RESEARCH PAPER
Isolation of Listeria monocytogenes from milks used for Iranian traditional cheese in Lighvan cheese factories
1
Department of Food Hygiene and Aquatic, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
2
Department of Epidemiology, Pasteur Institute of Iran, Tehran, Iran
3
Department of Epidemiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Corresponding author
Saber Esmaeili
No. 69, Pasteur Ave., Department of Epidemiology, Pasteur Institute of Iran, Postal Code: 1316943551, Tehran, Iran
No. 69, Pasteur Ave., Department of Epidemiology, Pasteur Institute of Iran, Postal Code: 1316943551, Tehran, Iran
Ann Agric Environ Med. 2014;21(4):728–729
KEYWORDS
ABSTRACT
Traditional Lighvan cheese is a semi-hard cheese which has a popular market in Iran and neighboring countries. The aim of this study was evaluating the contamination of milks used for Lighvan cheese making with Listeria monocytogenes. Raw milk samples were randomly collected from different cheese producing factories (sampling carried out from large milk tanks used cheese making in factories). Isolation of L. monocytogenes was performed according to ISO 11290 and biochemical tests were done to identify and confirm L. monocytogenes. 9 samples (50%) of the 18 collected samples from milk tanks in Lighvan cheese producing factories were contaminated with L. monocytogenes. The concentration of L. monocytogenes in all 9 positive samples was 40 CFU/ml. This study is the first report of L. monocytogenes contamination in raw milks used for Lighvan cheese production in Iran. Regarding the fact that these cheeses are produced from raw milk and no heating process is performed on them its milk contamination can be a potential risk for consumers.
REFERENCES (15)
1.
Adzitey F, Huda N. Listeria monocytogenes in foods: Incidences and possible control measures. Afr J Microbiol Res. 2010; 4: 2848–2855.
2.
Swaminathan B, Gerner-Smidt P. The epidemiology of human listeriosis. Microbes Infect. 2007; 9: 1236–1243.
3.
Amagliani G, Brandi G, Omiccioli E, Casiere A, Bruce I, Magnani M. Direct detection of Listeria monocytogenes from milk by magnetic based DNA isolation and Pcr. Food Microbiol. 2004; 21: 597–603.
4.
Lundén J, Tolvanen R, Korkeala H. Human listeriosis outbreaks linked to dairy products in Europe. J Dairy Sci. 2004; 87: 6–12.
5.
Jalali M, Abedi D. Prevalence of Listeria species in food products in Isfahan, Iran. Int J Food Microbiol. 2008; 122: 336–340.
6.
Mirzaei H. Microbiological changes in Lighvan cheese throughout its manufacture and ripening. A Afr J Microbiol Res. 2011; 5: 1609–1614.
7.
Abdi R, Sheikh-Zeinoddin M, Soleimanian-Zad S. Identification of lactic acid bacteria isolated from traditional Iranian Lighvan cheese. Pakistan Journal of Biological Sciences. 2006; 9: 99–103.
8.
Gasanov U, Hughes D, Hansbro PM. Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review. Fems Microbiol Rev. 2005; 29: 851–875.
9.
Jami S, Jamshidi A, Khanzadi S. The presence of Listeria monocytogenes in raw milk samples in Mashhad, Iran. Iran J Veterinary Re. 2011; 11: 363–367, 393.
10.
Mahmoodi MM. Occurrence of Listeria monocytogenes in Raw Milk and Dairy Products in Noorabad, Iran. J Anim Vet Adv. 2010; 9: 16–19.
11.
Moshtaghi H, Mohamadpour AA. Incidence of Listeria spp. in raw milk in Shahrekord, Iran. Foodborne Pathog Dis. 2007; 4: 107–110.
12.
Vitas A, Garcia-Jalon V. Occurrence of Listeria monocytogenes in fresh and processed foods in Navarra (Spain). Int J Food Microbiol. 2004; 90: 349–356.
13.
Okutani A, Okada Y, Yamamoto S, Igimi S. Overview of Listeria monocytogenes contamination in Japan. Int J Food Microbiol. 2004; 93: 131–140.
14.
Bemrah N, Sanaa M, Cassin M, Griffiths M, Cerf O. Quantitative risk assessment of human listeriosis from consumption of soft cheese made from raw milk. Prev Vet Med. 1998; 37: 129–145.
15.
Lavasani ARS, Ehsani M, Mirdamadi S, Mousavi S. Changes in physicochemical and organoleptic properties of traditional Iranian cheese Lighvan during ripening. Int J Dairy Technol. 2012; 65: 64–70.
Share
RELATED ARTICLE
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.