RESEARCH PAPER
Occurrence of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in Ixodes ricinus ticks collected from selected areas of Opolskie Province in south-west Poland
 
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Department of Parasitology, Medical University of Silesia, Katowice, Poland
 
 
Corresponding author
Marek Asman   

Medical University of Silesia in Katowice, Department of Parasitology, Jedności 8, 41-200, Sosnowiec, Poland
 
 
Ann Agric Environ Med. 2019;26(4):544-547
 
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Ticks (Acari: Ixodida) are vectors and/or reservoirs of many pathogens, i.e. Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti. These pathogens are ethiological agents of such diseases as Lyme borreliosis, human granulocytic anaplasmosis and human babesiosis.

Objective:
The aim of the study was to evaluate the role of the Ixodes ricinus in the transmission of Borrelia burgdorferi sensu lato, Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto, Anaplasma phagocytophilum and Babesia microti in Opolskie Province in Poland.

Material and methods:
DNA from 222 ticks was isolated by the ammonia method. The pair of primers specific to the flagelline gene was used to detect of B. burgdorferi s. l. To detect of genospecies of this spirochete, three pairs of internal primers were used. In turn, two pairs of primers specific to the 16S rDNA gene and the 18S rRNA were used, respectively, for the detection of A. phagocytophilum and B. microti. Borrelia burgdorferi s. l., A. phagocytophilum, and B. microti were detected in 4.5%, 2.7% and 5.4% of examined ticks, respectively.

Results and conclusions:
Of the ten ticks infected with B. burgdorferi s. l., B. afzelii was found in seven, undefinied genospecies in two, and mixed infection with B. afzelii and B. burgdorferi s. s. in one. The study demonstrated the potential risk of exposure of humans and animals to infections of B. burgdorferi s. l., A. phagocytophilum and B. microti in the examined area of Poland.

 
REFERENCES (34)
1.
Nowak-Chmura M. Fauna of ticks (Ixodida) of Central Europe. Wydawnictwo Naukowe Uniwersytetu Pedagogicznego Kraków, 2013.
 
2.
Burgdorfer W, Barbour AG, Hayes SF, Benach JL, Grunwaldt E, Davis JP. Lyme disease-a-tick-borne spirochetosis? Science. 1982; 216: 1317–1319.
 
3.
Petrovec M, Lotric-Furlan S, Zupanc TA, Strle F, Brouqui Ph, Roux V, Dumler JS. Human disease in Europe caused by a granulocytic Ehrlichia species. J Clin Microbiol. 1997; 35: 1556–1559.
 
4.
Boustani MR, Gelfand JA. Babesiosis. Clin Infect Dis. 1995; 22: 611–615.
 
5.
Kiewra D. The evaluation of the vector role of Ixodes ricinus L. 1758 ticks (Acari: Ixodidae) in the transmission of Borrelia burgdorferi s. l. spirochetes in Poland with particular emphasis on the Lower Silesia. I-Bis Wrocław, 2014.
 
6.
Asman M, Gąsior T, Pająk C, Cuber P, Szilman P, Szilman E, Solarz K. Occupational risk of infections with Borrelia burgdorferi sensu lato, B. burgdorferi sensu stricto, B. garinii and B. afzelii in agricultural workers on the territory of Beskid Żywiecki. In: Buczek A, Błaszak Cz, editors. Arthropods: Medical and economical significance. Akapit, Lublin, 2012. p. 163–170.
 
7.
Januszkiewicz J, Kieda A. Cases of borreliosis (Lyme disease) in Western Pomerania. Przeg Epidemiol. 1987; 41: 324–329.
 
8.
Kiewra D, Dobacki W, Lonc E, Dobracka B. Zagrożenie boreliozą z Lyme na Dolnym Śląsku. In: Buczek A, Błaszak Cz, editors. Arthropods: Parasite – host relationships. Liber, Lublin, 2004. p. 202–207.
 
9.
Bartosik K, Lachowska-Kotowska P, Szymańska J, Pabis A, Buczek A. Lyme borreliosis in south-eastern Poland: relationships with environment al factors and medical attention standards. Ann Agric Environ Med. 2011; 18: 131–137.
 
10.
Mannelli A, Bertolotti L, Gern L, Gray J. Ecology of Borrelia burgdorferi sensu lato in Europe: transmission dynamics in multi-host system, influence of molecular processes and effects of climate change. Microbiol Rev. 2012; 36: 837–861.
 
11.
Balmelli T, Piffaretti JC. Association between different clinical manifestations of Lyme disease and different species of Borrelia burgdorferi sensu lato. Res Microbiol. 1995; 146: 329–340.
 
12.
Stańczak J, Kubica – Biernat B, Racewicz M, Kruminis-Łozowska W, Kur J. Detection of three genospecies of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in different regions of Poland. Int J Med Microbiol. 2000; 290: 559–566.
 
13.
Asman M, Nowak M, Cuber P, Strzelczyk J, Szilman E, Szilman P, Trapp G, Siuda K, Solarz K, Wiczkowski A. The risk of exposure to Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, Babesia sp. and co-infections in Ixodes ricinus ticks on the territory of Niepołomice Forest (southern Poland). Ann Parasitol. 2013; 59(1): 13–19.
 
14.
Tylewska-Wierzbanowska S, Chmielewski T, Kondrusik M, Hermanowska-Szpakowicz T, Sawicki W, Sułek K. First cases of acute human granulocytic ehrlichiosis in Poland. Eur J Clin Microbiol Infect Dis. 2001; 20: 196–198.
 
