RESEARCH PAPER
Isolation and amplification by polymerase chain reaction DNA of Babesia microti and Babesia divergens in ticks in Poland.
 
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Department of Genetics, Faculty of Biology, Szczecin University, Szczecin, Poland
 
 
Corresponding author
Bogumiła Skotarczak   

Department of Genetics, Faculty of Biology, Szczecin University, Szczecin, Piastów 40 B, 71-065 Szczecin, Poland
 
 
Ann Agric Environ Med. 2001;8(2):187–189
 
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ABSTRACT
Babesia microti and B. divergens, the etiological agents of human babesiosis, are transmitted by the bite of Ixodes ricinus. The purpose of this study was differentiation of those two species in ticks collected in urban woods in the city Szczecin (north-western Poland). The prevalence of the DNA of Babesia were investigated by PCR amplification with primers to the fragment from a gene encoding the nuclear small-subunit ribosomal RNA (SS-rDNA). We examined a total of 533 specimens of Ixodes ricinus. The mean infection rate was 16.3%. Our results indicate that a B. microti and B. divergens-specific PCR test may provide a sensitive tool also for the laboratory diagnosis of human babesiosis.
eISSN:1898-2263
ISSN:1232-1966