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RESEARCH PAPER
The oral microbiota change in oral cancer – a preliminary study
1
Clinic of Maxillofacial Surgery, Medical University, Lublin, Poland
2
Department of Pharmaceutical Microbiology, Medical University, Lublin, Poland
3
Department of Oral Surgery, Medical University, Lublin, Poland
4
Department of Experimental Immunology, Medical University, Lublin, Poland
5
Paediatric Otolaryngology Clinic, SZPZOZ im. Dzieci Warszawy w Dziekanów Leśny, Poland
Corresponding author
KEYWORDS
TOPICS
ABSTRACT
Introduction and objective:
The relationship between oral bacteria and oral squamous cell carcinoma (OSCC) is not yet fully understood. This study aimed to profile the microbiota composition at tumour sites versus adjacent normal tissues in the oral mucosa of 10 OSCC patients using a qPCR Array.
Material and methods:
We recruited 10 OSCC patients (8 men and 2 women, aged 40 to 89 years) from the Department of Oral Surgery at the Medical University of Lublin. The diagnosis of OSCC was confirmed through clinical presentation and histopathology. Bilateral swabs were taken for further examination. The study identified 93 microorganisms using the Oral Disease Microbial DNA qPCR Array.
Results:
From the 20 samples analyzed, we retrieved 868 species/genes across 37 genera, representing 90 different microbial species. The relative abundance of Fusobacterium nucleatum was significantly higher in OSCC samples (P < 0.05). Receiver operating characteristic (ROC) curve analysis identified F. nucleatum as a potential OSCC biomarker (p = 0.007). Correlation analysis revealed distinct ecological relationships within the bacterial communities of OSCC samples, consistent with the observed bacterial diversity.
Conclusions:
Notably, associations centred around Fusobacterium spp. were prominent in cancer samples, further suggesting a potential role for this genus in OSCC development. The differences in oral bacterial profiles between tumour and non-tumour tissues may serve as diagnostic markers.
The relationship between oral bacteria and oral squamous cell carcinoma (OSCC) is not yet fully understood. This study aimed to profile the microbiota composition at tumour sites versus adjacent normal tissues in the oral mucosa of 10 OSCC patients using a qPCR Array.
Material and methods:
We recruited 10 OSCC patients (8 men and 2 women, aged 40 to 89 years) from the Department of Oral Surgery at the Medical University of Lublin. The diagnosis of OSCC was confirmed through clinical presentation and histopathology. Bilateral swabs were taken for further examination. The study identified 93 microorganisms using the Oral Disease Microbial DNA qPCR Array.
Results:
From the 20 samples analyzed, we retrieved 868 species/genes across 37 genera, representing 90 different microbial species. The relative abundance of Fusobacterium nucleatum was significantly higher in OSCC samples (P < 0.05). Receiver operating characteristic (ROC) curve analysis identified F. nucleatum as a potential OSCC biomarker (p = 0.007). Correlation analysis revealed distinct ecological relationships within the bacterial communities of OSCC samples, consistent with the observed bacterial diversity.
Conclusions:
Notably, associations centred around Fusobacterium spp. were prominent in cancer samples, further suggesting a potential role for this genus in OSCC development. The differences in oral bacterial profiles between tumour and non-tumour tissues may serve as diagnostic markers.
REFERENCES (35)
1.
Kawakita D, Oze I, Iwasaki S, et al. Trends in the incidence of head and neck cancer by subsite between 1993 and 2015 in Japan. Cancer Med. 2022;11:1553–60. https://doi.org/10.1002/cam4.4....
2.
Global Cancer Observatory (2022). Oral cancer fact sheet.** Retrieved from [GCO](https://gco.iarc.fr/today/data...).
3.
Ferlay J, Colombet M, Soerjomataram I, et al. Cancer statistics for the year 2020: An overview. Int J Cancer. 2021;149:778–89. https://doi.org/10.1002/ijc.33....
