RESEARCH PAPER
Seroprevalence of Antibodies against Borrelia burgdorferi s. l. and Leptospira interrogans s. l. in Cats in district of Brno and its environs, the Czech Republic
 
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1
Faculty of Science, Masaryk University, Brno, Czech Republic
2
University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
CORRESPONDING AUTHOR
Pavel Schánilec   

University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
 
KEYWORDS
TOPICS
ABSTRACT
Objectives:
The aim of this study is to evaluate the seroprevalence of antibodies of Borrelia burgdorferi sensu lato (Bbsl) and Leptospira interrogans sensu lato (Lisl) and their possible concurrence in domestic cats living in variable conditions in South Moravia in the district of Brno and its environs. Additional objectives were to discover possible differences in seroprevalence between groups of cats living in different living conditions, and to determine the spectrum of Leptospira serogroups in cats in the same places.

Material and methods:
A total of 360 blood sera from domestic cats of 3 different sets were collected during the period 2013–2015. All samples were examined using ELISA for the detection of IgM and IgG antibodies against Bbsl, and the microscopic agglutination test (MAT) for the detection of antibodies against 8 serogroups of Lisl.

Results:
The ELISA method determined 15.8%, 4.8% and 10.3% IgM anti-Borrelia antibodies in the patient group, shelter cats and street cats, respectively. IgG anti-Borrelia antibodies were found in 6.2%, 9.5%, 5.2%, respectively. Antibodies specific for 5 Leptospira serogroups were detected by the use of MAT in 8.8%, 9.5% and 10.3% of cats from the investigated groups. The total positivity of all examined cats for anti-Borrelia antibodies was 18.0% and for anti-Leptospira – 9.2%.

Conclusions:
Cats can be infected with both Bbsl and Lisl. The obtained results are exclusive to the city of Brno and its environs, and are comparable to the limited previous studies. There is a need for further studies of clinical signs of both infections and the possible transmission of Leptospira by ticks.

 
REFERENCES (36)
1.
Greene CE, Straubinger RK, Levy SA. Borreliosis. In: Greene CE. Infectious diseases of the dog and cat. 4rd ed. St. Louis: Elsevier Saunders; 2012. p. 447–465.
 
2.
Sykes JE. Lyme Borreliosis. In: Sykes JE. Canine and feline infectious diseases. St. Louis: Elsevier Saunders; 2014. p. 487–497.
 
3.
Aberer E. Lyme borreliosis-an update. J Dtsch Dermatol Ges. 2007; 5: 406–414.
 
4.
Hovius EK. Borreliosis. In: Shaw SE, Day MJ. Arthropod-borne infectious diseases of the dog and cat. London: Manson Publishing, 2005. p. 100–109.
 
5.
Netušil J, Žákovská A, Vostal K, Norek A, Stanko M. The occurrence of Borrelia burgdorferi sensu lato in certain ectoparasites (Mesostigmata, Siphonaptera) of Apodemus flavicollis and Myodes glareolus in chosen localities in the Czech Republic. Acta Parasitol. 2013; 58: 337–341.
 
6.
Hubálek Z, Halouzka J, Juřicová Z. Investigation of haematophagous arthropods for borreliae – summarized data 1988–1996. Folia Parasitol. 1998; 45: 67–72.
 
7.
Netušil J, Žákovská A, Vostal K, Norek A, Stanko M. The occurrence of Borrelia burgdorferi sensu lato in certain ectoparasites (Mesostigmata, Siphonaptera) of Apodemus flavicollis and Myodes glareolus in chosen localities in the Czech Republic. Acta Parasitol. 2013; 58(3): 337–41.
 
8.
Rodriguez J, Blais MC, Lapointe C, Arsenault J, Carioto L, Harel J. Serologic and Urinary PCR Survey of Leptospirosis in Healthy Cats and in Cats with Kidney Disease. J Vet Intern Med. 2014; 28: 284–293.
 
9.
Littman MP, Gerber B, Goldstein RE, Labato MA, Lappin MR, Moore GE. ACVIM consensus update on Lyme borreliosis in dogs and cats. J Vet Intern Med. 2018; 32: 887–903. https://doi.org/10.1111/jvim.1....
 
10.
Birchard SJ, Sherding RG. Saunders manual of small animal practise. 3rd ed. St. Louis, Missouri: Elsevier, 2006.
 
11.
Brown RR, Elston TH, Evans L, Glaser C, Gulledge ML, Lappin MR, Marcus LC. Feline zoonoses guidelines from the American Association of Feline Practitioners. J Feline Med Surg. 2005; 7: 243–274.
 
12.
Rabinowitz PM, Gordon Z, Odofin L. Pet-related infections. Am Fam Physician. 2007; 76: 1314–1322.
 
13.
Greene CE, Sykes JE, Moore GE, Goldstein RE, Schultz RD. Leptospirosis. In: Greene CE. Infectious diseases of the dog and cat. 4rd ed. St. Louis: Elsevier Saunders; 2012. p. 431–447.
 
14.
Sykes JE. Leptospirosis. In: Sykes JE. Canine and feline infectious diseases. St. Louis: Elsevier Saunders; 2014. p. 474–486.
 
