RESEARCH PAPER
Evaluation of CD40 and CD80 receptors in the colonic mucosal membrane of children with inflammatory bowel disease
 
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1
Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, Poland
2
Department of Developmental Age Medicine and Paediatric Nursing, Medical University of Bialystok, Poland
3
Department of Pathomorphology, Medical University of Bialystok, Poland
CORRESPONDING AUTHOR
Barbara Kamińska   

Department of Pediatrics, Pediatric Gastroenterology, Hepatology and Nutrition, Medical University of Gdansk, Poland
 
Ann Agric Environ Med. 2015;22(4):695–699
KEYWORDS
ABSTRACT
Introduction:
The most prevalent inflammatory bowel diseases (IBD) include ulcerative colitis (UC) and Crohn’s disease (CD). Immune processes play a vital role in the etiopathogenesis of these conditions, involving both cellular and humoral response mechanisms. The aim of this study was to quantify CD40- and CD80-positive cells in the biopsy specimens of large intestinal mucosa from children with IBD.

Material and Methods:
The study comprised 38 children aged between 3–17 years (mean 11.5±3.7 years) – 20 boys (52.6 %) and 18 girls (47.4%). Eighteen patients were diagnosed with UC on the basis of clinical manifestation, endoscopic and histopathological findings. Mean age of this subgroup was 11.55±4.07 years. A group of 10 children (mean age 12.30±2.83) diagnosed with CD was also included. The control group comprised 10 IBD-free children (mean age 10.28±4.07 years). The surface expressions of CD40 and CD80 were analyzed in large intestine mucosa biopsy specimens, fixed in formaldehyde, embedded in paraffin, and cut with a microtome into 4 µm slices.

Results:
The number of CD40- and CD80-positive cells in the large intestinal mucosa of children with Crohn’s disease and ulcerative colitis was significantly higher than in the controls. The highest number of CD40+ and CD80+ cells was observed in the caecal mucosal membrane of Crohn’s disease patients and in the rectal mucosa of individuals with ulcerative colitis.

Conclusions:
IBD is characterized by elevated, segment-specific, expression of CD40 and CD80.

 
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