Prevalence of tick-borne pathogens in an urban park in Rome, Italy
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Department of Infectious, Parasitic and Immune­Mediated Diseases, Istituto Superiore di Sanità, Rome, Italy
Regional Park of Bracciano and Martignano, Rome, Italy
Ann Agric Environ Med. 2014;21(4):723–727
Limited information is available about the presence of tick-borne pathogens in urban parks in Italy. To fill this gap, ticks were collected in a public park in Rome over a 1-year period and screened by molecular methods for tick-borne pathogens.

Results and conclusion:
The most abundant tick species were Rhipicephalus turanicus and Ixodes ricinus. The predominant pathogens detected were Borrelia. burgdorferi sensu lato (36%), Rickettsia spp. (36%), and Coxiella burnetii (22%). Among less frequently detected pathogens, Babesia microti was detected for the first time in Italy, with a prevalence of 4%. Neither Bartonella spp. nor Francisella tularensis were detected. With regard to co-infections, the most frequent double and triple infections involved Rickettsia spp., B. burgdorferi sl., and C. burnetii.. A positive correlation was detected between pathogens and I. ricinus. Further studies are needed in order to assess risk associated with tick-borne pathogens in urban areas.

Parola P, Raoult D. Ticks and tick-borne bacterial diseases in humans: an emerging infectious threat. Clin Infect Dis. 2001; 32: 897–928.
Comer JA, Paddock CD, Childs JE Urban zoonoses caused by Bartonella, Coxiella, Ehrlichia, and Rickettsia species. Vector Borne Zoonotic Dis. 2001; 1: 91–118.
Stańczak J, Gabre RM, Kruminis-Łozowska W, Racewicz M, KubicaBiernat B: Ixodes ricinus as a vector of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in urban and suburban forests. Ann Agric Environ Med. 2004; 11: 109–114.
Wielinga PR, Gaasenbeek C, Fonville M, de Boer A, de Vries A, Dimmers W, et al. Longitudinal analysis of tick densities and Borrelia, Anaplasma, and Ehrlichia infections of Ixodes ricinus ticks in different habitat areas in The Netherlands. Appl Environ Microbiol. 2006; 72: 7594–7601.
Ciceroni L, Ciarrocchi S, Simeoni J. Antigenic and genomic analysis of a Borrelia burgdorferi sensu stricto strain isolated from Ixodes ricinus ticks in Alto Adige-South Tyrol, Italy. Eur J Epidemiol. 1998; 14: 511–517.
Cinco M, Padovan D, Murgia R, Frusteri L, Maroli M, van de Pol I, et al. Prevalence of Borrelia burgdorferi infection in Ixodes ricinus in central Italy. Eur J Clin Microbiol Infect Dis. 1998; 17: 134–135.
Favia G, Cancrini G, Carfi A, Grazioli D, Lillini E, Iori A. Molecular identification of Borrelia valaisiana and HGE-like Ehrlichia in Ixodes ricinus ticks sampled in north-eastern Italy: first report in Veneto region. Parassitologia. 2001; 43: 143–146.
Piccolin G, Benedetti G, Doglioni C, Lorenzato C, Mancuso S, Papa N, et al. A study of the presence of B. burgdorferi, Anaplasma (previously Ehrlichia) phagocytophilum, Rickettsia, and Babesia in Ixodes ricinus collected within the territory of Belluno, Italy. Vector Borne Zoonotic Dis. 2006; 6: 24–31.
Corrain R, Drigo M, Fenati M, Menandro ML, Mondin A, Pasotto D, et al. Study on ticks and tick-borne zoonoses in public parks in Italy. Zoonoses Public Health. 2012; 6: 468–476.
Floris R, Yurtman AN, Margoni EF, Mignozzi K, Boemo B, Altobelli A, et al. Detection and identification of Rickettsia species in the northeast of Italy. Vector Borne Zoonotic Dis. 2008; 8: 777–782.
Di Luca M, Toma L, Bianchi R, Quarchioni E, Marini L, Mancini F, et al. Seasonal dynamics of tick species in a urban park of Rome. Ticks Tick-borne Dis. 2013; 4: 513–517.
Manilla G. Acari Ixodida. In: Calderini (ed). Fauna d’Italia. Bologna 1998. vol 36.
Regnery RL, Spruill CL, Plikaytis BD. Genotypic identification of rickettsiae and estimation of intraspecies sequence divergence for portions of two rickettsial genes. J Bacteriol 1991; 173:1576–1589.
