RESEARCH PAPER
Potential sources of infection with selected zoonotic agents in the veterinary work environment – pilot studies
1
Department of Health Biohazards and Parasitology, Institute of Rural Health, Lublin, Poland
2
Department of Parasitology, National Veterinary Research Institute, Pulawy, Poland
3
Department of Parasitology and Invasive Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin,
Poland
4
Department of Epidemiology and Biostatistics, Institute of Rural Health, Lublin, Poland
Corresponding author
Angelina Wójcik-Fatla
Institute of Rural Health, Department of Health Biohazards and Parasitology, Jaczewskiego 2, 20-090, Lublin, Poland
Institute of Rural Health, Department of Health Biohazards and Parasitology, Jaczewskiego 2, 20-090, Lublin, Poland
Ann Agric Environ Med. 2020;27(1):146-150
KEYWORDS
environmentCoxiella burnetiiToxoplasma gondiiveterinariansLeptospira sppCryptosporidium sppGiardia duodenalis
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Prevention of occupational diseases in agriculture, forestry, food industry and wood industryState of the health of rural communities depending on various factors: social factors, accessibility of medical care, etc.
ABSTRACT
Introduction and Objective:
The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii.
Material and methods:
A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples.
Results:
DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces.
Conclusions:
The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.
The problem of occupational biohazards is very important, especially in the field of agriculture and in human and veterinary medicine. The aim of the study was to determine the potential sources of infection in veterinary professionals with selected zoonotic agents, including: Toxoplasma gondii, Giardia duodenalis, Leptospira spp., Cryptosporidium spp. and Coxiella burnetii.
Material and methods:
A total of 50 air samples from barns, piggeries and veterinary surgeries were examined for the presence of Leptospira spp. and C. burnetii DNA. Serum samples of 86 pigs and 80 cows were tested for the presence of antibodies to Leptospira spp. and to phase I and II C. burnetii antigens. Serum of 70 cats were tested for the presence of antibodies to T. gondii and 65 samples of cat faeces for the presence of T. gondii oocysts. The presence of G. duodenalis and Cryptosporidium spp. were examined in 50 of dog faeces and 50 of bovine faeces samples.
Results:
DNA of Leptospira spp. was detected in 2 air samples from the piggeries (4%). C. burnetii DNA was not found in any sample. Anti-Leptospira spp. antibodies were detected in 51 (59.3%) of examined pigs. Neither anti-Leptospira spp. nor anti-C. burnetii antibodies were found among samples of bovine serum. Anti-T. gondii antibodies was found in 52 cat serum samples (74.3%). Among samples of cat faeces, no T. gondii oocysts were detected. In one sample of cattle stool (2%), G. duodenalis was detected and in another (2%) – Cryptosporidium spp. G. duodenalis was detected in 7 samples (14%) and Cryptosporidium spp. in 2 samples (2%) of dog faeces.
Conclusions:
The results of this study demonstrate the potential risk of infection with Leptospira spp. in veterinarians working with pigs. Veterinarians could be also be at risk of infection with T. gondii and G. duodenalis.
FUNDING
This study has been based on the results of Phase IV of
the National Programme for the Improvement of Safety
and Working Conditions (2017-2019) financed by the
Ministry of Science and Higher Education/the National
Centre for Research and Development and coordinated by the
Central Institute for Labour Protection–National Research
Institute (agreement No. TP-54/2017/PW-PB, Project No.
II.N.22).
REFERENCES (42)
1.
Dutkiewicz J, Cisak E, Sroka J, Wójcik-Fatla A, Zając V. Biological agents as occupational hazards – selected issues. Ann Agric Environ Med. 2011; 18(2): 286–293.
2.
Baer R, Turnberg W, Yu D, Wohrle R. Leptospirosis in a small animal veterinarian: reminder to follow standardized infection control procedures. Zoonoses Public Health. 2010; 57(4): 281–284.
3.
Costa F, Hagan JE, Calcagno J, Kane M, Torgerson P, Martinez-Silveira MS, et al. Global Morbidity and Mortality of Leptospirosis: A Systematic Review. PLoS Negl Trop Dis. 2015; 9(9): e0003898.
4.
Guernier V, Goarant C, Benschop J, Lau CL. A systematic review of human and animal leptospirosis in the Pacific Islands reveals pathogen and reservoir diversity. PLoS Negl Trop Dis. 2018; 14:12(5): e0006503.
5.
Dubey JP. The history of Toxoplasma gondii-the first 100 years. J Eukaryot Microbiol. 2008; 55(6): 467–475.
6.
Munhoz AD, Souza MA, Costa SCL, Freitas JS, Silva AND, Lacerda LC, et al. Factors associated with the distribution of natural Toxoplasma gondii infection among equids in Northeastern Brazil. Rev Bras Parasitol Vet. 2019; 28(2): 283–290.
7.
