RESEARCH PAPER
Seroprevalence of Lyme disease and genospecies of Borrelia burgdorferi sensu lato in patients diagnosed with borreliosis in the Province of Warmia-Masuria in north-eastern Poland
 
More details
Hide details
1
Department of Medical Biology, University of Warmia and Mazury, Olsztyn, Poland
2
Provincial Sanitary-Epidemiological Station, Olsztyn, Poland
CORRESPONDING AUTHOR
Katarzyna Kubiak   

Department of Medical Biology, University of Warmia and Mazury, Olsztyn, Poland
 
Ann Agric Environ Med. 2012;19(2):203–207
KEYWORDS
ABSTRACT
Between 2009-2010, a group of 259 patients suspected of contracting Lyme disease were examined in the Provincial Sanitary-Epidemiological Station in Olsztyn for the presence of IgM and IgG against specific Borrelia burgdorferi sensu lato (s.l.) genospecies antigens by immunoblot. A total of 27.4% and 29.0% of the blood serum samples showed positive and uncertain results for IgM and IgG antibodies. The majority of positive and uncertain results were found in patients aged 30-40 years (30%) for IgM, and people aged 50 and over (35.8%) for IgG. Significantly more positive results for IgG were found in males (40.2%) then females (19.7%). In both groups, similar proportions of positive results for IgM anti-Borrelia were recorded (26.1% of females and 29% of males. In 71.5% of patients, IgM against flagellin protein (p41) of B. burgdorferi sensu stricto (s.s.) was found. For IgG, the most frequently detected antibodies were found against the p41 protein of B. burgdorferi s.s. (64.8%) and the recombinant variable surface antigens (VlsE) (49%). Among all the analysed antigens those of B. burgdorferi s.s. were the most frequent cause of immunological reaction, followed by antigens of B. afzelii and B. garinii. Reaction to antigens of B. spielmanii was rarely detected.
 
REFERENCES (38)
1.
Gern L. Borrelia burgdorferi sensu lato, the agent of Lyme borreriosis: life in the wilds. Parasite 2008; 15: 244-247.
 
2.
Burgdorfer G, Barbour AG, Hayes SF, Benach JL, Grunwaldt E, Davies JP. Lyme disease: a tick-born spirochetosis? Science 1982; 216: 1317.
 
3.
Nedelman RB, Wormser GP. Lyme borreliosis. Lancet 1998; 352: 557-565.
 
4.
van Dam AP, Kuiper H, Vos K, Widjojokusumo A, de Jongh BM, Spanjaard L, et al. Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations of Lyme borreliosis. Clin Infect Dis. 1993; 17: 708-717.
 
5.
Wang G, van Dam AP, Schwartz I, Dankert J. Molecular typing of Borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. Clin Microbiol Rev. 1999; 12: 633-653.
 
6.
Collares-Pereira M, Couceiro S, Franka I, Kurtenbach K, Schäfer SM, Vitorino L, et al. First isolation of Borrelia lusitaniae from a human patient. J Clin Microbiol. 2004; 42: 1313-1318.
 
7.
Diza E, Papa A, Vezyri E, Tsounis S, Milonas I, Antoniadis A. Borrelia valaisiana in cerebrospinal fluid. Emerg Infect Dis. 2004; 10: 1692-1693.
 
8.
Henneberg JP, Neubert U. Borrelia burgdorferi group: in-vitro antibiotic sensitivity. Orv Hetil. 2002; 143: 1195-1198.
 
9.
Rijpkema SG, Tazelaar DJ, Molkenboer MJ, Noordhoek GT, Plantiga G, Schouls LM, et al. Detection of Borrelia afzelii, Borrelia burgdorferi sensu stricto, Borrelia garinii and group VS116 by PCR in skin biopsies of patients with erythema migrans and acrodermatitis chronica atrophicans. Clin Microbiol Infect. 1997; 3: 109-116.
 
