RESEARCH PAPER
Biological and environemntal conditionings for a sperm DNA fragmentation
| 1 | Department for Health Problems of Ageing, Institute of Rural Health, Lublin, Poland |
| 2 | College of Public Health, Zielona Góra, Poland |
| 3 | Laboratory of Diagnostic Procedures. Faculty of Nursing and Health Sciences, Medical University, Lublin, Poland |
Ann Agric Environ Med. 2013;20(4):865–868
KEYWORDS
ABSTRACT
The objective of the presented study was determination of the effect of selected agents on sperm DNA fragmentation – superoxide dismutase in seminal plasma, the patients’ age, and burdening with the tobacco smoking habit. An attempt was also undertaken to evaluate the effect of DNA fragmentation on the effectiveness of infertility treatment.
The study covered 186 men who received treatment due to infertility. The database and statistical analyses were performed using computer software STATISTICA 7.1.
A relationship was observed between sperm DNA fragmentation and superoxide dismutase activity, the higher the SOD activity, the lower the percentage of sperm fragmentation (rs=−0.324; P=0.000; r = –0.2110). A statistical relationship was found between sperm DNA fragmentation and the percentage of pregnancies obtained during the first year of treatment – patients with the lower DFI more frequently became fathers during the first year of trying, compared to the remainder (t=2.51; P=0.013). A statistically significant relationship was confirmed (rs=−0.370; P=0.000) consisting in an increase in the DFI with respondents’ age. No significant differences were noted between the DFI and the tobacco smoking habit (Chi2=0.29; P=0.926).
The percentage of sperm DNA fragmentation was inversely proportional to superoxide dismutase activity in seminal plasma.
DNA fragmentation becomes intensified with patients’ age. Cigarette smoking has no effect on sperm DNA fragmentation.
DNA fragmentation exerts an effect on the effectiveness of infertility treatment.
REFERENCES (24)
1.
Aktan G, Dogru-Abbasoglu S, Kucukgergin C, Kadioglu A, OzdemirlerErata G, Koçak-Toker N. Mystery of idiopathic male infertility: is oxidative stress an actual risk? Fertil Steril. 2013; 99(5): 1211–5.
2.
Bungum M, Bungum L, Giwercman A. Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility. Asian J Androl. 2011; 13: 69–75.
3.
Erenpreiss J, Spano M, Erenpreisa J, Bungum M, Giwercman A. Sperm chromatin structure and male fertility: biological and clinical aspects. Asian J Androl. 2006; 8: 11–29.
4.
Dobrzyńska MM, Tyrkiel E, Derezińska E, Ludwicki J. Is concentration and motility of male gametes related to DNA damage measured by comet assay? Ann Agric Environ Med. 2010; 17: 73–7.
5.
Atig F, Kerkeni A, Saad A, Ajina MJ. Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men. J Assist Reprod Genet. 2013. doi: 10.1007/s10815–013–9936-x.
6.
Tartibian B, Maleki BH. Correlation between seminal oxidative stress biomarkers and antioxidants with sperm DNA damage in elite athletes and recreationally active men. Clin J Sport Med. 2012; 22: 132–9.
7.
Omran HM, Bakhiet M, Dashti MG. DNA integrity is a critical molecular indicator for the assessment of male infertility. Mol Med Rep. 2013; 7(5): 1631–5.
8.
Shiva M, Gautam AK, Verma Y, Shivgotra V, Doshi H, Kumar S. Association between sperm quality, oxidative stress, and seminal antioxidant activity. Clin Biochem. 2011; 44: 319–24.
9.
Shamsi MB, Venkatesh S, Kumar R, Gupta NP, Malhotra N, Singh N, Mittal S, Arora S, Arya DS, Talwar P, Sharma RK, Dada R. Antioxidant levels in blood and seminal plasma and their impact on sperm parameters in infertile men. Indian J Biochem Biophys. 2010; 47: 38–43.
10.
Murawski M, Saczko J, Marcinkowska A, Chwiłkowska A, Gryboś M, Banaś T. Evaluation of superoxide dismutase activity and its impact on semen quality parameters of infertile men. Folia Histoch Cytobiol. 2007; 45 (Supp. 1): 123–126.
11.
Zelen I, Mitrović M, Jurisic-Skevin A, Arsenijević S. Activity of superoxide dismutase and catalase and content of malondialdehyde in seminal plasma of infertile patients. Med Pregl. 2010; 63: 624–9.
12.
Ménézo Y. Antioxidants to reduce sperm DNA fragmentation: an unexpected adverse effect. Reprod BioMed. Online 2007; 14: 418–421.
13.
Acosta SA, Vargas ES, Cuya VM, Gonzalez RJ, Gutierrez SR. Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation. Theriogenology 2013; 79: 842–6.
14.
Nagy S, Johannisson A, Wahlsteen T, Ijas R, Andersson M, RodriguezMartinez H. Sperm chromatin structure and sperm morphology: Their association with fertility in AI-dairy Ayrshire sires. Theriogenology 2013; 79(8): 1153–61.
15.
Erenpreiss J, Elzanaty S, Giwercman A. Sperm DNA damage in men from infertile couples. Asian J Androl. 2008; 10: 786–90.
16.
Borini A, Tarozzi N, Bizzaro D, Bonu MA, Fava L, Coticchio G. Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART. Hum Reprod. 2006; 21: 2876–2881.
17.
Kłuciński P, Wójcik A, Grabowska-Bochenek R, Mazur B, Hrycek A, Hrycek A, Cieślik P, Martirosian G. Erythrocyte antioxidant parameters in workers occupationally exposed to low levels of ionizing radiation. Ann Agric Environ Med. 2008; 15: 9–12.
18.
Li WW, Li N, Wu QY, Xia XY, Cui YX, Yao Q. Cigarette smoking affects sperm plasma membrane integrity. Zhonghua Nan Ke Xue 2012; 18: 1093–6.
19.
Panasiuk L, Mierzecki A, Wdowiak L, Paprzycki P, Lukas W, GodyckiCwirko M. Prevalence of cigarette smoking among adult population in eastern Poland. Ann Agric Environ Med. 2010; 17: 133–8.
20.
Wu JQ, Li YY, Gao ES, Rong F, Zhou JS, Ji M, Ding D. The influence of smoking on the routine parameters of semen quality. Zhonghua Liu Xing Bing Xue Za Zhi 2012; 33: 1228–32.
21.
Zhu Z, Xu W, Dai J, Chen X, Zhao X, Fang P, Yang F, Tang M, Wang Z, Wang L, Ma D, Qaio Z. The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis. Int J Biochem Cell Biol. 2013; 45: 571–82.
22.
Encisio M, Alfarawati S, Wells D. Increased numbers of DNA-damaged spermatozoa in samples presenting an elevated rate of numerical chromosome abnormalities. Hum Reprod. 2013; 28(6): 1707–15.
23.
Khadem N, Poorhoseyni A, Jalali M, Akbary A, Heydari ST. Sperm DNA fragmentation in couples with unexplained recurrent spontaneous abortions. Andrologia 2012. doi: 10.1111/and.12056.
24.
Pan F, Xu ZP, Shi L, He Z, Gong CK, Dai YT, Pan LJ. Seminal parameters at different times of reanalysis of the normal semen samples detected in initial examination and their correlation with sperm DNA damage. Zhonghua Nan Ke Xue 2013; 19: 63–7.
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