Occurrence of Mycobacterium spp. in ornamental fish
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Department of Fish Diseases and Biology, Faculty of Veterinary Medicine, University of Life Sciences, Lublin, Poland
Corresponding author
Leszek Guz   

University of Life Sciences, Faculty of Veterinary Medicine, Department of Fish Diseases and Biology, Akademicka 12, 20-950, Lublin, Poland
Ann Agric Environ Med. 2020;27(4):535-539
Introduction and objective:
Fish mycobacteriosis is a chronic granulomatous disease caused by several species of bacteria from the genus Mycobacterium, described as nontuberculous mycobacteria (NTM). The most important species causing fish mycobacterioses are M. chelonae, M. fortuitum, and M. marinum. Mycobacteria infecting fish also include zoonotic pathogens. M. marinum is the cause of most cases of fish-related mycobacterial infection in humans. The disease occurs more frequently in workers in the fishing industry, people whose hobbies involve water activities, and aquarists. The aim of the present study was to examine the occurrence of different species of mycobacteria in freshwater ornamental fish.

Material and methods:
The occurrence of Mycobacterium spp. in freshwater ornamental fish was studied from January 2015 – December 2016. Material isolated from skin scrapings, contents of the digestive tracts, and internal organs of ornamental fish was stained with Ziehl-Neelsen (ZN) and inoculated on Lowenstein-Jensen medium. All isolates found positive by ZN were identified by amplification of the gene encoding the Hsp65 protein. A total of 408 samples obtained from 136 ornamental fish from 36 species were tested.

Using the culture method Mycobacterium was isolated from 69 fish (50.1%) and 99 samples (24.3%). Sequence analysis of gene fragments coding for the Hsp65 protein of 99 isolates revealed occurrence of 13 species of mycobacteria: M. abscessus, M. chelonae, M. fortuitum, M. gordonae, M. marinum, M. mucogenicum, M. neoaurum, M. peregrinum, M. salmoniphilum, M. saopaulense, M. senegalense, M. septicum, and M. szulgai.

The obtained results indicate a significant role of ornamental fish as a source of mycobacteria which are potentially dangerous,especially to humans.

