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RESEARCH PAPER
 
 

Synthesis of Th17 cytokines in the culture of peripheral blood mononuclear cells stimulated with Borrelia burgdorferi sensu lato

Sambor Grygorczuk 1  ,  
Justyna Dunaj 1,  
 
1
Department of Infectious Diseases and Neuroinfections, Medical University, Białystok, Poland
Ann Agric Environ Med 2016;23(2):242–247
KEYWORDS:
ABSTRACT:
Introduction and objective:
Th17 lymphocytes and their cytokines, interleukin 17A (IL-17A), IL-17F and IL-22, participate in the response to extracellular bacteria and in the autoimmunity and may be engaged in the pathogenesis of Lyme borreliosis. Concentrations were measured of IL-17A, IL-17F and IL-22 in the supernatant of the peripheral blood mononuclear cells (PBMC) culture stimulated with Borrelia burgdorferi sensu lato (B. burgdorferi).

Material and Methods:
The study group consisted of 13 patients with early disseminated and late Lyme borreliosis and a control group of 7 healthy persons. PBMC cultures were stimulated for 48 hours with B. burgdorferi spirochetes of three pathogenic species: B. burgdorferi sensu stricto, B. afzelii or B. garinii, in the multiplicity of infection 10:1. Concentrations of Th17 cytokines IL-17A, IL-17F and IL-22, as well as Th2/immunoregulatory cytokine IL-10 were measured with ELISA assays.

Results:
Expression of IL-17A, IL-17F and IL-22 increased under stimulation, simultaneously with the increased IL-10 expression. Concentration of IL-17F tended to be lower in early neuroborreliosis than in late Lyme borreliosis and than in controls. B. afzelii elicited higher expression of IL-17A than the other two species.

Conclusions:
IL-17A, IL-17F and IL-22 are synthesized simultaneously by PBMC stimulated with B. burgdorferi. There is no antagonism between Th17 response and IL-10 expression. The role of Th17 cytokines seems to differ depending on the clinical stage of Lyme borreliosis and on the B. burgdorferi species.

CORRESPONDING AUTHOR:
Sambor Grygorczuk   
Department of Infectious Diseases and Neuroinfections, Medical University, Białystok, Poland
 
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