Quick survey for detection, identification and characterization of Acanthamoeba genotypes from some selected soil and water samples in Pakistan
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Department of Medical Laboratory Technology, University of Haripur, Haripur, Khyber Pakhtunkhwa, Pakistan
Department of Environmental Sciences, International Islamic University, Islamabad, Pakistan
Institute of Biomedical and Genetic Engineering, Islamabad, Pakistan
Ann Agric Environ Med. 2015;22(2):227-230
Acanthamoeba is an opportunistic protozoan pathogen which is widely distributed in nature and plays a pivotal role in ecosystem. Acanthamoeba species may cause blinding keratitis and fatal granulomatous encephalitis involving central nervous system. In this study, we investigated the presence of Acanthamoeba in soil and water resources of Pakistan. Here, Acanthamoeba were recovered on non-nutrient agar plate lawn with E.coli and identified by morphological characteristics of the cyst. Furthermore PCR was performed with genus-specific primers followed by direct sequencing of the PCR product for molecular identification. Overall our PCR and sequencing results confirmed pathogenic genotypes including T4 and T15 from both soil and water samples. This is our first report of Acanthamoeba isolation from both soil and water resources of Pakistan which may serve as a potential treat to human health across the country.
The authors express their thanks to Brig. Muhammad Farooq Murawat, Asim Wazir Khan Naizi, Sohail Khan Naizi, Junaid Khan, Abu Turab Khan and Ghazala Shabnum for their tremendous support in the collection and transportation of samples. This work was partially supported by grants from University of Haripur, Khyber Pakhtunkhwa, Pakistan.
Stehr-Green JK, Baily TM, Visvesvara GS. The epidemiology of Acanthamoeba keratitis in the United States. Am J Ophthalmol. 1989; 107: 331–336.
Badenoch PR, Adams M, Coster DJ. Corneal virulence, cytopathic effect on human keratocytes and genetic characterization of Acanthamoeba. Int J Parasitol. 1994; 25: 229–239.
Sriram R, Shoff M, Booton G, Fuerst P, Visvesvara GS. Survival of Acanthamoeba cysts after desiccation for more than 20 years. J Clin Microbiol. 2008; 46: 4045–4048.
Visvesvara GS, Moura H, Schuster FL. Pathogenic and opportunistic free living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Negleria fowleri and Sappinia diploidea. FEMS Immun and Med Microbiol. 2007; 50: 1–26.
Nuprasert W, Putaporntip C, Pariyakanok L, Jongwutiwes S. Identification of a Novel T17 Genotype of Acanthamoeba from Environmental Isolates and T10 Genotype Causing Keratitis in Thailand. J Clin Microbiol. 2010; 48: 4636–4640.
Shoaib HM, Muazzam AG, Mir A, Jung S-Y, Matin A. Evaluation of inhibitory potential of some selective methanolic plants extracts on biological characteristics of Acanthamoeba castellanii using human corneal epithelial cells in vitro. Parasitol Res. 2013; 112 (3): 1179–1188.
Matin A, Jung S-Y. Interaction of Escherichia coli K1 and E. coli K5 with Acanthamoeba castellanii trophozoites and cysts. Korean J of Parasitol. 2011; 49: 349–356.
Tanveer T, Hameed A, Muazzam AG, Jung S-Y, Gul A, Matin A. Isolation and molecular characterization of potentially pathogenic Acanthamoeba genotypes from diverse water resources including household drinking water from Khyber Pakhtunkhwa, Pakistan. Parasitol Res. 2013; 112 (8): 2925–2932.
Lorenzo-Morales J, Monteverde-Miranda CA, Jiménez C, Tejedor ML, Valladares B, Ortega-Rivas A. Evaluation of Acanthamoeba isolates from environmental sources in Tenerife, Canary Islands, Spain. Ann Agric Environ Med. 2005; 12: 233–236.
Matin A, Jeong SR, Faull J, Khan NA. Evaluation of prokaryotic and eukaryotic cells as food source for Balamuthia mandrillaris. Arch of Microbiol. 2006; 186: 261–271.
Booton GC, Kelly DJ, Chu YW, Seal DV, Houang E, Lam DSC, Byers TJ, Fuerst PA. 18S ribosomal DNA typing and tracking of Acanthamoeba species isolates from corneal scrape specimens, contact lens, lens cases, and home water supplies of Acanthamoeba keratitis patients in Hong Kong. J Clin Microbiol. 2002; 40: 1621–1625.
Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans. Clin Microbiol Rev. 2003; 16: 273–307.
Matin A, Ismail M, Mehmood K. Acanthamoeba castellanii. antibody prevalence among diverse tribal Pakistani population. Retrovirology 2012; 9: 47.
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