Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland
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Department of Zoonoses, Institute of Rural Health, Lublin, Poland
Department of Parasitology, National Veterinary Research Institute, Puławy, Poland
Angelina Wójcik-Fatla   

Department of Zoonoses, Institute of Rural Health, Lublin, Poland
Ann Agric Environ Med. 2012;19(4):656–659
A total of 836 unfed Ixodes ricinus ticks were collected from 2 forested areas of the Lublin region in eastern Poland. Of these, 540 ticks were collected in area ‘A’, exposed to flooding from the Vistula river, while the remaining 296 ticks were collected in suburban area ‘B’, not exposed to flooding. Ticks were examined by nested-PCR for the presence of DNA of Leptospira spp. and of Borrelia burgdorferi sensu lato, including its genospecies. The presence of the Leptospira spp. DNA was found in the examined specimens of Ixodes ricinus. The infection rate was much greater in area ‘A’ exposed to flooding, compared to unexposed area ‘B’ (15.6% vs. 1.4%, p<0.0001). A significant difference was noted in the case of all developmental stages. For the total results, the prevalence of Leptospira spp. in nymphs (16.9%) was two-fold greater (p<0.01) than in females and males (7.9% and 7.1%, respectively). The total prevalence of B. burgdorferi sensu lato in examined ticks amounted to 24.3%. Altogether, the genospecies Borrelia burgdorferi sensu stricto was detected most often. No correlation was found to exist between the presence of Leptospira spp. and B. burgdorferi sensu lato in the examined ticks, which indicates that the detection of Leptospira in ticks was not due to a false-positive cross-reaction with DNA of B. burgdorferi. In conclusion, this study shows for the first time the presence of Leptospira spp. in Ixodes ticks and marked frequency of the occurrence of these bacteria in ticks. This finding has significant epidemiological implications by indicating the possibility of the transmission of leptospirosis by Ixodes ricinus, the commonest tick species in Europe and most important vector of numerous pathogens.
Evangelista KV, Coburn J. Leptospira as an emerging pathogen: a review of its biology, pathogenesis and host immune responses. Future Microbiol. 2010; 5:1413-1425.
Adler B, de la Peña Moctezuma A. Leptospira and leptospirosis. Vet Microbiol. 2010; 140: 287-296.
Levett PN. Leptospirosis. Clin Microbiol Rev. 2001; 14: 296-325.
Vijayachari P, Sugunan AP, Shriram AN. Leptospirosis: an emerging global public health problem. J Biosci. 2008; 33: 557-569.
Cruz LS, Vargas R, Lopes AA. Leptospirosis: a worldwide resurgent zoonosis and important cause of acute renal failure and death in developing nations. Ethn Dis. 2009; 19(Suppl 1): S1-37-41.
Pappas G, Papadimitriou P, Siozopoulou V, Christou L, Akritidis N. The globalization of leptospirosis: worldwide incidence trends. Int J Infect Dis. 2008; 12: 351-357.
Hartskeerl RA, Collares-Pereira M, Ellis WA. Emergence, control and re-emerging leptospirosis: dynamics of infection in the changing world. Clin Microbiol Infect. 2011; 17: 494-501.
Dutkiewicz J, Cisak E, Sroka J, Wójcik-Fatla A, Zając V. Biological agents as occupational hazards – selected issues. Ann Agric Environ Med. 2011; 18: 286-293.
Wasiński B, Sroka J, Wójcik-Fatla A, Zając V, Cisak E, Knap JP, Sawczyn A, Dutkiewicz J. Seroprevalence of leptospirosis in rural populations inhabiting areas exposed and not exposed to floods in eastern Poland. Ann Agric Environ Med. 2012; 19: 285-288.
Lau CL, Smythe LD, Craig SB, Weinstein P. Climate change, flooding, urbanisation and leptospirosis: fuelling the fire? Trans R Soc Trop Med Hyg. 2010; 104: 631-638.
Burgdorfer W. The possible role of ticks as vectors of leptospirae. I. Transmission of Leptospira pomona by the argasid tick, Ornithodoros turicata, and the persistence of this organism in its tissues. Exp Parasitol. 1956; 5: 571-579.
Burgdorfer W. The possible role of ticks as vectors of Leptospirae. II. Infection of the ixodid ticks, Dermacentor andersoni and Amblyomma maculatum, with Leptospira pomona. Exp Parasitol. 1959; 8: 502-508.
Krepkogorskaya TA, Rementsova MM. Isolation of Leptospira strains from the ticks Dermacentor marginatus S. collected from cattle. Zh Mikrobiol Epidemiol Immunobiol. 1957; 28(2):93-94 (in Russian).
Gern L. The biology of the Ixodes ricinus tick. Ther Umsch. 2005; 62: 707-712 (in German).
Stanek G. Pandora’s Box: pathogens in Ixodes ricinus ticks in Central Europe. Wien Klin Wochenschr. 2009; 121: 673-683 (in German).
Bartosik K, Lachowska-Kotowska P, Szymańska J, Wójcik-Fatla A, Pabis A, Buczek A. Environmental conditioning of incidence of tick-borne encephalitis in the south-eastern Poland in 1996-2006. Ann Agric Environ Med. 2011; 18: 119-126.
Bartosik K, Lachowska-Kotowska P, Szymańska J, Pabis A, Buczek A. Lyme borreliosis in south-eastern Poland: relationships with environmental factors and medical attention standards. Ann Agric Environ Med. 2011; 18: 131-137.
Bartosik K, Sitarz M, Szymańska J, Buczek A. Tick bites on humans in the agricultural and recreational areas in south-eastern Poland. Ann Agric Environ Med. 2011; 18: 151-157.
Rijpkema S, Golubic D, Moelkenboer M, Verbeek-De Kruif N, Schellekens J. Identification of four genomic groups of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in a Lyme borreliosis endemic region of northern Croatia. Exp Appl Acarol. 1996; 20: 23-30.
Amutha R, Chaudhury P, Garg AP, Vasan P, Cheema PS, Srivastava SK. Cloning and sequence analysis of the gene encoding LipL32 of Leptospira interrogans serovar Sejroe. Vet Res Commun. 2007; 31: 513-519.
Wójcik-Fatla A, Szymańska J, Wdowiak L, Buczek A, Dutkiewicz J. Coincidence of three pathogens (Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti) in Ixodes ricinus ticks in the Lublin macroregion. Ann Agric Environ Med 2009; 16: 151-158.
Cisak E, Wójcik-Fatla A, Zając V, Sroka J, Dutkiewicz J. Risk of Lyme disease at various sites and workplaces of forestry workers in eastern Poland. Ann Agric Environ Med 2012; 19: 465-468.
Cisak E, Wójcik-Fatla A, Stojek NM, Chmielewska-Badora J, Zwoliński J, Buczek A, Dutkiewicz J. Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin region (Eastern Poland). Ann Agric Environ Med. 2006; 12: 301-306.
Cisak E, Chmielewska-Badora J, Zwoliński J, Wójcik-Fatla A, Zając V, Skórska C, Dutkiewicz J. Study on Lyme borreliosis focus in the Lublin region (Eastern Poland). Ann Agric Environ Med. 2008; 15: 327-332.