BRIEF COMMUNICATION
ISOLATION OF BORRELIA AFZELII FROM OVERWINTERING CULEX PIPIENS BIOTYPE MOLESTUS MOSQUITOES
 
More details
Hide details
1
Department of Comparative Animal Physiology and General Zoology, Faculty of Science, Masaryk University, Brno, Czech Republic
2
Institute of Vertebrate Biology, Department of Medicine Zoology, Academy of Sciences, Valtice, Czech Republic
3
Cenex CZ, Brno, Czech Republic
CORRESPONDING AUTHOR
Alena Žákovská   

Department of Comparative Animal Physiology & General Zoology, Faculty of Science, Masaryk University, Kotlářská 2, CZ-611 37 Brno, Czech Republic.
 
Ann Agric Environ Med. 2006;13(2):345–348
KEYWORDS
ABSTRACT
A total of 662 samples (winter period: 469; summer period: 193 specimens) of female mosquitoes of the genus Culex, Aedes and Anopheles were collected during the period March 2000-April 2001 from the locality of Vysoké Mýto (Eastern Bohemia, Czech Republic). They were examined by dark field microscopy for the presence of spirochetes. The motile spirochetes were observed in 4.2% of all species of investigated mosquitoes. One spirochetal strain out of the 8 isolation attempts (BRZ14) was obtained (cultivation rate was 12.5%) and the spirochetal strain was then successfully cultivated and identified using PCR for the presence of Borrelia burgdorferi s.l., and subsequently with the RFLP as genomospecies Borrelia afzelii. This strain was derived from overwintering Culex (Culex) pipiens biotype molestus female mosquitoe. This is apparently one of the sporadic cases of the occurrence of pathogenic borreliae in haematophagous arthropods, other than Ixodes ricinus complex ticks.
eISSN:1898-2263
ISSN:1232-1966