Introduction and objective:
Anaplasma bacteria are intracellular, gram-negative microorganisms transmitted by ticks that can pose a threat to the health of both animals and humans. In our geographical conditions, the transmission of Anaplasmas occurs mainly through ticks of the species Ixodes ricinus, which represent the most abundant species in Slovakia. The aim of the study is to investigate the seroprevalence of Anaplasma spp. antibodies in cattle, sheep and goats across Slovakia.

Material and methods:
The study involved serological testing of 156 cattle, 124 sheep and 104 goats from various regions of Slovakia. A total of 384 serum samples were analysed through the use of the competitive ELISA method.

Results:
The seropositivity was 10.90% in cattle, 70.16% in sheep and 43.27% in goats.

Conclusions:
The study additionally identified regional variations, indicating that environmental conditions, vector ecology, and animal management practices, play a role in the transmission of Anaplasma spp. These findings emphasise the need for targeted strategies for the prevention and control of anaplasmosis, tailored to specific species and regions, to reduce its economic and health impacts on livestock. The results contribute to the growing body of knowledge on Anaplasma epidemiology, highlighting the importance of ongoing surveillance to manage its effects. Given the zoonotic potential of anaplasmosis, it is important to analyse the results obtained also from the One Health perspective.