15.
Sykes JE, Hartmann K, Lunn KF, Moore GE, Stoddard RA, Goldstein RE. 2010 ACVIM Small Animal Consensus Statement on Leptospirosis: Diagnoses, Epidemiology, Treatment, and Prevention. J Vet Intern Med. 2011; 25: 1–13.
 
16.
Schuller S, Francey T, Hartmann K, Hugonnard M, Kohn B, Nally JE, et al. European consensus statement on leptospirosis in dogs and cats. J Small Anim Pract. 2015; 56: 159–179.
 
17.
Žákovská A, Nejezchlebová H, Bartoňková N, Rašovská T, Kučerová H, Norek A, Ovesná P. Activity of the tick Ixodes ricinus monitored in a suburban park in Brno, Czech Republic, in association with evaluation of selected repellents. Journal of Vector Ecology 2013; 38(2): 295–300.
 
18.
Vostal K, Žákovská A. Two-year study of Examination of Blood from Wild-living Rodents for the Presence of Antiborrelian Antibodies. Ann Agric Environ Med. 2003; 10: 1–4.
 
19.
Shropshire SB, Veir JK, Morris AK, Lappin MR. Evaluation of Leptospira species microscopic agglutination test in experimentally vaccinated cats and Leptospira species seropositivity in aged azotemic client-owned cats. J Feline Med Surg. 2016; 18(10): 768–772.
 
20.
Terpstra WJ. Human leptospirosis: Guidance for diagnosis, surveillance and control. World Health Organisation, 2003.
 
21.
Žákovská A, Nejezchlebová H, Bartoňková N, Rašovská T, Kučerová H, Norek A, et al. Activity of the tick Ixodes ricinus monitored in a suburban park in Brno, Czech Republic, in association with evaluation of selected repellents. J Vector Ecol. 2013; 38: 1–6.
 
22.
Fox JG, Anderson LC, Loew FM, Quimby F. Laboratory animal medicine. 2nd ed. London: Elsevier; 2002.
 
23.
Wójcik-Fatla A, Zając V, Cisak E, Sroka J, Sawczyn A, Dutkiewicz J. Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland. Ann Agric Environ Med. 2012; 19(4): 656–659.
 
24.
Azócar-Aedo L, Smits HL, Monti G. Leptospirosis in dogs and cats: epidemiology, clinical disease, zoonotic implications and prevention. Arch Med Vet. 2014; 46: 337–348.
 
25.
Sessions J, Greene C. Canine leptospirosis: epidemiology, pathogenesis and diagnosis. Comp Cont Educ Pract Vet. 2004; 26: 606–618.
 
26.
Euzéby, JP. List of Bacterial Names with Standing in Nomenclature: a Folder Available on the Internet. Int J Syst Bacteriol. 1997; 47: 590–592. doi:10.1099/00207713-47-2-590.
 
27.
Lappin MR. Polysystemic Bacterial Diseases: Leptospirosis. In: Nelson RW, Couto CG, Small Animal Internal Medicine. 4rd ed. St. Louis, Missouri: Mosby; 2009. p. 1315–1317.
 
28.
Songer SG, Post KW. Veterinary microbiology: Bacterial and fungal agents of animal disease. St. Louis, Missouri: Saunders Elsevier; 2005.
 
29.
Magnarelli LA, Bushmich SL, Ijdo JW, Fikrig E. Seroprevalence of antibodies against Borrelia burgdorferi and Anaplasma phagocytophilum in cats. Am J Vet Res. 2005; 66: 1895–1899.
 
30.
Levy SA, O’Connor TP, Hanscom JL, et al. Evaluation of a canine C6 ELISA Lyme disease test for the determination of the infection status of cats naturally exposed to Borrelia burgdorferi. Vet Ther. 2003; 4: 172–177.
 
31.
Pantchev N, Vrhovec MG, Pluta S, Straubinger RK. Seropositivity of Borrelia burgdorferi in a cohort of symptomatic cats from Europe based on a C6-peptide assay with discussion of implications in disease aetiology. Berl Munch Tierarztl Wochenschr. 2016; 129(7–8): 333–339.
 
32.
Burgess EC. Experimentally induced infection of cats with Borrelia burgdorferi. Am J Vet Res. 1992; 53: 1507–1511.
 
33.
Colville JL, Berryhill DL. Handbook of zoonoses: identification and prevention. St. Louis, Missouri: Mosby; 2007.
 
34.
Magnarelli LA, Anderson JF, Levine HR, et al. Tick parasitism and antibodies to Borrelia burgdorferi in cats. J Am Vet Med Assoc. 1990; 197: 63–66.
 
35.
Larsson CE, Santa Rosa CA, Hagiwara MK, Paim GV, Guerra JL. Prevalence of feline leptospirosis: serologic survey and attempts of isolation and demonstration of the agent. Int J Zoonoses. 1984; 11: 161–169.
 
36.
Agunloye CA, Nash AS. Investigation of possible leptospiral infection in cats in Scotland. J Small Anim Pract. 1996; 37: 126–129.
 
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