Department of Animal Physiology and Immunology, Institute of Experimental Biology, Faculty of Science, Masaryk University, Kotlarska 2, CZ-61137 Brno, Czech Republic.
In this study, larvae (1,179 ex.) of mosquito genera Culex were examined for the presence of spirochaetes by Dark Field Microscopy (DFM) at the locality of Blansko (Czech Republic) in of 2004-2008. DFM spirochaete positive samples (25.4 %) were investigated by nested PCR; only 4 samples were positive for the presence of Borrelia burgdorferi sensu lato, which is 0.3 % of the total examined samples. We can conclude that only a low percentage of pathogenic borreliae are presented in mosquito larvae, while the spirochaete of undefined genera infect larvae in high amounts.
REFERENCES(26)
1.
Bašta J, Hulínská D, Plch J, Daniel M: Application of the single–step polymerase chain reaction for detection of Borrelia burgdorferi sensu lato and their genome species in Ixodes ricinus ticks. Epidemiol Microbiol Imunol 1999, 48, 167-170.
Čapková L, Žákovská A, Knoz J, Dendis M, Šerý O: Further spirochaetes findings in Culex sp., Aedes sp. mosquitoes and in Ixodes ricinus ticks. Biologia (Bratisl) 2002, 57, 389-394.
Doby JM, Anderson JF, Couatarmanac’h A, Magnarelli LA, Martin A: Lyme disease in Canada with possible transmission by an insect. Zentralbl Bacteriol Microbiol Hyg 1987, 263, 488-490.
Gern L, Burgdorfer W, Aeschlimann A, Krampitz HE: The ecology of Lyme borreliosis in Europe. In: Weber K, Burgdorfer W (Eds): Aspect of Lyme Borreliosis, 59-69. Springer, Berlin 1993.
Halouzka J, Postic D, Hubálek Z: Isolation of the spirochaete Borrelia afzelii from the mosquito Aedes vexans in the Czech Republic. Med Vet Entomol 1998, 12, 103-105.
Hubálek Z, Halouzka J: Prevalence rates of Borrelia burgdorferi sensu lato in host-seeking Ixodes ricinus ticks in Europe. Parasitol Res 1998, 84, 167-172.
Janovská D: Epidemiologická situace lymeské borreliózy v ČR v r. 2000–2002, Seminář Lymeská borrelióza a ehrlichióza/rozlišeni zdravotníiho rizika, Praha, in Czech 2002.
Kosik-Bogacka D, Bukowska K, Kuźna-Grygiel W: Detection of Borrelia burgdorferi sensu lato in mosquitoes (Culicidae) in recreation areas of the city of Szczecin. Ann Agric Environ Med 2002, 9, 55-57.
Kosik-Bogacka D, Kuźna-Grygiel W, Bukowska K: The prevalence of spirochaete Borrelia burgdorferi sensu lato in ticks Ixodes ricinus and mosquitoes Aëdes spp. within a selected recreational area in the city of Szczecin. Ann Agric Environ Med 2004, 11, 105-108.
Kosik-Bogacka D, Kuźna-Grygiel W, Jamborowska M: Ticks and mosquitoes as a vectors of Borrelia burgdorferi s.l. in the forested areas of Szczecin. Folia Biol 2007, 55(3-4), 143-146.
Magnarelli LA, Freier JE, Anderson JF: Experimental infections of mosquitoes with Borrelia burgdorferi, the etiologic agent of Lyme disease. J Infect Dis 1987, 156, 694-695.
Picken MM, Picken RN, Han D, Cheng Y, Strle F: Single-tube nested polymerase chain reaction assay based on Flagellin gene sequences for detection of Borrelia burgdorferi sensu lato. Eur J Clin Microbiol Infect Dis 1996, 15, 489-498.
Žákovská A, Čapková L, Šerý O, Halouzka J, Dendis M: Isolation of Borrelia afzelii from overwintering Culex pipiens biotype molestus mosquitoes. Ann Agric Environ Med 2006, 13, 345-348.
Žákovská A, Nejedlá P, Holíková A, Dendis M: Positive findings of Borrelia burgdorferi in Culex (Culex) pipiens pipiens larvae in the surrounding of Brno city determined by the PCR method. Ann Agric Environ Med 2002, 9, 257-259.
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.