10.
Strle F, Picken RN, Cheng Y, Cimperman J, Maraspin V, Lotric-Furlan S, et al. Clinical findings for patients with Lyme borreliosis caused by Borrelia burgdorferi sensu lato with genotypic and phenotypic similarities to strain 25015. Clin Infect Dis. 1997; 25: 273-280.
 
11.
Wilske B, Fingerle V, Schulte-Spechtel U. Microbiological and serological diagnosis of Lyme borreliosis. FEMS Immunol Med Microbiol. 2007; 49: 13-21.
 
12.
Tylewska-Wierzbanowska S, Chmielewski T. Serological diagnosis of Lyme borreliosis – European guidelines. Post Mikrobiol. 2005; 44: 289-293 (in Polish).
 
13.
Wilske B, Zöller L, Brande V, Eiffert H, Göbel UB, Stanek G, et al. Quality standards for the microbiological diagnosis of infectious diseases. Lyme-Borreliose. http://pollux.mpk.med.uni-muen... (access: 2011.03.25).
 
14.
Pawińska A. Lyme borreliosis -microciological diagnostics and treatment. Standardy Med Pediatria. 2009; 6: 757-766 (in Polish).
 
15.
Aguero-Rosenfeld ME, Wang G, Schwartz I, Wormser GP. Diagnosis of Lyme borreliosis. Clin Microbiol Rev. 2005; 18: 484-509.
 
16.
Goettner G, Schulte-Spechtel U, Hillermann R, Liegl G, Wilske B, Fingerle V. Improvement of Lyme borreliosis serodiagnosis by a newly developed recombinant immunoglobulin G (IgG) and IgM line immunoblot assay and addition of VlsE and DbpA homologues. J Clin Microbiol. 2005; 43: 3602–3609.
 
17.
National Institute of Public Health – National Institute of Hygiene – Department of Epidemiology. Infectious diseases and poisonings in Poland in 1999-2010. http://www.pzh.gov.pl/oldpage/.... (access: 2011.03.01).
 
18.
Pancewicz SA, Januszkiewicz A, Hermanowska-Szpakowicz T. Detection of antibodies against Borrelia burgdorferi among inhabitants of north-eastern Poland. Przeg Epidemiol. 1996; 50: 375–381 (in Polish).
 
19.
Chmielewski T, Tylewska-Wierzbanowska S. Prevalence of Borrelia burgdorferi antibodies in health population in Poland. Przegl Epidemiol. 2002; 56: 33-38 (in Polish).
 
20.
Pancewicz SA, Zajkowska J, Kondrusik M, Snarska-Furła I, Świerzbińska R, Hermanowska-Szpakowicz T. Detectability of Borrelia burgdorferi antibodies among forestry workers of north-eastern Poland. Med Pr. 1998; 69: 253-260 (in Polish).
 
21.
Buczek A, Rudek A, Bartosik K, Szymańska J, Wójcik-Falta A. Seroepidemiological study of Lyme borreliosis among forestry workers in southern Poland. Ann Agric Environ Med. 2009; 19: 257-261.
 
22.
Dobracki W, Dobracka B, Paczosa W, Zięba J, Bereś P. Epidemiology of borreliosis in workers of the district forestry offices in Lower Silesia. Przegl Epidemiol. 2007; 61: 385–391 (in Polish).
 
23.
Dybowska, D, Kozielewicz D, Abdulgater A. Prevalence of borreliosis among forestry workers in Kujawsko-Pomorskie voivodeship. Przegl Epidemiol. 2007; 61: 67-71 (in Polish).
 
24.
Niścigorska J, Morańska I, Szych Z. Serological marker of Borrelia burgdorferi infection among forestry workers of West Pomerania region during a five year period. Adv Agric Sci. 2004; 9: 63–68.
 
25.
Cisak E, Chmielewska-Badora J, Zwoliński J, Wójcik-Fatla A, Zając V, Skórska C, et.al. Study on Lyme borreliosis focus in the Lublin region (Eastern Poland). Ann Agric Environ Med. 2008; 15: 327-332.
 