4.
Warnakulasuriya S. Global epidemiology of oral and oropharyngeal cancer. Oral Oncol. 2009;45:309–16. https://doi.org/10.1016/j.oral....
5.
Sarode GS, Sarode SC, Maniyar N, et al. Oral cancer databases: A comprehensive review. J Oral Pathol Med. 2018;47:547–556. https://doi.org/10.1111/jop.12....
6.
Wołącewicz M, Becht R, Grywalska E, et al. Herpesviruses in Head and Neck Cancers. Viruses. 2020;12(2):172. doi: 10.3390/v12020172.
7.
Sedghi L, DiMassa V, Harrington A, et al. The oral microbiome: Role of key organisms and complex networks in oral health and disease. Periodontol 2000. 2021;87:107–131. https://doi.org/10.1111/prd.12....
8.
Tang JA, Amadio G, Ridge JA. Clinical and Histologic Features Associated With Malignant Transformation of Oral Cavity Dysplasia Managed at a Single North American Institution. JAMA Otolaryngol Head Neck Surg. 2022;148:28–34. doi:10.1001/jamaoto.2021.2755.
9.
Bakhti SZ, Latifi-Navid S. Oral microbiota and Helicobacter pylori in gastric carcinogenesis: what do we know and where next? BMC Microbiol. 2021;21:1–15. https://doi.org/10.1186/s12866....
10.
Vyhnalova T, Danek Z, Gachova D, et al. The role of the oral microbiota in the etiopathogenesis of oral squamous cell carcinoma. Microorganisms 2021;9:1549. https://doi.org/10.3390/microo....
11.
Sun J, Tang Q, Yu S, et al. Role of the oral microbiota in cancer evolution and progression. Cancer Med. 2020;9:6306–21. https://doi.org/10.1002/cam4.3....
12.
Perera M, Al-Hebshi NN, Speicher DJ, et al. Emerging role of bacteria in oral carcinogenesis: a review with special reference to perio-pathogenic bacteria. J Oral Microbiol. 2016;8:32762. https://doi.org/10.3402/jom.v8....
13.
Brierly G, Celentano A, Breik O, et al. Tumour Necrosis Factor Alpha (TNF-α) and Oral Squamous Cell Carcinoma. Cancers (Basel). 2023;15:1841. https://doi.org/10.3390/cancer....
14.
Yang K, Wang Y, Zhang S, et al. Oral Microbiota Analysis of Tissue Pairs and Saliva Samples From Patients With Oral Squamous Cell Carcinoma – A Pilot Study. Front Microbiol. 2021;12:719601. https://doi.org/10.3389/fmicb.....
15.
Zhao H, Chu M, Huang Z, et al. Variations in oral microbiota associated with oral cancer. Sci Rep. 2017;7:11773. https://doi.org/10.1038/s41598....
16.
Pratap Singh R, Kumari N, Gupta S, et al. Intratumoural Microbiota Changes with Tumour Stage and Influences the Immune Signature of Oral Squamous Cell Carcinoma. Microbiol Spectr. 2023;11:04596–22. https://doi.org/10.1128/spectr....
17.
Sarkar P, Malik S, Laha S, et al. Dysbiosis of Oral Microbiota During Oral Squamous Cell Carcinoma Development. Front Oncol. 2021;11:614448. https://doi.org/10.3389/fonc.2....
18.
Zhang L, Liu Y, Zheng HJ, et al. The Oral Microbiota May Have Influence on Oral Cancer. Front Cell Infect Microbiol. 2020;9:476. https://doi.org/10.3389/fcimb.....
19.
Pushalkar S, Mane SP, Ji X, et al. Microbial diversity in saliva of oral squamous cell carcinoma. FEMS Immunol Med Microbiol. 2011;61:269–77. DOI: 10.1111/j.1574–695X.2010.00773.x.
20.
