Non-alcoholic beverages, unknown influence on cell proliferation – an in vitro study
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Department of Tissue Engineering, Nicolaus Copernicus University, Bydgoszcz, Poland
Tomasz Kloskowski   

Department of Tissue Engineering, Nicolaus Copernicus University, Bydgoszcz, Poland
Ann Agric Environ Med. 2014;21(1):113
Introduction and objective:
The aim of the presented study was to check differences between ‘Diet’ and ‘non-Diet’ soft drinks on cell proliferation.

Material and Methods:
Coca Cola and Pepsi Cola of different origin and their dietetic versions were examined at concentrations of 2% and 4%. Fructose and glucose as well as medium alone (control) were examined.

Cell number was higher in media supplemented with soft drinks, compared to control. Proliferation depended on the soft drink concentration and its origin, but not on sugar and calorific content.

An unknown factor is responsible for the increase in proliferation.

Brown CM, Dullo AG, Montani JP. Sugary drinks in the pathogenesis of obesity and cardiovascular diseases. Int J Obes (Lond). 2008; l6: 28–34.
Adamowicz J, Drewa T. Is there a link between soft drinks and erectile dysfunction? Cent Eur J Urol. 2011; 64(3): 140–3.
Cichocki M. Energy drinks and their contribution to current health concerns for children and adolescents. Przegl Lek. 2012; 69(10): 854–60.
Bhupathiraju SN, Pan A, Malik VS, Manson JE, Wilett WC, van Dam RM, et al. Caffeinated and caffeine – free beverages and risk of type 2 diabetes. Am J Clin Nutr. 2013; 97(1): 155–66.
Stanhope KL, Havel PJ. Endocrine and metabolic effects of consuming beverages sweetened with fructose, glucose, sucrose, or high-fructose corn syrup. Am J Clin Nutr. 2008; 88(6): 1733S-1737S.
Ferder L, Ferder MD, Inserra F. The role of high-fructose corn syrup in metabolic syndrome and hypertension. Curr Hypertens Rep. 2010; 12(2): 105–12.
Stanhope KL. Role of fructose-containing sugars in the epidemics of obesity and metabolic syndrome. Ann Rev Med. 2012; 63: 329–43.
Bremer AA, Stanhope KL, Graham JL, Cummings BP, Wang W, Saville BR, et al. Fructose-fed rhesus monkeys: a nonhuman primate model of insulin resistance, metabolic syndrome and type 2 diabetes. Clin Transl Sci. 2011; 4(4): 243–52.
Marcus Y, Shefer G, Sasson K, Kohen F, Limor R, Pappo O, et al. Angiotensin 1–7 as means to prevent the metabolic syndrome: Lesons from the fructose-fed rat model. Diabetes. 2012; in press.
De Moura RF, Ribeiro C, De Oliveira JA, Stevanato E, De Mello MA. Metabolic syndrome signs in Wistar rats submitted to different highfructose ingestion protocols. Br J Nutr. 2009; 101(8): 1178–1184.
Light HR, Tsanzi E, Gigliotti J, Morgan K, Tou JC. The type of caloric sweetener added to water influences weight gain, fat mass, and reproduction in growing Sprague-Dawley female rats. Exp Biol Med (Maywood). 2009; 234(6): 651–661.
Duffy A, Liew A, O’Sullivan J, Avalos G, Samali A, O’Brien T. Distinct effects of high-glucose conditions on endothelial cells of macrovascular and microvascular origins. Endothelium. 2006; 13(1): 9–16.
Deorosan B, Nauman EA. The role of glucose, serum, and threedimensional cell culture on the metabolism of bone marrow-derived mesenchymal stem cells. Stem Cells Int. 2011; 429187.