Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse
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University of Veterinary Medicine and Pharmacy, Kosice, Slovakia
Faculty of Medicine, Department of Medical and Clinical Microbiology, P. J. Safarik University, Kosice, Slovakia
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia
Slovak University of Agriculture, Nitra, Slovakia
Ann Agric Environ Med 2012;19(1):75–77
The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43 % isolates. The higher MIC of ceftazidime (3.6 mg.l-1) and ceftriaxon (5.2 mg.l-1) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.
Vladimir Kmet   
Institute of Animal Physiology, Slovak Academy of Sciences, Kosice, Slovakia
1. Tsola E, Drosinos EH, Zoiopoulos P. Impact of poultry slaughter house modernisation and updating of food safety management systems on the microbiological quality and safety of products. Food Control. 2008; 19: 423-431.
2. Dieckmann R, Malorny B. Rapid screening of epidemiologically important Salmonella enterica subsp. enterica serovars by whole-cell matrix-assisted laser desorption ionization–time of flight mass spectrometry. Appl Environ Microbiol. 2011; 77: 4136-4146.
3. Sauer S, Freiwald A, Maier T, Kube M, Reinhardt R, Kostrzewa M, Geider K. Classification and identification of bacteria by mass spectrometry and computational analysis. PLoS One. 2008; 3(e2843): 1-10.
4. Clinical Laboratory Standards Institute. Performance Standards for Antimicrobial Disk and Dilution. Susceptibility Tests for Bacteria Isolated from Animals (3rd edn.). CLSI Document M31-A3 28, 2008: 1-99.
5. Gattringer R, Niks M, Ostertag R, Schwarz K, Medvedovic H, Graninger W, Georgopoulos A. Evaluation of Miditech automated colorimetric MIC reading for antimicrobial susceptibility testing. J Antimicrob Chemother. 2002; 49: 651-659.
6. Livermore DM, Winstanley TG, Shannon KP. Intepretative reading: recognising unusual and interferring resistance mechanisms from resistance phenotypes. J Antimicrob Chemother. 2001; 48(Suppl. S1): 87-102.
7. Carattoli A, Garcia-Fernandez A, Varesi P, Fortini D, Gerardi S, Penni A, Mancini C, Giordano A. Molecular epidemiology of Escherichia coli producing extended-spectrum ß-lactamases isolated in Rome, Italy. J Clin Microbiol. 2008; 46: 103-108.
8. Pérez-Pérez PJ, Hanson ND. Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR. J Clin Microbiol. 2002; 40: 2153-2162.
9. Bolder MN. The microbiology of the slaughter and processing of poultry. In: Davies A, Board R (Ed): The Microbiology of Meat and Poultry, 158-173, Springer – Verlag, Heidelberg, 1998.
10. Fernandez-Lopez J, Sendra-Nadal E, Sayas-Barbera E. Slaughtering equipment and operations. In: Guerrero-Legarreta I (Ed.): Handbook of Poultry Science and Technology, Primary Processing. John Wiley & Sons, Inc., New Jersey 2009: 79-100.
11. Marriott NG. Essentials of Food Sanitation, Sources of Food Contamination. Chapman and Hall, New York 1997.
12. Brtkova A, Bujdakova H. Antibiotic resistance in Enterococcus isolates from poultry swabs in Slovakia. J Food Nutr Res. 2009; 48: 121-128.
13. Drugdova Z, Kmet V, Bujnakova D. Virulence factors in Escherichia coli isolated from chicken meat in Slovakia. J Food Nutr Res. 2010; 49: 10-13.
14. Kmet V, Bujnakova D, Drugdova Z, Kmetová M. Betalactam resistance in food Escherichia coli isolated from broilers. In: Mendez-Vilas A (Ed.): Microorganisms in Industry and Environment. From Scientific and Industrial Research to Consumer Products, World Scientific Pub Co. Pte Ltd, Singapore 2011: 363-365.
15. Warren RE, Ensor VM, O’Neill P, Butler V, Taylor J, Nye K, Harvey M, Livermore DM, Woodford N, Hawkey PM. Imported chicken meat as a potential source of quinolone-resistant Escherichia coli producing extended-spectrum ß-lactamases in the UK. J Antimicrob Chemother. 2008; 61: 504-508.