Schmidt BL, Kuczynski J, Bhattacharya A, et al. Changes in Abundance of Oral Microbiota Associated with Oral Cancer. PLoS ONE 2014; 9: e98741. https://doi.org/10.1371/journa....
21.
Al-Hebshi NN, Nasher AT, Maryoud MY, et al. Inflammatory bacteriome featuring Fusobacterium nucleatum and Pseudomonas aeruginosa identified in association with oral squamous cell carcinoma. Sci Rep. 2017;7:1834. https://doi.org/10.1038/s41598....
22.
Pushalkar S, Ji X, Li Y, et al. Comparison of oral microbiota in tumour and non-tumour tissues of patients with oral squamous cell carcinoma. BMC Microbiol. 2012;12:144. https://doi.org/10.1186/1471–2....
23.
Binder Gallimidi A, Fischman S, Revach B, et al. Periodontal pathogens Porphyromonas gingivalis and Fusobacterium nucleatum promote tumour progression in an oral-specific chemical carcinogenesis model. Oncotarget. 2015;6:22613–23. https://doi.org/10.18632/oncot....
24.
Chang C, Geng F, Shi X, et al. The prevalence rate of periodontal pathogens and its association with oral squamous cell carcinoma. Appl Microbiol Biotechnol. 2019;103:1393–404. doi: 10.1007/s00253–018–9475–6.
25.
Mandal DP, Mohanty N, Behera PK, et al. A plausible proposition of CCL20-related mechanism in fusobacterium nucleatum-associated oral carcinogenesis. Life 2021;11:1218. https://doi.org/10.3390/life11....
26.
Nobbs AH, Jenkinson HF, Jakubovics NS. Stick to your gums: mechanisms of oral microbial adherence. J Dent Res. 2011;90:1271–8. https://doi.org/10.1177/002203....
27.
Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL Jr. Microbial complexes in subgingival plaque. J Clin Periodontol. 1998;25:134–44. https://doi.org/10.1111/j.1600....
28.
Buduneli, N. Environmental Factors and Periodontal Microbiome. Periodontol. 2000; 2021;85:112–125. https://doi.org/10.1111/prd.12....
29.
McIlvanna E, Linden GJ, Craig SG, et al. Fusobacterium nucleatum and oral cancer: a critical review. BMC Cancer 2021;21:1212. https://doi.org/10.1186/s12885....
30.
Gopinath D, Wie CC, Banerjee M, et al. Compositional profile of mucosal bacteriome of smokers and smokeless tobacco users. Clin Oral Investig. 2022;26:1647–1656. https://doi.org/10.1007/s00784....
31.
Fulbright LE, Ellermann M, Arthur JC. The microbiome and the hallmarks of cancer. PLoS Pathog. 2017;13:e1006480. https://doi.org/10.1371/journa....
32.
Shang YF, Shen YY, Zhang MC, et al. Progress in salivary glands: Endocrine glands with immune functions. Front Endocrinol (Lausanne). 2023;14:1061235. https://doi.org/10.3389/fendo.....
33.
Widyarman AS, Theodorea CF, Udawatte NS, et al. Diversity of Oral Microbiome of Women From Urban and Rural Areas of Indonesia: A Pilot Study. Front Oral Health. 2021;29(2):738306. https://doi.org/10.3389/froh.2....
34.
Ma G, Qiao Y, Shi H, et al. Comparison of the Oral Microbiota Structure among People from the Same Ethnic Group Living in Different Environments. Biomed Res Int. 2022;2022:6544497. https://doi.org/10.1155/2022/6....
35.
Ramamurthy P, Sharma D, Clough A, et al. Influence of Rurality on Oral Cancer Trends among Organisation for Economic Co-Operation and Development (OECD) Member Countries-A Scoping Review. Cancers (Basel). 2024;16(17):2957. https://doi.org/10.3390/cancer....
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| eISSN: | 1898-2263 |
| ISSN: | 1232-1966 |
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