environ Med. 1999; 6(2): 141-146.
Mandryk J, Udeni Alwis K, Hocking AD. Effects of personal exposures on pulmonary function and work-related symptoms among sawmill workers. Ann Occup Hyg. 2000; 44(4): 281-289.
Sabolić Pipinić I, Varnai VM, Beljo Lučić R, Čavlović A, Prester LJ, Orct T et al. Endotoxin exposure assessment in wood-processing industry: airborne versus settled dust levels. Arh Hig Rada Toksikol. 2010; 61(2): 161-166.
Macan J, Varnai VM, Maloča I, Kanceljak-Macan B. Increasing trend in atopy markers prevalence in a Croatian adult population between 1985 and 1999. Clin Exp Allergy. 2007; 37(12): 1756-1763.
Douwes J, McLean D, Slater T, Travier N, Cheng S, Pearce N: Pine dust, atopy and lung function: a cross-sectional study in sawmill workers. Eur Respir J 2006, 28, 791-798.
Schlünssen V, Schaumburg I, Heederik D, Taudorf E, Sigsgaard T. Indices of asthma among atopic and non-atopic woodworkers. Occup Environ Med. 2004; 61(6): 504-511.
Francuz B, Yera H, Geraut L, Bensefa-Colas L, Nghiem ZH, Choudat D. Occupational asthma induced by Chrysonilia sitophila in a worker exposed to coffee grounds. Clin Vaccine Immunol. 2010; 17(10): 1645-1646.
Rydjord B, Eduard W, Stensby B, Sandven P, Michaelsen TE, Wiker HG. Antibody response to long-term and high-dose mold-exposed sawmill workers. Scand J Immunol. 2007; 66(6): 711-718.
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