Lee JH, Park HS, Jang WJ, Koh SE, Kim JM, Shim SK, et al. Differentiation of rickettsiae by groEL gene analysis. J Clin Microbiol. 2003; 41:2952–2960.
Parola P, Roux V, Camicas JL, Baradji I, Brouqui P, Raoult D. Detection of Ehrlichiae in African ticks by polymerase chain reaction. Trans R Soc Trop Med Hyg. 2000; 94: 707–708.
Persing DH, Mathiesen D, Marshall WF, Telford SR, Spielman A, Thomford JW, et al. Detection of Babesia microti by polymerase chain reaction. J Clin Microbiol. 1992; 30: 2097–2103.
Pietilä J, He Q, Oksi J, Viljanen MK. Rapid differentiation of Borrelia garinii from Borrelia afzelii and Borrelia burgdorferi sensu stricto by LightCycler fluorescence melting curve analysis of a PCR product of the recA gene. J Clin Microbiol. 2000; 38: 2756–2759.
Ciervo A, Ciceroni L. Rapid detection and differentiation of Bartonella spp. by a single-run real-time PCR. Mol Cell Probes 2004; 18: 307–312.
Klee SR, Tyczka J, Ellerbrok H, Franz T, Linke S, Baljer G, et al. Highly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii. BMC Microbiol. 2006; 6: 2.
Kugeler KJ, Pappert R, Zhou Y, Petersen JM. Real-time PCR for Francisella tularensis types A and B. Emerg Infect Dis. 2006; 12: 1799–1801.
Dietrich F, Schmidgen T, Maggi RG, Richter D, Matuschka FR, Vonthein R, et al. Prevalence of Bartonella henselae and Borrelia burgdorferi sensu lato DNA in Ixodes ricinus ticks in Europe. Appl Environ Microbiol. 2010; 76: 1395–1398.
Stanczak J The occurrence of Spotted Fever Group (SFG) Rickettsiae in Ixodes ricinus ticks (Acari: Ixodidae) in northern Poland. Ann N Y Acad Sci. 2006; 1078: 512–514.
Reis C, Cote M, Paul RE, Bonnet S. Questing ticks in suburban forest are infected by at least six tick-borne pathogens. Vector Borne Zoonotic Dis. 2010; 11: 907- 916.
Parola P, Raoult D. Tick-borne bacterial diseases emerging in Europe. Clin Microbiol Infect. 2001; 7: 80–83.
Wielinga PR, Fonville M, Sprong H, Gaasenbeek C, Borgsteede F, Giessen JW. Persistent Detection of Babesia EU1 and Babesia microti in Ixodes ricinus in The Netherlands During a 5-Year Surveillance: 2003–2007. Vector Borne Zoonotic Dis. 2008; 9: 119–122.
Floris R, Altobelli A, Boemo B, Mignozzi K, Cinco M. First detection of TBE virus sequences in Ixodes ricinus from Friuli Venezia Giulia (Italy). New Microbiol. 2006; 29: 147–150.
Ciceroni L, Pinto A, Ciarrocchi S, Ciervo A. Current knowledge of rickettsial diseases in Italy. Ann NY Acad Sci. 2006; 1078: 143–149.
Toledo A, Jado I, Olmeda AS, Casado-Nistal MA, Gil H, Escudero R, et al. Detection of Coxiella burnetii in ticks collected from Central Spain. Vector Borne Zoonotic Dis. 2009; 9: 465–468.
Angelakis E, Billeter SA, Breitschwerdt EB, Chomel BB, Raoult D. Potential for tick-borne bartonelloses. Emerg Infect Dis. 2010: 16; 385–391.
Iori A, Gabrielli S, Calderini P, Moretti A, Pietrobelli M, Tampieri MP, et al. Tick reservoirs for piroplasms in central and northern Italy. Vet Parasitol. 2010; 170: 291–296.
Pennisi MG, Alongi A, Agnone A, Vitale F, Reale S, Torina A. Cats as reservoir of Babesia microti. Parassitologia. 2007; 49: 100.
Gray J, Zintl A, Hildebrandt A, Hunfeld KP, Weiss L: Zoonotic babesiosis: overview of the disease and novel aspects of pathogen identity. Ticks Tick-borne Dis. 2010; 1: 3–10.