Alves BF, Oliveira S, Soares HS, Pena HFJ, Conte-Junior CA, Gennari SM. Isolation of viable Toxoplasma gondii from organs and Brazilian commercial meat cuts of experimentally infected pigs. Parasitol Res. 2019; 118(4): 1331–1335.
8.
Miura AC, de Barros LD, Ferreira FP, Neto JMF, Sicupira Franco PML, et al. Genotyping of Toxoplasma gondii isolated from pigs for human consumption. Parasitol Res. 2019; 118(5): 1593–1599.
9.
Sroka J, Bilska-Zając E, Wójcik-Fatla A, Zając V, Dutkiewicz J, Karamon J, Piotrowska W, Cencek T. Detection and molecular characteristics of Toxoplasma gondii DNA in retail raw meat products in Poland. Foodborne Pathog Dis. 2019; 16(3): 195–204.
10.
Galvani AT, Christ APG, Padula JA, Barbosa MRF, de Araújo RS, Sato MIZ, et al. Real-time PCR detection of Toxoplasma gondii in surface water samples in São Paulo, Brazil. Parasitol Res. 2019; 118(2): 631–640.
11.
Bezerra M, Kim PC, Moraes ÉP, Sá SG, Albuquerque PP, Silva JG, et al. Detection of Toxoplasma gondii in the milk of naturally infected goats in the Northeast of Brazil. Transbound Emerg Dis. 2015: 62(4): 421–424.
12.
Sroka J, Kusyk P, Bilska-Zając E, Karamon J, Dutkiewicz J, Wójcik-Fatla A, Zając V, Stojecki K, Różycki M, Cencek T. Seroprevalence of Toxoplasma gondii infection in goats from the south-west region of Poland and the detection of T. gondii DNA in goat milk. Folia Parasitol. 2017; 64: 023.
13.
Xiao L, Feng Y. Molecular epidemiologic tools for waterborne pathogens Cryptosporidium spp. and Giardia duodenalis. Food Waterborne Parasitol. 2017; 8–9: 14–32.
14.
Ma L, Zhang X, Jian Y, Li X, Wang G, Hu Y, et al. Detection of Cryptosporidium and Giardia in the slaughterhouse sewage and river waters of the Qinghai Tibetan plateau area (QTPA), China. Parasitol Res. 2019; 118(7): 2041–2051.
15.
Holzhausen I, Lendner M, Göhring F, Steinhöfel I, Daugschies A. Distribution of Cryptosporidium parvum gp60 subtypes in calf herds of Saxony, Germany. Parasitol Res. 2019; 118(5): 1549–1558.
16.
Lombardelli JA, Tomazic ML, Schnittger L, Tiranti KI. Prevalence of Cryptosporidium parvum in dairy calves and GP60 subtyping of diarrheic calves in central Argentina. Parasitol Res. 2019; 118(7): 2079–2086.
17.
Stojecki K, Sroka J, Cencek T, Dutkiewicz J. Epidemiological survey in Łęczyńsko-Włodawskie Lake District of eastern Poland reveals new evidence of zoonotic potential of Giardia intestinalis. Ann Agric Environ Med. 2015b;22(4):594–8.
18.
Zhang HJ, Song JK, Wu XM, Li YH, Wang Y, Lin Q, et al. First report of Giardia duodenalis genotypes in Zangxiang pigs from China. Parasitol Res. 2019; 118(7): 2305–2310.
19.
Abdelkadir K, Palomar AM, Portillo A, Oteo JA, Ait-Oudhia K, Khelef D. Presence of Rickettsia aeschlimannii, ‘Candidatus Rickettsia barbariae’ and Coxiella burnetii in ticks from livestock in Northwestern Algeria. Ticks Tick Borne Dis. 2019; 10(4): 924–928.
20.
Porter SR, Czaplicki G, Mainil J, Guattéo R, Saegerman C. Q Fever: current state of knowledge and perspectives of research of a neglected zoonosis. Int J Microbiol. 2011; 2011: 248418.
21.
Barandika JF, Alvarez-Alonso R, Jado I, Hurtado A, García-Pérez AL. Viable Coxiella burnetii in hard cheeses made with unpasteurized milk. Int J Food Microbiol. 2019; 303: 42–45.
22.
Wójcik-Fatla A, Sroka J, Zając V, Zwoliński J, Sawczyn-Domańska A, Kloc A, et al. Study on Toxoplasma gondii, Leptospira spp., Coxiella burnetii and Echinococcus granulosus infection in veterinarians from Poland. J Vet Res. 2018; 62(4): 477–483.
23.
Rolain JM, Raoult D. Molecular detection of Coxiella burnetii in blood and sera during Q fever. QJM. 2005; 98(8): 615–617.
24.
Subramanian G, Sekeyova Z, Raoult D, Mediannikov O. Multiple tick-associated bacteria in Ixodes ricinus from Slovakia. Ticks Tick Borne Dis. 2012; 3(5–6): 406–410.
