RESEARCH PAPER
Evaluation of anticancer activity of water and juice extracts of young Hordeum vulgare in human cancer cell lines HT-29 and A549
 
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1
Departament of Virology and Immunology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University
2
Department of Medical Biology, Institute of Rural Health, Lublin, Poland
 
Ann Agric Environ Med. 2017;24(2):345–349
KEYWORDS:
ABSTRACT:
Introduction and objective:
Barley (Hordeum vulgare L.) is known as a rich source of different bioactive compounds. At present, considerable attention of researchers is focused on young barley grass. It can be a good source of dietary minerals, vitamins, carbohydrates, amino acids, phenolic compounds and proteins. It is possible that the composition of chemical ingredients beneficial for health may induce an anticancer potential of young barley in human colon adenocarcinoma (HT-29) and human lung adenocarcinoma (A549) cell lines.

Material and Methods:
Hordeum vulgare water extract (HWE) and Hordeum vulgare juice extract (HJE) were prepared. Cell proliferation and viability were examined with the use of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and NR (neutral red) methods. Induction of necrosis was assessed by propidium iodide/Hoechst staining. Progress of the cell cycle involved in cell proliferation, apoptosis, and regulation of transcription was estimated using flow cytometry analysis. Additionally, the capability of free radical scavenging was evaluated with the DPPH assay.

Results:
The study revealed that extracts inhibited the proliferation of cancer cells. The NR study confirmed the low cytotoxic activity of the tested extracts to normal human colon epithelial cells (CCD 841 CoTr) and human skin fibroblasts (HSF). Furthermore, a dose-dependent cytotoxicity against HT-29 cells, but not A549 cells, has been reported. The free radical scavenging activity was observed in the case of the HWE but not the HJE.

Conclusions:
The obtained results indicate a cancer chemopreventive potential of young barley as a safe dietary agent in colon carcinoma.

CORRESPONDING AUTHOR:
Arkadiusz Czerwonka   
Departament of Virology and Immunology, Institute of Biology and Biochemistry, Maria Curie-Skłodowska University
 
