New aspects of the infection mechanisms of B. anthracis
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Biological Threats Identification and Coutermeasure Center of the Military Institute of Hygiene and Epidemiology, Puławy, Poland
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Dorota Żakowska   

Biological Threats Identification and Coutermeasure Center of the Military Institute of Hygiene and Epidemiology, Puławy, Poland
Ann Agric Environ Med. 2012;19(4):613-618
Articles concerning new aspects of B. anthracis mechanisms of infection were reviewed. It was found, that the hair follicle plays an important role in the spore germination process. The hair follicle represent an important portal of entry in the course of the cutaneous form of disease infections. After mouse exposition to aerosol of spores prepared from B. anthracis strains, an increase in the level of TNF-α cytokines was observed. The TNF-α cytokines were produced after intrusion into the host by the microorganism. This process may play a significant role in the induced migration of infected cells APCs (Antigen Presenting Cells) via chemotactic signals to the lymph nodes. It was explained that IgG, which binds to the spore surface, activates the adaptive immune system response. As a result, the release C3b opsonin from the spore surface, and mediating of C3 protein fragments of B. anthracis spores phagocytosis by human macrophages, was observed. The genes coding germination spores protein in mutant strains of B. anthracis MIGD was a crucial discovery. According to this, it could be assumed that the activity of B. anthracis spores germination process is dependent upon the sleB, cwlJ1 and cwlJ2 genes, which code the GSLEs lithic enzymes. It was also discovered that the specific antibody for PA20, which binds to the PA20 antigenic determinant, are able to block further PA83 proteolytic ssion on the surface of cells. This process neutralized PA functions and weakened the activity of free PA20, which is produced during the PA83 enzyme ssion process. Interaction between PA63 monomer and LF may be helpful in the PA63 oligomerization and grouping process, and the creation of LF/PA63 complexes may be a part of an alternative process of assembling the anthrax toxin on the surface of cells. It was found that actin-dependent endocytosis plays an important role in the PA heptamerisation process and leads to blocking the toxin activity. Chaperones, a protein derived from host cells, may be helpful in ATP and cytosolic factors translocation, and in this way increase the translocation of diphteria toxin A domein (DTA) and substrate of fusion protein LFN-DTA.
Barua S, McKevitt M, DeGiusti K, Hamm EE, Larabee J, Shakir S, et al. The mechanism of Bacillus anthracis intracellular germination requires multiple and highly diverse genetic loci. Infect Immun. 2009; 77: 23-31.
Goldman DL, Casadevall A. Anthrax-associated shock. Front Biosci. 2008; 13: 4009-4014.
Bischof TS, Hahn BL, Sohnle PG. Characteristics of spore germination in a mouse model of cutaneous anthrax. J Infect Dis. 2007; 195: 888-94.
Hahn BL, Sharma S, Sohnle PG. Analysis of epidermal entry in experimental cutaneous Bacillus anthracis infections in mice. J Lab Clin Med. 2005; 146(2): 95-101.
Gut IM, Tamilselvam B, Prouty AM, Stojkovic B, Czeschin S, van der Donk WA, et al. Bacillus anthracis spore interactions with mammalian cells: relationship between germination state and the outcome of in vitro infections. BMC Microbiology 2011; 11: 46.
Loving CL, Kennett M, Lee GM, Grippe VK, Merkel TJ. Murine aerosol challenge model of anthrax. Infect Immun. 2007; 75(6): 2689-2698.
Premanandan C, Storozuk CA, Clay CD, Lairmore MD, Schlesinger LS, Phipps AJ. Complement protein C3 binding to Bacillus anthracis spores enhances phagocytosis by human macrophages. Microb Pathog. 2009; 46: 306-314.
Oliva C, Turnbough CL Jr, Kearney JF. CD14-Mac-1 interactions in Bacillus anthracis spore internalization by macrophages. PNAS 2009; 106(33): 13957-13962.
Moayeri M, Leppla SH. e roles of anthrax toxin in pathogenesis. Curr Opin Microbiol. 2004; 7: 19-24.
Giebel JD, Carr KA, Anderson EC, Hanna PC. The germination-specific lytic enzymes SleB, CwlJ1, and Cw1J2 each contribute to Bacillus anthracis spore germination and virulence. J Biotechnol. 2009; 191(18): 5569-5576.
Setlow P. Spore germination. Curr Opin Microbiol. 2003; 6: 550-556.
Heffron JD, Lambert EA, Sherry N, Popham DL. Contributions of four cortex lytic enzymes to germination of Bacillus anthracis spores. J Biotechnol. 2010; 192(3): 763-770.
Mourez M. Anthrax toxins. Rev Physiol Biochem Pharmacol. 2004; 152: 135-164.
Abrami L, Bischo erger M, Kunz B, Groux R, van der Goot FG. Endocytosis of the anthrax toxin is mediated by clathrin, actin and unconventional adaptors. PLoS Pathogens. 2010; 6(3): e1000792.
Żakowska D, Bartoszcze M, Niemcewicz M, Bielawska-Drózd A. Nowe możliwości hamowania infekcji B. anthracis. Med Wet. 2011; 67(10): 665-668.
Hammamieh R, Ribot WJ, Abshire TG, Jett M, Ezzell J. Activity of the Bacillus anthracis 20 kDa protective antigen component. BMC Infect Dis. 2008; 8: 124.
Chvyrkova I, Zhang XC, Terzyan S. Lethal factor of anthrax toxin binds monomeric form of protective antigen. Biochem Biophys Res Commun. 2007; 360(3): 690-695.
Reason DC, Ullal A, Liberato J, Sun J, Keitel W, Zhou J. Domain specificity of the human antibody response to Bacillus anthracis protective antigen. Vaccine 2008; 26: 4041-4047.
Żakowska D, Kocik J, Bartoszcze M. Wybrane kierunki badań nad szczepionkami przeciwko wąglikowi. Przegl Epidemiol. 2009; 63: 505-512.
Kintzer AF, Sterling HJ, Tang II, Williams ER, Krantz BA. Anthrax toxin receptor drives protective antigen oligomerization and stabilizes the heptameric and octameric oligomer by a similar mechanism. PloS ONE 2010; 5(11): e 13888.
Katayama H, Wang J, Tama F, Chollet L, Gogol EP, Collier RJ, et al. Three-dimensional structure of the anthrax toxin pore inserted into lipid nanodiscs and lipid vesicles. PNAS 2010; 107(8): 3453-3457.
Janowiak BE, Fischer A, Collier RJ. Effects of introducing a single charged residue into the phenylalanine clamp of multimeric anthrax protective antigen. J Biol Chem. 2010; 285(11): 8130-8137.
Thoren KL, Krantz BA. The unfolding story of anthrax toxin translocation. Mol Microbiol. 2011; 80(3): 588-595.
Cui X, Moayeri M, Li Y, Li X, Haley M, Fitz Y, et al. Lethality during continuous anthrax lethal toxin infusion is associated with circulatory shock but not inflammatory cytokine or nitric oxide release in rats. Am J Physiol Regul Integr Comp Physiol. 2004; 286: 699-709.
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