RESEARCH PAPER
Anti-inflammatory effect on genes expression after four days of Qigong training in peripheral mononuclear blood cells in healthy women
| 1 | Faculty of Medicine and Health Sciences, Jan Kochanowski University, Kielce, Poland |
| 2 | Faculty of Health Sciences, Medical College, Jagiellonian University, Kraków, Poland |
| 3 | Old Polish University, Kielce, Poland |
| 4 | Department of Sport Management, Faculty of Physical Education, Gdansk University of Physical Education and Sport, Gdańsk, Poland |
| 5 | Department of Physical Education, Faculty of Physical Education, Gdansk University of Physical Education and Sport, Gdańsk, Poland |
| 6 | Department of Histology and Embryology and Experimental Cytology Unit, Medical University, Lublin, Poland |
| 7 | Department of Life Sciences, Faculty of Physical Education, Gdansk University of Physical Education and Sport, Gdańsk, Poland |
CORRESPONDING AUTHOR
Małgorzata Żychowska
The Gdansk University of Physical Education and Sport, Department of Sport Management, Gdańsk, Poland, Górskiego, 1, 80-336 Gdańsk, Poland
The Gdansk University of Physical Education and Sport, Department of Sport Management, Gdańsk, Poland, Górskiego, 1, 80-336 Gdańsk, Poland
Ann Agric Environ Med. 2018;25(2):329–333
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Some studies have shown the influence of Qigong on gene expression in different cells, but there is little data associated with the influence of this kind of therapy on genes expression in pheripheral monocellucar blood cells.
Objective:
The aim of this study was to evaluate changes in the expression of genes associated with cellular stress response in peripheral mononuclear blood cells (PMBC) in healthy women.
Material and methods:
The experiment took place at the Japanese Martial Arts Centre “DOJO” in Stara Wieś, Poland, conducted over the course of a 4-day qigong training session. To evaluate the genes effect of this training, blood samples were taken before and after the training period. This experiment involved 20 healthy women (aged 56.2±9.01, body height 164.8±6.5 and mass 65.5±8.2). To determine the expression of HSF-1, HSPA1A, NF-kB, IL10 and CCL2 mRNA, 3 ml of venous blood was collected. The blood samples were placed in tubes allowing for separation (BD Vacutainer CPT TM) before and after the 4 days of qigong training. Isolated PMBC were used to determine gene expression using real-time qRT-PCR (quantitative reverse transcription polymerase chain reaction).
Results:
Significant decreases in NF-kB and CCL2 mRNA and increases in IL10, HSF1 and HSPA1A m-RNA were detected after 4 days of qigong training. The obtained findings suggest that qigong caused a reduction in the inflammatory and intensified anti-inflammatory gene expression, as well as a higher expression of HSF-1 and HSPA1A.
Conclusions:
The adaptive response to qigong training was similar to the adaptive response to physical activity and was detected through gene expression in PMBC. Furthermore, this kind of training is especially indicated for women because of their higher susceptibility to psychosocial stress when compared to men.
Some studies have shown the influence of Qigong on gene expression in different cells, but there is little data associated with the influence of this kind of therapy on genes expression in pheripheral monocellucar blood cells.
Objective:
The aim of this study was to evaluate changes in the expression of genes associated with cellular stress response in peripheral mononuclear blood cells (PMBC) in healthy women.
Material and methods:
The experiment took place at the Japanese Martial Arts Centre “DOJO” in Stara Wieś, Poland, conducted over the course of a 4-day qigong training session. To evaluate the genes effect of this training, blood samples were taken before and after the training period. This experiment involved 20 healthy women (aged 56.2±9.01, body height 164.8±6.5 and mass 65.5±8.2). To determine the expression of HSF-1, HSPA1A, NF-kB, IL10 and CCL2 mRNA, 3 ml of venous blood was collected. The blood samples were placed in tubes allowing for separation (BD Vacutainer CPT TM) before and after the 4 days of qigong training. Isolated PMBC were used to determine gene expression using real-time qRT-PCR (quantitative reverse transcription polymerase chain reaction).
Results:
Significant decreases in NF-kB and CCL2 mRNA and increases in IL10, HSF1 and HSPA1A m-RNA were detected after 4 days of qigong training. The obtained findings suggest that qigong caused a reduction in the inflammatory and intensified anti-inflammatory gene expression, as well as a higher expression of HSF-1 and HSPA1A.
Conclusions:
The adaptive response to qigong training was similar to the adaptive response to physical activity and was detected through gene expression in PMBC. Furthermore, this kind of training is especially indicated for women because of their higher susceptibility to psychosocial stress when compared to men.
REFERENCES (40)
1.
