RESEARCH PAPER
What is the percentage of pathogenic borreliae in spirochaetal findings of mosquito larvae?
| 1 | Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, CZ-61137 Brno, Czech Republic. |
Ann Agric Environ Med. 2009;16(2):273–276
KEYWORDS
ABSTRACT
In this study, larvae (1,179 ex.) of mosquito genera Culex were examined for the presence of spirochaetes by Dark Field Microscopy (DFM) at the locality of Blansko (Czech Republic) in of 2004-2008. DFM spirochaete positive samples (25.4 %) were investigated by nested PCR; only 4 samples were positive for the presence of Borrelia burgdorferi sensu lato, which is 0.3 % of the total examined samples. We can conclude that only a low percentage of pathogenic borreliae are presented in mosquito larvae, while the spirochaete of undefined genera infect larvae in high amounts.
REFERENCES (26)
1.
Bašta J, Hulínská D, Plch J, Daniel M: Application of the single–step polymerase chain reaction for detection of Borrelia burgdorferi sensu lato and their genome species in Ixodes ricinus ticks. Epidemiol Microbiol Imunol 1999, 48, 167-170.
2.
Čapková L, Žákovská A, Knoz J, Dendis M, Šerý O: Further spirochaetes findings in Culex sp., Aedes sp. mosquitoes and in Ixodes ricinus ticks. Biologia (Bratisl) 2002, 57, 389-394.
3.
Doby JM, Anderson JF, Couatarmanac’h A, Magnarelli LA, Martin A: Lyme disease in Canada with possible transmission by an insect. Zentralbl Bacteriol Microbiol Hyg 1987, 263, 488-490.
4.
Gern L, Burgdorfer W, Aeschlimann A, Krampitz HE: The ecology of Lyme borreliosis in Europe. In: Weber K, Burgdorfer W (Eds): Aspect of Lyme Borreliosis, 59-69. Springer, Berlin 1993.
5.
Halouzka J: Borreliae in Aedes vexans and hibernating Culex pipiens molestus mosquitoes. Biologia (Bratisl) 1993, 48, 123-124 (in Slovak).
6.
Halouzka J, Postic D, Hubálek Z: Isolation of the spirochaete Borrelia afzelii from the mosquito Aedes vexans in the Czech Republic. Med Vet Entomol 1998, 12, 103-105.
7.
Halouzka J, Wilske B, Stünzner D, Sanogo YO, Hubálek Z: Isolation of Borrelia afzelii from overwintering Culex pipiens biotype molestus mosquitoes. Infection 1999, 27, 275-277.
8.
Hubálek Z, Halouzka J: Prevalence rates of Borrelia burgdorferi sensu lato in host-seeking Ixodes ricinus ticks in Europe. Parasitol Res 1998, 84, 167-172.
9.
Hubálek Z, Halouzka J, Juřicová Z: Investigation of haematopha-gous arthropods for borreliae – summarized data 1988–1996. Folia Parasitol 1998, 45, 67-72.
10.
Janovská D: Epidemiologická situace lymeské borreliózy v ČR v r. 2000–2002, Seminář Lymeská borrelióza a ehrlichióza/rozlišeni zdravotníiho rizika, Praha, in Czech 2002.
11.
Kahl O, Gern L, Gray JS, Guy EC, Jongejan F, Kirstein F, Kurtenbach K, Rijpkema SGT, Stanek G: Detection of Borrelia burgdorferi sensu lato in ticks: immunofluorescence assay versus polymerase chain reaction. Zentralbl Bacteriol Microbiol Hyg 1998, 287, 205-210.
12.
Kosik-Bogacka D, Bukowska K, Kuźna-Grygiel W: Detection of Borrelia burgdorferi sensu lato in mosquitoes (Culicidae) in recreation areas of the city of Szczecin. Ann Agric Environ Med 2002, 9, 55-57.
13.
Kosik-Bogacka D, Kuźna-Grygiel W, Bukowska K: The prevalence of spirochaete Borrelia burgdorferi sensu lato in ticks Ixodes ricinus and mosquitoes Aëdes spp. within a selected recreational area in the city of Szczecin. Ann Agric Environ Med 2004, 11, 105-108.
14.
Kosik-Bogacka D, Kuźna-Grygiel W, Górnik K: Borrelia burgdorferi sensu lato infection in mosquitoes from Szczecin area. Folia Biol 2006, 54(1-2), 55-59.
15.
Kosik-Bogacka D, Kuźna-Grygiel W, Jamborowska M: Ticks and mosquitoes as a vectors of Borrelia burgdorferi s.l. in the forested areas of Szczecin. Folia Biol 2007, 55(3-4), 143-146.
16.
Kubica-Biernat B, Stańczak J, Racewicz M, Kruminis-Lozowska W: Detection of etiological agent of lyme borreliosis in native mosquitoe (Diptera: Culicidae) population. Wiad Parazytol 1998, 44, 756-757.
17.
Luger SW: Lyme disease transmitted by a biting fly. N Eng J Med 1990, 322, 1752.
18.
Magnarelli LA, Freier JE, Anderson JF: Experimental infections of mosquitoes with Borrelia burgdorferi, the etiologic agent of Lyme disease. J Infect Dis 1987, 156, 694-695.
19.
Matuschka FR, Richter D: Mosquitoes and soft ticks cannot transmit lyme disease spirochetes. Parasitol Res 2002, 88, 283-284.
20.
Picken MM, Picken RN, Han D, Cheng Y, Strle F: Single-tube nested polymerase chain reaction assay based on Flagellin gene sequences for detection of Borrelia burgdorferi sensu lato. Eur J Clin Microbiol Infect Dis 1996, 15, 489-498.
21.
Sanogo YO, Halouzka J, Hubálek Z, Němec M: Detection of spirochetes in, and isolation from, Culicine mosquitoes. Folia Parasitol 2000, 47, 79-80.
22.
Stańczak J, Kubica/Biernat B, Racewicz M, Kruminis-Lozowska W: Occurrence of Borrelia spirochetes in haematophagous insects (Diptera, Siphonaptera, Anoplura). In: Programme and Abstract. IX SOVE European Meeting, 49. 4–7 Sept.; Prague, Czech Republic 1995.
23.
Žákovská A, Čapková L, Šerý O, Halouzka J, Dendis M: Isolation of Borrelia afzelii from overwintering Culex pipiens biotype molestus mosquitoes. Ann Agric Environ Med 2006, 13, 345-348.
24.
Žákovská A, Dendis M, Pejchalová K: Spirochaetes in Aedes species, Culex pipiens pipiens larvae and hibernating Culex pipiens molestus mosquitoes detected with darkfield microscopy (DFM) and polymerase chain reaction (PCR) methods. Biologia (Bratisl) 2000, 55, 667-670.
25.
Žákovská A, Jörkova M, Šerý O, Dendis M: Spirochaetes in Culex (C.) pipiens s.l. larvae. Biologia (Bratisl) 2004, 59, 283-287.
26.
Žákovská A, Nejedlá P, Holíková A, Dendis M: Positive findings of Borrelia burgdorferi in Culex (Culex) pipiens pipiens larvae in the surrounding of Brno city determined by the PCR method. Ann Agric Environ Med 2002, 9, 257-259.
RELATED ARTICLE
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.