15.
Tomasiewicz K, Modrzewska R, Buczek A, Stańczak J, Maciukajć J. The risk of exposure to Anaplasma phagocytophilum infection in mid-eastern Poland. Ann Agric Environ Med. 2004; 11: 261–264.
 
16.
Asman M, Solarz K, Szilman E, Szilman P, Sikora B, Jakubas-Zawalska J. The occurrence of three tick-borne pathogens in Ixodes ricinus ticks collected from the area of the Kraków-Częstochowa Upland (Southern Poland). Acarologia 2018; 58(4): 967–975.
 
17.
Humiczewska M, Kuźna-Grygiel W. The case of imported human babesiosis in Poland. Wiad Parazytol. 1997; 43: 227–229.
 
18.
Skotarczak B. Babeszjoza człowieka i psa domowego; etiologia, chorobotwórczość, diagnostyka. Wiad Parazytol. 2007; 53: 271–280.
 
19.
Rijpkema S, Golubi D, Molkenboer M, Verbreek-de Kruif N, Schellekens J. Identification of four genomic groups of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks in a Lyme borreliosis endemic region of northern Croatia. Exp Appl Acarol. 1996; 20: 23–30.
 
20.
Wodecka B, Skotarczak B. Genetic diversity of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected in north-west Poland. Wiad Parazytol. 2000; 46: 475–485.
 
21.
Massung RF, Slater K, Owens JH, Nicholson WL, Mather TN, Solberg VB and Olson JG. Nested PCR Assay for detection of granulocytic ehrlichiae. J Clin Microbiol. 1998; 36(4): 1090–1095.
 
22.
Persing DH, Mathiesen D, Marshall WF, Telford SR, Spielman A, Thomford JW, Conrad PA. Detection of Babesia microti by polymerase chain reaction. J Clin Microbiol. 1992; 30: 2097–2103.
 
23.
Strzelczyk JK, Gaździcka J, Cuber P, Asman M, Trapp G, Gołabek K, Zalewska Ziob M, Nowak Chmura M, Siuda K, Wiczkowski A, Solarz K. Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from southern Poland. Acta Parasitol. 2015; 60(4): 666–674.
 
24.
Bartosik K, Lachowska-Kotowska P, Szymańska J, Pabis A, Buczek A. Lyme borreliosis in south-eastern Poland: Relationships with environmental factors and medical attention standards. Ann Agric Environ Med. 2011; 18:131–137.
 
25.
Hubalek Z, Halouzka J, Juricova Z, Sikutova S, Rudolf I. Effect of forest cleaning on the abundance of Ixodes ricinus ticks and the prevalence Borrelia burgdorferi s.l. Med Vet Entomol. 2006; 20: 166–172.
 
26.
Wodecka B. Fla B gene as a molecular marker for distinct identification of Borrelia species in environmental samples by the PCR-Restriction Fragment Length Polymorphism. Appl Environ Microb. 2011; 77(19): 7088–7092.
 
27.
Cisak E, Wójcik-Fatla A, Stojek NM, Chmielewska-Badora J, Zwoliński J, Buczek A, Dutkiewicz J. Prevalence of Borrelia burgdorferi genospecies in Ixodes ricinus ticks from Lublin region (Eastern Poland). Ann Agric Environ Med. 2006; 13: 301–306.
 
28.
Blanco JR, Oteo JA. Human granulocytic ehrlichiosis in Europe. Clin Microbiol Infect. 2002; 8: 763–772.
 
29.
Kiewra D, Zaleśny G, Czułowska A. The risk of infection with Anaplasma phagocytophilum and Babesia microti in Lower Silesia, SW Poland. In: Buczek A, Błaszak Cz, editors. Arthropods: Threat to human and animals health. Koliber, Lublin, 2014. p. 103–110.
 
30.
Stańczak J, Mohammed Gabre R, Kruminis-Łozowska W, Racewicz M, Kubica-Biernat B. Ixodes riciuns as a vector of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Babesia microti in urban and suburban forests. Ann Agric Environ Med. 2004; 11: 109–114.
 
31.
Cisak E, Chmielewska-Badora J, Zwoliński J, Wójcik-Fatla A, Polak J, Dutkiewicz J. Risk of tick-borne bacterial diseases workers of Roztocze National Park (south-eastern Poland). Ann Agric Environ Med. 2005; 12: 127–132.
 
32.
Chmielewska-Badora J, Zwoliński J, Cisak E, Wójcik-Fatla E, Buczek A, Dutkiewicz J. Prevalence of Anaplasma phagocytophilum in Ixodes ricinus ticks determined by polymerase chain reaction with two pair of primers detecting 16S rRNA and ankA genes. Ann Agric Environ Med. 2007; 14: 281–285.
 
33.
Asman M, Solarz K, Cuber P, Gąsior T, Szilman P, Szilman E, Tondaś E, Matzullok A, Kusion N, Florek K. Detection of protozoans Babesia microti and Toxoplasma gondii and their co-existence in ticks (Acari: Ixodida) collected in Tarnogórski district (Upper Silesia, Poland). Ann Agric Environ Med. 2015; 22(1): 80–83.
 
34.
Wójcik-Fatla A, Cisak E, Chmielewska-Badora J, Zwoliński J, Buczek A, Dutkiewicz J. Prevalence of Babesia microti in Ixodes ricinus ticks from Lublin region (Eastern Poland). Ann gric Environ Med. 2006; 13: 319–322.
 
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