26.
Biesiada G, Czepiel J, Salamon D, Garlicki A, Dziubek A, Maziarz B, et al. Analysis of Borrelia burgdorferi genostrains among patients with Lyme disease. Przegl Lek. 2009; 66: 511-512 (in Polish).
 
27.
Tokarska-Rodak M, Fota-Markowska H, Kozioł-Montewka M, Śmiechowicz F, Modrzewska R. The detection of specific antibodies against B. burgdorferi s.s., B. afzelii, B. garinii, and B. spielmani antigens in patients with Lyme disease in eastern Poland. Now Med. 2010; 3: 84-87.
 
28.
Wojciechowska-Koszko I, Mączyńska I, Szych Z, Giedrys-Kalemba S. Serodiagnosis of borreliosis: indirect immunofluorescence assay, enzyme-linked immunosorbent assay and immunoblotting. Arch Immunol Ther Exp. 2011; 59: 69-77.
 
29.
Sicklinger M, Wienecke R, Neubert U. In vitro susceptibility testing for four antibiotics against Borrelia burgdorferi: a comparison of results for the three genospecies Borrelia afzelii, Borrelia garinii, and Borrelia burgdorferi sensu stricto. J Clin Microbiol. 2003; 41: 1791-1793.
 
30.
Stańczak J, Kubica-Biernat B, Kruminis-Łozowska W, Kur J. Detection of three genospecies of Borrelia burdgorferi sensu lato in Ixodes ricinus ticks collected in different regions of Poland. Int J Med Microbiol. 2000; 290: 559-566.
 
31.
Cisak E, Wójcik-Fatla A, Stojek MN, Chmielewska-Badora J, Zwoliński J, Buczek A, et al. Prevalence od Borrelia burgdorferi in Ixodes ricinus ticks from Lublin region (eastern Poland). Ann Agric Environ Med. 2006; 13: 301-306.
 
32.
Wodecka B, Sawczuk M. Occurence of pathogenic genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from north-western Poland. Wiad Parazytol. 2004; 50: 545-53 (in Polish).
 
33.
Derdáková M, Beati L, Pet’ko B, Stanko M, Fish D: Genetic variability within Borrelia burgdorferi sensu lato genospecies established by PCR-single-strand conformation polymorphism analysis of the rrfA-rrlB intergenic spacer in Ixodes ricinus ticks from the Czech Republic. Appl Environ Microbiol. 2003; 69: 509-516.
 
34.
Michel H, Wilske B, Hettche G, Göttner G, Heimerl C, Reischl U, et al. An ospA-polymerase chain reaction/restriction fragment length polymorphism-based method for sensitive detection and reliable differentiation of all European Borrelia burgdorferi sensu lato species and OspA types. Med Microbiol Immunol. 2004; 193: 219-226.
 
35.
Richter D, Schlee DB, Allgöwer R, Matuschka FR. Relationships of a novel Lyme disease spirochete, Borrelia spielmani sp. nov., with its hosts in central Europe. Appl Environ Microbiol. 2004; 70: 6414-6419.
 
36.
Jurczyszyn M, Wolk K. The present status of dormice (Myoxidae) in Poland. Nat Croat. 1998; 7: 11-18.
 
37.
Siński E, Pawełczyk A, Bajer A, Behnke JM. Abundance of wild rodents, ticks and environmental risk of Lyme borreliosis: longitudinal study in an area of Mazury Lakes district of Poland. Ann Agric Environ Med. 2006; 13: 295-300.
 
38.
Stańczak J, Racewicz M, Kubica-Biernat B, Kruminis-Łozowska W, Dabrowski J, Adamczyk A, et al. Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks (Acari, Ixodidae) in different Polish woodlands. Ann Agric Environ Med. 1999; 6: 127-132.
 
eISSN:1898-2263
ISSN:1232-1966