25.
Amutha R, Chaudhury P, Garg AP, Vasan P, Cheema PS, Srivastava SK. Cloning and sequence analysis of the gene encoding LipL32 of Leptospira interrogans serovar Sejroe. Vet Res Commun. 2007; 31(5): 513–519.
26.
Wójcik-Fatla A, Zając V, Wasiński B, Sroka J, Cisak E, Sawczyn A, et al. Occurrence of Leptospira DNA in water and soil samples collected in eastern Poland. Ann Agric Environ Med. 2014; 21(4): 730–732.
27.
Sroka J, Karamon J, Dutkiewicz J, Wójcik-Fatla A, Cencek T. Optimization of flotation, DNA extraction and PCR methods for detection of Toxoplasma gondii oocysts in cat faeces. Ann Agric Environ Med. 2018; 25(4): 680–685.
28.
Grigg ME, Boothroyd JC. Rapid identification of virulent type I strains of the protozoan pathogen Toxoplasma gondii by PCR restriction fragment length polymorphism analysis at the B1 gene. J Clin Microbiol. 2001; 39(1): 398–400.
29.
Wynwood SJ, Graham GC, Weier SL, Collet TA, McKay DB, Craig SB. Leptospirosis from water sources. Pathog Glob Health. 2014; 108(7): 334–338.
30.
Doudier B, Garcia S, Quennee V, Jarno P, Brouqui P. Prognostic factors associated with severe leptospirosis. Clin Microbiol Infect. 2006; 12(4): 299–300.
31.
Wójcik-Fatla A, Zając V, Cisak E, Sroka J, Sawczyn A, Dutkiewicz J. Leptospirosis as a tick-borne disease? Detection of Leptospira spp. in Ixodes ricinus ticks in eastern Poland. Ann Agric Environ Med. 2012; 19(4): 656–659.
32.
Bertelloni F, Turchi B, Vattiata E, Viola P, Pardini S, Cerri D, et al. Serological survey on Leptospira infection in slaughtered swine in North-Central Italy. Epidemiol Infect. 2018; 146(10): 1275–1280.
33.
Strutzberg-Minder K, Tschentscher A, Beyerbach M, Homuth M, Kreienbrock L. Passive surveillance of Leptospira infection in swine in Germany. Porcine Health Manag. 2018; 4: 10.
34.
Cruz-Romero A, Alvarado-Esquivel C, Romero-Salas D, Alvarado-Félix ÁO, Sánchez-Montes S, Hernández-Tinoco J, et al. Seroepidemiology of Leptospira Infection in Backyard Pigs in Durango State, Mexico. Eur J Microbiol Immunol. 2018; 8(3): 87–90.
35.
Tagliabue S, Figarolli BM, D’Incau M, Foschi G, Gennero MS, Giordani R, et al. Serological surveillance of Leptospirosis in Italy: two year national data (2010 2011). Vet Ital. 2016; 52(2): 129–138.
36.
Ayral FC, Bicout DJ, Pereira H, Artois M, Kodjo A. Distribution of Leptospira serogroups in cattle herds and dogs in France. Am J Trop Med Hyg. 2014; 91(4): 756–759.
37.
McLean M, Ruscoe Q, Kline T, King C, Nesdale A. A cluster of three ases of leptospirosis in dairy farm workers in New Zealand. N Z Med J. 2014; 127(1388): 13–20.
38.
Dhaka P, Malik SS, Yadav JP, Kumar M, Baranwal A, Barbuddhe SB, et al. Seroprevalence and molecular detection of coxiellosis among cattle and their human contacts in an organized dairy farm. J Infect Public Health. 2019; 12(2): 190–194.
39.
Eraso-Cadena MP, Molina-Guzmán LP, Cardona X, Cardona-Arias JA, Ríos-Osorio LA, Gutierrez-Builes LA. Serological evidence of exposure to some zoonotic microorganisms in cattle and humans with occupational exposure to livestock in Antioquia, Colombia. Cad Saude Publica. 2018; 34(10): e00193617.
40.
Sroka J, Karamon J, Dutkiewicz J, Wojcik-Fatla A, Zając V, Cencek T. Prevalence of Toxoplasma gondii infection in cats in southwestern Poland. Ann Agric Environ Med. 2018; 25(3): 576–580.
41.
Esteves F, Aguiar D, Rosado J, Costa ML, de Sousa B, Antunes F, et al. Toxoplasma gondii prevalence in cats from Lisbon and in pigs from centre and south of Portugal. Vet Parasitol. 2014; 200(1–2): 8–12.
42.
Nabi H, Rashid MI, Islam S, Bajwa AA, Gul R, Shehzad W, et al. Prevalence of Toxoplasma gondii oocysts through Copro-PCR in cats at Pet Center (UVAS), Lahore, Pakistan. J Pak Med Assoc. 2018; 68(1): 115–118.
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