REFERENCES (30):
1. Baik BK, Ullrich SE. Barley for food: Characteristics, improvement, and renewed interest. J Cereal Sci. 2008; 48(2): 233–242.
2. Prasad R, Prasad LC, Bornare SS. Barley (Hordeum vulgare L.) crops (Model species), its use as food, feed, medicines and performed better when climatic conditions are unfavorable. Indian Journal of Crop Ecology 2014; 2(2): 47–52.
3. Hagiwara Y, Cichoke AJ. Barley leaves extract for everlasting health. Green Foods Corp. and Japan Pharmaceutical Development. 1998: 7(10): 15.
4. Paulickova I, Ehrenbergerová J, Fiedlerova V, Gabrovská D, Havlová P, Holasová M, et al. Evaluation of barley grass as a potential source of some nutritional substances. Czech J Food Sci. 2007; 25(2): 65–72.
5. Dudjak JJ, Lachman D, Miholová D, Kolihová V, Pivec. Effect of cadmium on polyphenol content in young barley plants (Hordeum vulgare L.). Plant Soil Environ. 2004; 11(11): 471–477.
6. Meng TX, Irino N, Kondo R. Melanin biosynthesis inhibitory activity of a compound isolated from young green barley (Hordeum vulgare L.) in B16 melanoma cells. J Nat Med. 2015; 69(3): 427–431.
7. Jeong HJ, Lam Y, Lumen BO. Barley Lunasin supresses ras-induced colony formation and inhibits core histone acetylation in mammalian cells. J Agric Food Chem. 2002; 50: 5903–5908.
8. Izydorczyk MS, Dexter JE. Barley β-glucans and arabinoxylans: Molecular structure, physicochemical properties, and uses in food products–a Review. Food Res Int. 2008; 41(9): 850–868.
9. Pins JJ, Kaur H. A review of the effects of barley β-glucan on cardiovascular and diabetic risk. Cereal Foods World. 2006; 51(1): 8–11.
10. Kim H, Kwak BS, Hong HD, Suh HJ, Shin KS. Structural features of immunostimulatory polysaccharide purified from pectinase hydrolysate of barley leaf. Int J Biol Macromol. 2016; 87: 308–316.
11. Oliveira RJ, Ribeiro LR, da Silva AF, Matuo R, Mantovani MS. Evaluation of antimutagenic activity and mechanisms of action of beta-glucan from barley, in CHO-kl and HTC cell lines usin micronucleus test. Toxicol In Vitro. 2006; 20: 1225–1233.
12. Robles-Escajeda E, Lerma D, Nyakeriga AM, Ross JA, Kirken RA, Aguilera RJ, et al. Searching in mother nature for anti-cancer activity: antiproliferate and pro-apoptic effect elicited by green barley on leukemia/lymphoma cells. PLoS ONE. 2013; 8(9): 1–16.
13. Yamaura K, Nakayama N, Shimada M, Bi Y, Fukata H, Ueno K. Antidepressant-like effects of young green barley leaf (Hordeum vulgare L.) in the mouse forced swimming test. Pharmacognosy Res. 2012; 4: 22−26.
14. Kanauchi O, Mitsuyama K, Andoh A, Iwanaga T. Modulation of intestinal environment by prebiotic germinated barley foodstuff prevents chemo-induced colonic carcinogenesis in rats. Oncol Rep. 2008; 20: 793–801.
15. Ohtake H, Yuasa H, Komura C, Miyauchi T, Hagiwara Y, Kubota K. Studies on the constituents of green juice from young barley leaves. Antiulcer activity of fraction from barley juice. Yakugaku Zasshi. 1985; 105: 1045–1051.
16. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin. 2011; 61(2): 69–90.
17. Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015; 136(5): 359–386.
18. Siegel R, Naishadham D, Jemal A. Cancer statistics, 2012. CA Cancer J Clin. 2012; 62(1): 10–29.
19. Mihajlovic-Bozic V. Risk factors for colorectal cancer. Arch Oncol. 2004; 12(1): 45–49.
20. Haggar FA, Boushey R.P. Colorectal cancer epidemiology: Incidence, mortality, survival, and risk factors. Clin Colon Rectal Surg. 2009; 22(4): 191–197.
21. Brevik A, Joshi AD, Corral R, Onland-Moret NC, Siegmund KD, Le Marchand L, et al. Polymorphisms in base excision repair genes as colorectal cancer risk factors and modifiers of the effect of diets high in red meat. Cancer Epidemiol Biomarkers Prev. 2010; 19(12): 3167–3173.
22. Matysiak J, Juszczak M, Karpińska MM, Langner E, Walczak K, Lemieszek MK, et al. Synthesis of 2-(2,4-dihydroxyphenyl)thieno-1,3-thiazin-4-ones, their lipophilicity and anticancer activity in vitro. Mol Divers. 2015; 19(4): 725–36.
23. Paduch R, Trytek M, Król SK, Kud J, Frant M, Kandefer-Szerszeń M, et al. Biological activity of terpene compounds produced by biotechnological methods. J Pharmaceutical Biology 2016; 54(6): 1096–1107.
24. Jakubowicz-Gil J, Langner E, Bądziul D, Wertel I, Rzeski W. Quercetin and sorafenib as a novel and effective couple in programmed cell death induction in human gliomas. Neurotox Res. 2014. 26(1): 64–77.
25. Sławińska-Brych A, Król SK, Dmoszyńska-Graniczka M, Zdzisińska B, Stepulak A, Gagoś M. Xanthohumol inhibits cell cycle progression and proliferation of larynx cancer cells in vitro. Chem. Biol. Interact. 2015; 240: 110–118.
26. Litchfield JT, Wilcoxon FA. A simplified method of evaluating dose-effect experiments. J Pharmacol Exp Ther. 1949; 96: 99–113.
27. Kubatka P, Kello M, Kajo K, Kruzliak P, Výbohová D, Šmejkal K, et al. Young barley indicates antitumor effects in experimental breast cancer in vivo and in vitro. Nutr Cancer. 2016; 68(4): 611–621.
28. Ikeguchi M, Tsubata M, Takano A, Kamiya T, Takagaki K, Ito H, et al. Effects of young barley leaf powder on gastrointestinal functions in rats and its efficacy-related physicochemical properties. Evid Based Complement Alternat Med. 2014; 2014: 7.
29. Sato A. Studies on anti-tumor activity of crude drugs. 1. The effects of aqueous extracts of some crude drugs in short-term screening test. Yakugaku Zasshi. 1989; 109(6): 407–423.
30. Kamiyama M, Shibamoto T. Flavonoids with potent antioxidant activity found in young green barley leaves. J Agric Food Chem. 2012; 60(25): 6260–6267.
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