RESEARCH PAPER
Superoxide dismutase and glutathione peroxidase activity in pregnancy complicated by diabetes
 
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1
Diagnostic Techniques Unit, Faculty of Health Sciences, Medical University in Lublin, Poland
2
Luxmed Medical Center, Lublin, Poland
3
Department for Health Problems of Ageing, Institute of Rural Health, Lublin, Poland
 
Ann Agric Environ Med. 2015;22(2):297–300
KEYWORDS
ABSTRACT
The objective of the study was determination of the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in blood and placental tissues of pregnant women with pregnancy complicated by diabetes, pregnant women with physiological pregnancy, and non-pregnant women, as well as a comparative analysis of blood and placental tissue parameters in the groups of women examined. The material for the study was blood and placental tissue from 50 pregnant women who received treatment due to insulin-dependent diabetes (PD). For the control group, 50 pregnant women without diabetes (HP), and 30 non-pregnant women (NP) were selected. SOD activity in erythrocytes was evaluated by the method of spectrophotometry with the use of RANSOD kit (RANDOX Laboratories Ltd., UK). The activity of GPx activity in erythrocytes was determined according to the method by Paglia and Valentine using RANSEL kit (RANDOX Laboratories Ltd). The results were subject to statistical analysis. Insulin-dependent diabetes in pregnancy affects the activity of anti-oxidative enzymes. In the blood of women with pregnancy complicated by diabetes, the activity of anti-oxidative enzymes – SOD and GPx is higher than in the blood of women with physiological pregnancy and the control group. In the placental tissue from pregnancy complicated by diabetes, the activity of SOD significantly decreases, while the activity of GPx increases, compared to women in physiological pregnancy.
 
REFERENCES (25)
1.
Hartling L, Dryden DM, Guthrie A, Muise M, Vandermeer B, Donovan L. Benefits and Harms of Treating Gestational Diabetes Mellitus: A Systematic Review and Meta-analysis for the U.S. Preventive Services Task Force and the National Institutes of Health Office of Medical Applications of Research. Ann Intern Med. 2013; 159(2):123–129. doi: 10.7326/0003–4819–159–2–201307160–00661.
 
2.
Gajewska M, Gebska-Kuczerowska A, Gorynski P, Wysocki MJ. Analyses of hospitalization of diabetes mellitus patients in Poland by gender, age and place of residence. Ann Agric Environ Med. 2013; 20(1): 61–67.
 
3.
Arora D, Arora R, Sangthong S, Leelaporn W, Sangratanathongkhai J. Universal screening of gestational diabetes mellitus: prevalence and diagnostic value of clinical risk factors. J Med Assoc Thai. 2013; 96(3): 266–271.
 
4.
Lehnen H, Zechner U, Haaf T. Epigenetics of gestational diabetes mellitus and offspring health: the time for action is in early stages of life. Mol Hum Reprod. 2013; 19(7): 415–422.
 
5.
Cyganek K, Klupa T, Szopa M, Katra B, Małecki MT. Medical care of pregnant women with type 1 diabetes: current guidelines and clinical practice. Pol Arch Med Wewn. 2013; 123(1–2): 59–65.
 
6.
Hawdon JM. Babies born after diabetes in pregnancy: what are the short- and long-term risks and how can we minimise them? Best Pract Res Clin Obstet Gynaecol. 2011; 25: 91–104.
 
7.
Desai M, Beall M, Ross MG. Developmental origins of obesity: programmed adipogenesis. Curr Diab Rep. 2013; 13: 27–33.
 
8.
Moore TR. Fetal exposure to gestational diabetes contributes to subsequent adult metabolic syndrome. Am J Obstet Gynecol. 2010; 202: 643–649.
 
9.
Drake AJ, Reynolds RM. Impact of maternal obesity on offspring obesity and cardiometabolic disease risk. Reproduction 2010;140: 387–398.
 
10.
Ball S. Antyoksydanty w medycynie i zdrowiu człowieka. Medyk, Warszawa 2001 (in Polish).
 
11.
Clapés S, Fernández T, Suárez G. Oxidative Stress and Birth Defects in Infants of Women with Pregestational Diabetes. MEDIPD Review. 2013; 15(1): 37–40.
 
12.
Leal CAM, Schetinger MRC, Leal DBR. Oxidative stress and antioxidant defenses in pregnant women. Redox Report 2011; 16(6): 230–236.
 
13.
Szelachowska M. Rola stresu oksydacyjnego w procesach chorobowych. Terap. 2002; 5: 19–22 (in Polish).
 
14.
Twardowska-Saucha K, Grzeszczak W, Lacka B, Froehlich J, Krywult D. Lipid peroxidation, antioxidant enzyme activity and trace element concentration in II and III trimester of pregnancy in pregnant women with diabetes. Pol Arch Med Wewn. 1994; 92(4): 313–321.
 
15.
Fridovitch I, McCord JM. Superoxide dismutase an enzimic function for erythrocuprein (hemocuprein). J Biol Chem. 1969; 244: 6049–6055.
 
16.
Paglia DE, Valentine WN, Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967; 70: 158–169.
 
17.
Agarwal A, Gupta S, Sikka S. The role of free radicals and antioxidants In reproduction. Curr Opin Obstem Gynecol. 2006; 18: 325–332.
 
18.
Hong J, Park EA, Kim YJ, Lee HY, Park BH, Ha EH, Kong KA, Park H. Association of antioxidant vitamins and oxidative stress levels in pregnancy with infant growth during the first year of life. Publ Healt Nutr. 2007; 7: 1–8.
 
19.
Mier Cabrera J, Genera-Gracia M, De la Jara-Diaz J, Perichart-Perera O, Vadillo-Ortega F, Hernandez-Gurrero C. Effect of vitamins C and E supplementation on peripheral oxidative stress markers and pregnancy rate in woman with endometriosis. Int J Gynecol Obstet. 2007; 13: 1–10.
 
20.
Walsh SW. Obesity: a risk factor for preeclampsia. Trend Endocrinol Metab. 2007; 18: 365–370.
 
21.
Nishikava T, Edelstein D, Du XL, Yamagishi SL, Matsumura T, Keneda Y, Yoreks MA, Beebe D, Oates PJ, Hammers HP, Giardino L, Brownlee M. Normalizing mitochondrial superoxide production bloks three pathways of hyperglycemic damage. Natur. 2000; 404: 787–790.
 
22.
Książek K, Wiśniewska J. Udział glukozy i reaktywnych form tlenu w powstawaniu naczyniowych powikłań cukrzycy. Przegl Lek. 2001; 58(10): 915–918 (in Polish).
 
23.
Zozulińska D. The influence of IDDM duration on superoxide anion and hydrogen peroxide production by polymorphonuclear neutrophils. Diab Res Clin Pract. 1996; 33: 139–144.
 
24.
Takehara Y, Yamaoka K, Hiraki Y, Yoshioka T, Utsumi K. Protection against alloxan diabetes by low-dose 60Co gamma irradiation before alloxan administration. Physiol Chem Phys Med. 1995; 27(3): 149–159.
 
25.
Alcolea MP, Llado I, Garcia-Palmer FJ, Gianotti M. Responses of mitochondrial biogenesis and function to maternal diabetes in rat embryo during the placentation period. Am J Physiol Endocrine Metab. 2007; 293: 636–644.
 
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