Pro-inflammatory cytokines in saliva of adolescents with dental caries disease
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Department of Biotechnology, Medical University, Bialystok, Poland
Department of Synthesis and Technology of Drugs, Medical University of Bialystok, Białystok, Poland
Maxillofacial and Plastic Surgery Clinic, Bialystok, Poland
Department of Public Health, University of Bialystok, Bialystok, Poland
Department of Developmental Period Medicine and Paediatric Nursing, Medical University, Bialystok, Poland
Ann Agric Environ Med 2012;19(4):711–716
Introduction and Objective:
Dental caries is an inflammatory disease with multifactorial etiology. The presented study was conducted to test the hypothesis that the elevation of salivary cytokines – interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor (TNF-a) is changed in dental caries patients. IL-6, IL-8 and TNF-a are particularly relevant to inflammation, one of the very first responses of the host to a pathological insult.

Material and Methods:
Whole saliva from 26 patients with dental caries, as well as 10 healthy persons, was investigated for the presence of IL-6, IL-8 and TNF-a by enzyme immunoassay – ELISA.

The results showed that an elevation of IL-6, IL-8 and TNF-a in unstimulated whole saliva in subjects with dental caries, compared with controls, increased and was statistically significant in all cases (p <0.05). The study also show a positive correlation between TNF-a and IL-8.

These data suggest links between the production of tumour necrosis factor (TNF-a), interleukin-6 (IL-6), interleukin-8 (IL-8) in saliva and dental caries disease.

Anna Bielawska   
Department of Biotechnology, Medical University, Bialystok, Poland
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