Antibiotic resistance of [i]Escherichia coli[/i] isolated from a poultry slaughterhouse
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University of Veterinary Medicine and Pharmacy, Kosice, Slovakia
Faculty of Medicine, Department of Medical and Clinical Microbiology, P. J. Safarik University, Kosice, Slovakia
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia
Slovak University of Agriculture, Nitra, Slovakia
Ann Agric Environ Med 2012;19(1):75–77
The aim of the study was to investigate the antibiotic resistant [i]E. coli[/i] strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43 % isolates. The higher MIC of ceftazidime (3.6 mg.l[sup]-1[/sup]) and ceftriaxon (5.2 mg.l[i]-1[/i]) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected [i]E. coli[/i] showed a clear clonal relatedness of environmental strains from various withdrawals.