RESEARCH PAPER
Pro-inflammatory cytokines in saliva of adolescents with dental caries disease
 
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1
Department of Biotechnology, Medical University, Bialystok, Poland
2
Department of Synthesis and Technology of Drugs, Medical University of Bialystok, Białystok, Poland
3
Maxillofacial and Plastic Surgery Clinic, Bialystok, Poland
4
Department of Public Health, University of Bialystok, Bialystok, Poland
5
Department of Developmental Period Medicine and Paediatric Nursing, Medical University, Bialystok, Poland
 
Ann Agric Environ Med 2012;19(4):711–716
KEYWORDS:
ABSTRACT:
Introduction and Objective:
Dental caries is an inflammatory disease with multifactorial etiology. The presented study was conducted to test the hypothesis that the elevation of salivary cytokines – interleukin-6 (IL-6), interleukin-8 (IL-8) and tumour necrosis factor (TNF-a) is changed in dental caries patients. IL-6, IL-8 and TNF-a are particularly relevant to inflammation, one of the very first responses of the host to a pathological insult.

Material and Methods:
Whole saliva from 26 patients with dental caries, as well as 10 healthy persons, was investigated for the presence of IL-6, IL-8 and TNF-a by enzyme immunoassay – ELISA.

Results:
The results showed that an elevation of IL-6, IL-8 and TNF-a in unstimulated whole saliva in subjects with dental caries, compared with controls, increased and was statistically significant in all cases (p <0.05). The study also show a positive correlation between TNF-a and IL-8.

Conclusions:
These data suggest links between the production of tumour necrosis factor (TNF-a), interleukin-6 (IL-6), interleukin-8 (IL-8) in saliva and dental caries disease.

CORRESPONDING AUTHOR:
Anna Bielawska   
Department of Biotechnology, Medical University, Bialystok, Poland
 
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