Puk K, Guz L. Occurrence of Mycobacterium spp. in ornamental fish. Ann Agric Environ Med. 2020; 27(4): 535–539. doi: 10.26444/aaem/114913
Gauthier DT, Rhodes MW. Mycobacteriosis in fishes: A review. The Vet J. 2009; 180: 33–47.
Decostere A, Hermans K, Haesebrouck F. Piscine mycobacteriosis: A literature review covering the agent and the disease it causes in fish and humans. Vet Microbiol. 2004; 99: 159–166.
Beran V, Matlova L, Dvorska L, Svastova P, Pavlik I. Distribution of mycobacteria in clinically healthy ornamental fish and their aquarium environment. J Fish Dis. 2006; 29: 383–393.
Guz L, Grądzki Z, Krajewska M, Lipiec M, Zabost A, Augustynowicz-Kopeć E, Zwolska Z, Szulowski K. Occurrence and antimicrobial susceptibility of Mycobacterium peregrinum in ornamental fish. Bull Vet Ins Pulawy 2013; 57: 489–492.
Lescenko P, Matlova L, Dvorska L, Bartos M, Vavra O, Navratil S, Novotny L, Pavlik I. Mycobacterial infection in aquarium fish. Vet Med.(Praha) 2003; 48: 71–78.
Levi MH, Bartell J, Gandolfo L, Smole SC, Costa SF, Weiss LM, Johnson LK, Osterhout G, Herbst LH. Characterization of Mycobacterium montefiorense sp. nov., a novel pathogenic Mycobacterium from moray eels that is related to Mycobacterium triplex. J Clin Microbiol. 2003; 41:2147–2152.
Marumo K, Nakamura H, Tarawa S, Kazumi Y, Kawano R, Shirata C, Taguchi K, Kikuchi T, Nagashima G. Isolation of novel mycobacteria contaminating an aquarium fish tank in a Japanese university hospital. J Appl Microbiol. 2010; 109: 558–566.
Nogueira CL, Whipps CM, Matsumoto CK, Chimara E, Droz S, Tortoli E, Freitas DD, Cnockaert M, Palomino JC, Martin A, Vandamme P, Leão SC. Mycobacterium saopaulense sp. nov., a rapidly growing mycobacterium closely related to members of the Mycobacterium chelonae–Mycobacterium abscessus group. Int J Syst Evol Microbiol. 2015; 65: 4403–4409.
Pate M, Jencic V, Zolnir-Dovc M, Ocepek M. Detection of mycobacteria in aquarium fish in Slovenia by culture and molecular methods. Dis Aquat Org. 2005; 64: 29–35.
Rhodes MW, Kator H, Kotob S, van Berkum P, Kaattari I, Vogelbein W, Quinn F, Floyd MM, Butler WR, Ottinger CA. Mycobacterium shottsii sp. nov., a slowly growing species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int J Syst Evol Microbiol. 2003; 53: 421–424.
Whipps CM, Dougan ST, Kent ML. Mycobacterium haemophilum infections of zebrafish ( Danio rerio) in research facilities. FEMS Microbiol Lett. 2007; 270: 21–26.
Zanoni RG, Florio D, Fioravanti ML, Rossi M, Prearo M. Occurrence of Mycobacterium spp. in ornamental fish in Italy. J Fish Dis. 2008; 31: 433–441.
Zerihun MA, Berg V, Lyche JL, Colquhoun DJ, Poppe TT. Mycobacterium salmoniphilum infection in burbot Lota lota. Dis Aquat Org. 2011; 95: 57–64.
Strike TB, Feltrer Y, Flach E, Macgregor SK, Guillaume S. Investigation and management of an outbreak of multispecies mycobacteriosis in Australian lungfish ( Neoceratodus fosteri) including the use of triple antibiotic treatment. J Fish Dis. 2017; 40: 557–570.
Bhatty MA, Turner DP, Chamberlain ST. Mycobacterium marinum hand infection: case reports and review of literature. Brit J Plast Surg. 2000; 53: 161–165.
Dodiuk-Gad R, Dyachenko P, Ziv M, Shani-Adir A, Oren Y, Mendelovici S, Shafer J, Chazan B, Raz R, Keness Y, Rozenman D. Nontuberculous mycobacterial infections of the skin: a retrospective study of 25 cases. J Am Acad Dermatol. 2007; 57: 413–420.
Talavlikar R, Carson J, Meatherill B, Desai S, Sharma M, Shandro C, Tyrrell GJ, Kuhn S. Mycobacterium senegalense tissue infection in a child after fish tank exposure. Can J Infect Dis Med Microbiol. 2011; 22: 101–103.
Shukla S, Shukla SK, Sharma R, Kumar A. Identification of Mycobacterium species from apparently healthy freshwater aquarium fish using partial sequencing and PCR-RFLP analysis of heat shock protein (hsp65) gene. J Appl Ichthyol. 2014; 30: 513–520.
Wallace RJ, Jr, Iakhiaeva E, Williams MD, Brown-Elliott BA, Vasireddy S, Vasireddy R, Lande L, Peterson DD, Sawicki J, Kwait R, Tichenor WS, Turenne C, Falkinham JO. Absence of Mycobacterium intracellulare and presence of Mycobacterium chimaera in household water and biofilm samples of patients in the United States with Mycobacterium avium complex respiratory disease. J Clin Microbiol. 2013; 51: 1747–1752.
Kim SH, Shin JH. Identification of nontuberculous mycobacteria using multilocous sequence analysis of 16S rRNA, hsp65, and rpoB. J Clin Lab Anal. 2018; 32: e22184.
Telenti A, Marchesi F, Balz M, Bally F, Bottger EC, Bodmer T. Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis. J Clin Microbiol. 1993; 31: 175–178.
Novotny L, Dvorska L, Lorencova A, Beran V, Pavlik I. Fish: A potential source of bacterial pathogens for human beings. Vet Med. (Praha) 2004; 9: 343–358.
Puk K, Banach T, Wawrzyniak A, Adaszek Ł, Ziętek J, Winiarczyk S, Guz L. Detection of Mycobacterium marinum, M. peregrinum, M. fortuitum and M. abscessus in aquarium fish. J Fish Dis. 2018; 41: 153–156.
Antychowicz J, Lipiec M, Matusiewicz J. Infection of African catfish (Clarias gariepinus) in an intensive culture facility Mycobacterium marinum. Bull Eur Ass Fish Pathol. 2003; 23: 60–66.
Hongslo T, Jansson E. Occurrence of different species of mycobacteria in aquarium fish from Swedish pet-shops. Bull Eur Ass Fish Pathol. 2014; 34: 78–86.
Pollock HM, Wieman EJ. Smear results in the diagnosis of mycobacterioses using blue light fluorescence microscopy. J Clin Microbiol. 1977; 5: 329–331.
Wright PW, Wallace RJ Jr., Wright NW, Brown BA, Griffith DE. Sensitivity of fluorochrome microscopy for detection of Mycobacterium tuberculosis versus nontuberculous mycobacteria. J Clin Microbiol. 1998; 36: 1046–1049.
Smith KF, Schmidt V, Rosen GE, Amaral-Zettler L. Microbial Diversity and Potential Pathogens in Ornamental Fish Aquarium Water. PLOS ONE 2012; 7:e39971.
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