Blastocystis spp. has been reported in wildlife, domestic animals and animals housed in ZOO. To-date, 17 genetically diverse lines have been reported in mammals and birds (designated ST) based on differences in the SSU rRNA. In this study, faeces samples were collected from 24 ZOO animals with clinical signs suggestive of gastrointestinal disease in Košice ZOO, Slovakia. After DNA isolation, PCR was conducted to amplify the SSU region of DNA of Blastocystis species. Forward primer- Blast F and reverse primer- Blast R were used in the reaction. From 25 faeces samples, Blastocystis spp. was detected in 5 animals (3 mammals, 2 birds), with a prevalence of 20%. Subsequent molecular analyses identified the ST 5 (n = 3), ST 7 (n = 1), and ST 12 (n = 1) subtypes, where the ST 5 subtype was identified in the mammalian group and birds, and the ST 7 and ST 12 subtypes were identified only in mammals. Based on these findings, focusing on ZOO animals as a potential source of infection for humans is highly recommended.
This project was created with the support of the grant project of the Ministry of Education, Science, Research and Sport of the Slovak Republic VEGA no. 1/0113/20.
Abe N. Molecular and phylogenetic analysis of Blastocystis isolates from various hosts. Vet Parasitol. 2004; 120(3): 235–242.
Stensvold CR, et al. Subtype distribution of Blastocystis isolates from synanthropic and ZOO animals and identification of a new subtype. Int J Parasitol. 2009; 39(4): 473–9.
Parkar U, et al. Molecular characterization of Blastocystis isolates from ZOO animals and their animal-keepers. Vet Parasitol. 2010; 169(1–2): 817.
Roberts T, et al. Subtype distribution of Blastocystis isolates from a variety of animals from New South Wales, Australia. Vet Parasitol. 2013; 196(1–2): 85–9.
Yoshikawa H, Iwamasa A. Human Blastocystis subtyping with subtypespecific primers developed from unique sequences of the SSU rRNA gene. Parasitol Int. 2016; 65(6 Pt B): 785–791.
Noel C, et al. Molecular phylogenies of Blastocystis isolates from different hosts: implications for genetic diversity, identification of species, and zoonosis. J Clin Microbiol. 2005; 43(1): 348–55.
Betts EL, Gentekaki E, Tsaousis AD. Exploring Micro-Eukaryotic Diversity in the Gut: Co-occurrence of Blastocystis Subtypes and Other Protists in ZOO Animals. Front Microbiol. 2020;11:288. Published 2020 Feb 25. doi:10.3389/fmicb.2020.00288.
Ramirez JD, et al. Geographic distribution of human Blastocystis subtypes in South America. Infect Genet Evol. 2016; 41: 32–35.
Ruaux CG, Stang BV. Prevalence of blastocystis in shelter-resident and client-owned companion animals in the US Pacific Northwest. PLoS One. 2014; 9(9): e107496.
Schar F, et al. The prevalence and diversity of intestinal parasitic infections in humans and domestic animals in a rural Cambodian village. Parasitol Int. 2014; 63(4): 597–603.
Figueroa J. New records of parasites in free-ranging Andean bears from Peru. Ursus. 2015; 26(1): 21–27.
Puebla LEJ, et al. Prevalence of intestinal parasites and molecular characterization of Giardia duodenalis from dogs in La Habana, Cuba. Vet Parasitol Reg Stud Reports. 2017; 8: 107–112.
Betts EL, et al. Genetic diversity of Blastocystis in non-primate animals. Parasitol. 2018; 145(9): 1228–1234.
Alfellani MA, et al. Genetic diversity of blastocystis in livestock and ZOO animals. Protist. 2013; 164(4): 497–509.
Danisova O, Valencakova A. First detection of Blastocystis sp. in pigs in Slovakia and in Europe. Parasitol Int. 2021; 81: 102235.
Zanzani SA, et al. Study of the gastrointestinal parasitic fauna of captive nonhuman primates (Macaca fascicularis). Parasitol Res. 2016; 115(1): 307–12.
Helenbrook WD, Shields WM, Whipps CM. Characterization of Blastocystis species infection in humans and mantled howler monkeys, Alouatta palliata aequatorialis, living in close proximity to one another. Parasitol Res. 2015; 114(7): 2517–2525.
Kalousova B, et al. Gastrointestinal Parasites of Savanna Chimpanzees (Pan troglodytes schweinfurthii) in Ugalla, Tanzania. Int J Primatol. 2014; 35(2): 463–475.
Petrasova J, et al. Diversity and host specificity of Blastocystis in syntopic primates on Rubondo Island, Tanzania. Int J Parasitol. 2011; 41(11): 1113–20.
Abe N, et al. A survey of Blastocystis sp. in livestock, pets, and ZOO animals in Japan. Vet Parasitol. 2002; 106(3): 203–12.
Alfellani MA, et al. Diversity and distribution of Blastocystis sp. subtypes in nonhuman primates. Parasitol. 2013; 140(8): 966–71.
Cian A, et al. Molecular Epidemiology of Blastocystis sp. in Various Animal Groups from Two French Zoos and Evaluation of Potential Zoonotic Risk. PLoS One. 2017; 12(1): e0169659.
Li XD, et al. Prevalence and subtypes of Blastocystis sp. infection in ZOO animals in three cities in China. Parasitol Res. 2020; 119(2): 465–471.
Alfellani MA, et al. Variable geographic distribution of Blastocystis subtypes and its potential implications. Acta Trop. 2013; 126(1): 11–8.
Parkar U, et al. Direct characterization of Blastocystis from faeces by PCR and evidence of zoonotic potential. Parasitol. 2007; 134(Pt 3): 359–67.