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RESEARCH PAPER
 
CC BY-NC-ND 3.0
 
 

Influence of iron on sperm motility and selected oxidative stress parameters in fertile males – a pilot study

 
1
Medical University of Silesia in Katowice, Department of Biochemistry, Zabrze, Poland
2
Medical University of Silesia in Katowice, Department of Microbiology and Immunology, Zabrze, Poland
3
Department of Molecular Biology and Translational Research, Institute of Rural Health, Lublin, Poland
4
University of Information Technology and Management, Department of Public Health, Rzeszów, Poland
Ann Agric Environ Med 2016;23(2):292–296
KEYWORDS:
ABSTRACT:
Introduction:
The presented study was designed to investigate the associations between iron level in seminal plasma and levels of the parameters of oxidative stress and antioxidant defence system and selected cytokines in the seminal plasma of fertile males.

Material and Methods:
The study population consisted of 65 fertile male volunteers from the southern region of Poland. Based on the medians of the levels of iron in seminal plasma, the study subjects were divided into 2 groups: with low and high levels of iron in seminal plasma (Fe-L and Fe-H).

Results:
The percentage of unprogressively motile sperm cells after 1 hour was significantly higher in the Fe-H group, compared to the Fe-L group. The activities of SOD and Mn-SOD and the level of MDA were significantly lower in the Fe-H group, compared to the Fe-L group. However, the value of TOC and OSI were significantly higher in the Fe-H group, compared to the Fe-L group. Median of IL-1β was significantly higher in the Fe-H group, compared to the Fe-L group, whereas medians of IL-2, IL-5, and GM-CSF were significantly lower in the Fe-H group.

Conclusions:
In fertile males, iron may negatively affect sperm motility and elevate oxidative stress intensity. Iron additionally modulates the levels of several cytokines in human semen.

CORRESPONDING AUTHOR:
Aleksandra Kasperczyk   
Medical University of Silesia in Katowice, Department of Biochemistry, Zabrze, Poland
 
REFERENCES:
1. Marzec-Wróblewska U, Kamiński P, Lakota P, Szymański M, Wasilow K, Ludwikowski G, Kuligowska-Prusińska M, Odrowąż-Sypniewska G, Stuczyński T, Michałkiewicz J. Zinc and iron concentration and SOD activity in human semen and seminal plasma. J Biol Trace Elem Res. 2011; 143(1): 167–177.
2. Morales C, Sylvester SR, Griswold MD. Transport of iron and transferrin synthesis by the seminiferous epithelium of the rat in vivo. Biol Reprod. 1987; 37(4): 995–1005.
3. Metzendorf C, Lind MI. Drosophila mitoferrin is essential for male fertility: evidence for a role of mitochondrial iron metabolism during spermatogenesis. BMC Dev Biol. 2010; 10: 68. doi: 10.1186/1471-213X-10-68.
4. Perera D, Pizzey A, Campbell A, Katz M, Poter J, Petrou M, Irvine DS, Chatterjee R Sperm DNA damage in potentially fertile homozygous β-thalassaemiapatients with iron overload. Hum Reprod. 2002; 17(7): 1820–1825.
5. Ayinde OC, Ogunnowo S, Ogedegbe RA. Influence of Vitamin C and Vitamin E on testicular zinc content and testicular toxicity in lead exposed albino rats. BMC Pharmacol Toxicol. 2012; 14: 13–17.
6. Huang YL, Tseng WC, Lin TH. In vitro effects of metal ions (Fe 2 +, Mn 2+ , Pb 2+ ) on sperm motility and lipid peroxidation in human semen. J Toxicol Environ Health 2001; 23(62): 259–267.
7. Sharma RK, Pasqualotto FF, Nelson DR, Thomas AJ Jr, Agarwal A. The reactive oxygen species-total antioxidant capacity score is a new measure of oxidative stress to predict male infertility. Hum Reprod. 1999; 14(11): 2801–2807.
8. Potts JM, Pasqualotto FF. Seminal oxidative stress in patients with chronic prostatitis. Andrologia. 2003; 35(5): 304–308.
9. Seshadri S, Bates M, Vince G, Jones DI. The role of cytokine expression in different subgroups of subfertile men. Am J Reprod Immunol. 2009; 62(5): 275–282.
10. WHO. Laboratory manual for the examination of human semen V ed. Cambridge University Press 2010.
11. Koster JF, Biemond P, Swaak AJ. Intracellular and extracellular sulphydryl levels in rheumatoid arthritis. Ann Rheum Dis. 1986; 45: 44–46.
12. Oyanagui Y. Reevaluation of assay methods and establishment of kit for superoxide dismutase activity. Anal Biochem 1984; 142: 290–296.
13. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979; 95: 351–358.
14. Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Bioch. 2005; 38: 1103–1111.
15. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Bioch. 2004; 37: 277–285.
16. Skandhan KP, Mazumdar BN, Sumangala B. Study into the iron content of seminal plasma in normal and infertile subjects. Urologia. 2012; 79(1): 54–57.
17. Aydemir B, Kiziler AR, Onaran I, Alici B, Ozkara H, Akyolcu MC. Impact of Cu and Fe concentrations on oxidative damage in male infertility. Biol Trace Elem Res. 2006; 112(3): 193–203.
18. Fraczek M, Sanocka D, Kamieniczna M, Kurpisz M. Proinflammatory cytokines as an intermediate factor enhancing lipid sperm membrane peroxidation in in vitro conditions. J Androl. 2008; 29(1): 85–92.
19. Martínez P, Proverbio F, Camejo MI. Sperm lipid peroxidation and pro-inflammatory cytokines. Asian J Androl. 2007; 9(1): 102–107.
20. Shen X, Lee K, König R. Effects of heavy metal ions on resting and antigen-activated CD4(+) T cells. Toxicology. 2001; 169(1): 67–80.
21. Politch JA, Tucker L, Bowman FP, Anderson DJ. Concentrations and significance of cytokines and other immunologic factors in semen of healthy fertile men. Hum Reprod. 2007; 22(11): 2928–2935.
22. Ota K, Jaiswal MK, Ramu S, Jeyendran R, Kwak-Kim J, Gilman-Sachs A, Beaman KD. Expression of a2 vacuolar ATPase in spermatozoa is associated with semen quality and chemokine-cytokine profiles in infertile men. PLoS One. 2013; 30;8(7):e70470. doi: 10.1371/journal.pone.0070470. Print 2013.
23. Rodríguez-Gil JE, Silvers G, Flores E, Jesús Palomo M, Ramírez A, Montserrat Rivera M, et al. Expression of the GM-CSF receptor in ovine spermatozoa: GM-CSF effect on sperm viability and motility of sperm subpopulations after the freezing-thawing process. Theriogenology. 2007; 67(8): 1359–1370.
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