Astin JA, Shapiro SL, Eisenberg DM, Forys KL. Mind-body medicine: state of the science, implications for practice. J Am Board Fam Pract. 2003; 16(2): 131–47.
2.
Cacioppo JT, Cacioppo S. Social relationships and health: the toxic effects of perceived social isolation. Soc Pers Psychol Compass. 2014; 8(2): 58–72.
3.
Cohen L, Cole SW, Sood AK, Prinsloo S, Kirschbaum C, Arevalo JM, Jennings NB, Scott S, Vence L, Wei Q, Kentor D, Radvanyi L, Tannir N, Jonasch E, Tamboli P, Pisters L. Depressive symptoms and cortisol rhythmicity predict survival in patients with renal cell carcinoma: role of inflammatory signaling. PLoS One 2012; 7(8): 42324.
4.
Umberson D, Montez JK. Social relationships and health: a flashpoint for health policy. J Health Soc Behav. 2010; 51: S54–S66.
5.
Ernst E, Pittler MH, Wider B, Boddy K. Mind-body therapies: are the trial data getting stronger? Altern Ther Health Med. 2007; 13(5): 62–4.
6.
Jonas WB, Eisenberg D, Hufford D, Crawford C. The evolution of complementary and alternative medicine (CAM) in the USA over the last 20 years. Forsch Komplementmed. 2013; 20(1): 65–72.
7.
Loucks EB, Britton WB, Howe CJ, Eaton CB, Buka SL. Positive associations of dispositional mindfulness with cardiovascular health: the new england family study. Int J Behav Med. 2015; 22(4): 540–550.
8.
Kaliman P, Alvarez-Lopez MJ, Cosin-Tomas M, Rosenkranz MA, Lutz A, Davidson RJ. Rapid changes in histone deacetylases and inflammatory gene expression in expert meditators. Psychoneuroendocrinology 2014; 40: 96–107.
9.
Yan X, Shen H, Jiang H, Zhang C, Hu D, Wang J, Wu X. External Qi of Yan Xin Qigong differentially regulates the Akt and extracellular signal-regulated kinase pathways and is cytotoxic to cancer cells but not to normal cells. In J Biochem Cell Biol. 2006; 38: 2102–13.
10.
Yan X, Shen H, Jiang H, Hu D, Zhang Ch, Wang J, Wu X. External Qi of Yan Xin Qigong Induces Apoptosis and Inhibits Migration and Invasion of Estrogen-Independent Breast Cancer Cells Through Suppression of Akt/NF-κB Signaling. Cell Physiol Biochem. 2010; 25: 263–70.
11.
Połrola P, Góral Półrola J. In search a new methods. Qigong in stuttering therapy. Studia Medyczne 2013; 29 (3): 242–50.
12.
Loucks EB, Sullivan LM, D’Agostino Sr, RB, Larson MG, Berkman LF, Benjamin EJ. Social networks and inflammatory markers in the Framingham Heart Study. J Biosoc Sci. 2006; 38(6): 835–842.
13.
Yang YC, McClintock MK, Kozloski M, Li T. Social isolation and adult mortality: the role of chronic inflammation and sex differences. J Health Soc Behav. 2013; 54(2): 183–203.
14.
Dusek JA,Otu HH, Wohlhueter AL, Bhasin M, Zerbini LF, Joseph MG, Benson H, Libermann TA. Genomic Counter-Stress Changes Induced by the Relaxation Response. PLoS ONE 2008; 3(7): 2576. doi:10.1371/0002576.
15.
Bower JE, Irwin MR. Mind-body therapies and control of inflammatory biology: A descriptive review. Brain Behav Immun. 2016; 51: 1–11.
16.
Muehsama D, Lutgendorf S, Mills PJ, Rickhi B, Chevalier G, Batf N, Choprac D, Gurfeinh B. The embodied mind: A review on functional genomic and neurological correlates of mind-body therapies. Neurosc Biobehav Rev. 2017; 73: 165–181.
17.
Saatcioglu F. Regulation of gene expression by yoga, meditation and related practices: A review of recent studies, Asian J Psych. 2012; 6: 74–77.
18.
Li QZ, Li P, Garcia GE, Johnson RJ, Feng L. Genomic profiling of neutrophil transcripts in Asian Qigong practitioners: a pilot study in gene regulation by mind-body interaction. J Altern Complement Med. 2005; 11 (1): 29–39.
19.
Antoni MH, Lutgendorf SK, Blomberg B, Carver CS, Lechner S, Diaz A, Stagl J, Arevalo JM, Cole SW. Cognitive-behavioral stress management reverses anxiety-related leukocyte transcriptional dynamics. Biol Psychiatry 2012; 71(4): 366–72.
20.
Black DS, Cole SW, Irwin MR, Breen E, St Cyr NM, Nazarian N, Khalsa DS, Lavretsky H. Yogic meditation reverses NF-kappaB and IRF-related transcriptome dynamics in leukocytes of family dementia caregivers in a randomized controlled trial. Psychoneuroendocrinology 2013; 38 (3): 348–55.
21.
Creswell JD, Irwin MR, Burklund LJ, Lieberman MD, Arevalo JM, Ma J, Breen EC, Cole SW. Mindfulness-based stress reduction training reduces loneliness and pro-inflammatory gene expression in older adults: a small randomized controlled trial. Brain Behav Immun. 2012; 26(7): 1095–101.
22.
Bower JE, Greendale G, Crosswell AD, Garet D, Sternlieb B, Ganz PA, Irwin MR, Olmstead R, Arevalo J, Cole SW. Yoga reduces inflammatory signaling in fatigued breast cancer survivors: a randomized controlled trial. Psychoneuroendocrinology 2014; 43: 20–9.
23.
Kaliman P, Alvarez-Lopez MJ, Cosin-Tomas M, Rosenkranz MA, Lutz A, Davidson RJ. Rapid changes in histone deacetylases and inflammatory gene expression in expert meditators. Psychoneuroendocrinology 2014; 40: 96–10
24.
Sharma H, Datta P, Singh A, Sen S, Bhardwaj NK, Kochupillai V, Singh N. Gene expression profiling in practitioners of Sudarshan Kriya. J Psychosom Res. 2008; 64(2): 213–218.
25.
Xia M, Sui Z. Recent developments in CCR2 antagonists. Expert Opinion on Therapeutic Patents. 2009; 19(3): 295–303.
26.
Chomczynski P, Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Anal Biochem. 1987; 162: 156–9.
27.
Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative C(T) method. Nat Protoc. 2008; 3: 1101–8.
28.
Benson H, Greenwood MM, Klemchuk H. The relaxation response: psychophysiologic aspects and clinical applications. Int J Psychiatry Med. 1975; 6 (1–2): 87–98.
30.
Kajantie E, Phillips DI. The effects of sex and hormonal status on the physiological response to acute psychosocial stress. Psychoneuroendocrinology. 2006; 31: 151–78.
31.
Lundberg U. Stress hormones in health and illness: The roles of work and gender. Psychoneuroendocrinology. 2005; 30: 1017–21.
32.
Otte C, Hart S, Neylan TC, Marmar CR, Yaffe K, Mohr DC. A meta-analysis of cortisol response to challenge in human aging: Importance of gender. Psychoneuroendocrinology. 2005; 30: 80–91.
33.
Epel ES, Blackburn EH, Lin J, Dhabhar FS, Adler NE, et al. Accelerated telomere shortening in response to life stress. Proc Natl Acad Sci. USA 2004; 101: 17312–15.
34.
Morimoto RI, Tissieres A, Georgopoulos C. The biology of heat shock proteins and molecular chaperones. vol 26. Cold Spring Harbor Laboratory Press, 1994.
35.
Buttner P, Mosig S, Lechtermann A, Funke H, Mooren FC. Exercise affects the gene expression profiles of human white blood cells J Appl Physiol. 2007; 102: 26–36.
36.
Fisher G, et al. High Intensity Interval- vs Moderate Intensity- Training for Improving Cardiometabolic Health in Overweight or Obese Males: A Randomized Controlled Trial PloS one; 2015. 10:e0138853 doi:10.1371/journal.pone.0138853.
37.
Ziemann E, Zembron-Lacny A, Kasperska A, Antosiewicz J, Grzywacz T, Garsztka T, Laskowski R. Exercise training-induced changes in inflammatory mediators and heat shock proteins in young tennis players J Sports Sci Med. 2013; 12: 282–289.
38.
Yan X, Shen H, Jiang H, Zhang C, Hu D, Wang J, Wu X. External Qi of Yan Xin Qigong induces G2/M arrest and apoptosis of androgen-independent prostate cancer cells by inhibiting Akt and NF-kappa B pathways. Mol Cell Biochem. 2008 Mar; 310(1–2): 227–34.
39.
Yan X, et al. External Qi of Yan Xin Qigong induces cell death and gene expression alterations promoting apoptosis and inhibiting proliferation, migration and glucose metabolism in small-cell lung cancer cells. Mol Cell Biochem. 2012; 363(1–2): 245–55.
40.
Żychowska M, Kemeryte -Raubiene E, Gocentas A, Jascaniniene N, Chruściński G. Changes in leukocyte HSPA1A, HSPB1 mRNA in basketball players after plyometric training. BJPHA 2016; 8(1): 18–24.
RELATED ARTICLE
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.