RESEARCH PAPER
Total phenolic content, FTIR analysis, and antiproliferative evaluation of lupin seeds harvest from western Romania
1
University of Medicine and Pharmacy “Victor Babeş”, Timişoara, Romania
2
Banat’s University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania”, Timisoara, Romania
3
Department of Microbiology, Discipline of Hygiene, Faculty of Pharmacy, University of Medicine and Pharmacy “Victor Babeş“, Timişoara, România
Corresponding author
Ann Agric Environ Med. 2017;24(4):726-731
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Lupinus spp. are herbaceous perennial flowering plants included in the Fabaceae family. Among the approximately 200 species belonging to this genre, Lupinus albus L., also known as white lupin, Lupinus angustifolius L., and narrow-leafed lupin or blue lupin, represent two of the most studied species due to their intense culinary use and potential biological activity. The intention of the study was to characterize total phenolic content, perform FTIR analysis, and antiproliferative effects against A375 human melanoma cells extracts obtained from germinated and ungerminated seeds from Lupinus albus L. and Lupinus angustifolius L.
Material and methods:
Total phenolic content was assessed using the Folin-Ciocalteu colorimetric method. FTIR spectra were carried out by a Shimadzu Prestige-21 spectrometer in the range 400–4000 cm-1, using KBr pellets and resolution of 4 cm-1. Antiproliferative capacity was screened by employing the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and scratch assay methods.
Results:
The study showed increased values corresponding to total phenolic content for the germinated extracts. FTIR spectra confirmed the presence of genistein and main cinnamic acids derivatives (ferulic, caffeic, rosmarinic, and coumaric acids). All tested extracts showed weak antiproliferative potential against A375 human melanoma cells.
Conclusions:
Germination increased the amount of bioactive compounds in the seeds of the two studied species of lupin. FTIR analyses provided an important fingerprint of the chemical composition.
Lupinus spp. are herbaceous perennial flowering plants included in the Fabaceae family. Among the approximately 200 species belonging to this genre, Lupinus albus L., also known as white lupin, Lupinus angustifolius L., and narrow-leafed lupin or blue lupin, represent two of the most studied species due to their intense culinary use and potential biological activity. The intention of the study was to characterize total phenolic content, perform FTIR analysis, and antiproliferative effects against A375 human melanoma cells extracts obtained from germinated and ungerminated seeds from Lupinus albus L. and Lupinus angustifolius L.
Material and methods:
Total phenolic content was assessed using the Folin-Ciocalteu colorimetric method. FTIR spectra were carried out by a Shimadzu Prestige-21 spectrometer in the range 400–4000 cm-1, using KBr pellets and resolution of 4 cm-1. Antiproliferative capacity was screened by employing the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) and scratch assay methods.
Results:
The study showed increased values corresponding to total phenolic content for the germinated extracts. FTIR spectra confirmed the presence of genistein and main cinnamic acids derivatives (ferulic, caffeic, rosmarinic, and coumaric acids). All tested extracts showed weak antiproliferative potential against A375 human melanoma cells.
Conclusions:
Germination increased the amount of bioactive compounds in the seeds of the two studied species of lupin. FTIR analyses provided an important fingerprint of the chemical composition.
REFERENCES (28)
1.
Cragg GM, Newman DJ Natural products: a continuing source of novel drug leads. Biochimica et Biophysica Acta. 2013; 1830(6): 3670–3695.
2.
Dias DA, Urban S, Roessner U. A Historical Overview of Natural Products in Drug Discovery. Metabolites. 2012; 2(2): 303–336.
3.
Gulewicz P, Martinez-Villaluenga C, Kasprowicz-Potocka M, Frias J. Non-Nutritive Compounds in Fabaceae Family Seeds and the Improvement of Their Nutritional Quality by Traditional Processing – a Review. Pol J Food Nutrition Sci. 2014; 64(2): 75–89.
4.
Andor B, Danciu C, Alexa E, Zupko I, Hogea E, Cioca A, et al. Germinated and Ungerminated Seeds Extract from Two Lupinus Species: Biological Compounds Characterization and In Vitro and In Vivo Evaluations. Evidence-Based Complementary and Alternative Medicine.2016;2016(7638542):1–8. http://dx.doi.org/10.1155/2016....
5.
Monteiro MRP, Costa ABP, Campos SF, Silva MR, da Silva CO, Martino HSD, Silvestre MPC. Evaluation of the chemical composition, protein quality and digestibility of lupin (Lupinus albus and Lupinus angustifolius), O mundo da Saude. 2014; 38(3): 251–259.
6.
Erdemoglu N, Ozkan S, Tosun F. Alkaloid profile and antimicrobial activity of Lupinus angustifolius L. alkaloid extract. Phytochemistry Rev. 2007; 6(1): 197–201.
7.
Tsaliki E, Lagouri V, Doxastakis G. Evaluation of the antioxidant activity of lupin seed flour and derivatives (Lupinus albus ssp. Graecus). Food Chemistry. 1999; 65(1): 71–75.
8.
Lampart-Szczapa E, Siger A, Trojanowska K, Nogala-Kalucka M, Malecka M, Pacholek B. Chemical composition and antibacterial activities of lupin seeds extracts. Nahrung. 2003; 47(5): 286–290.
9.
Sirtori CR, Lovati MR, Manzoni C, Castiglioni S, Duranti M, Magni C, et al. Proteins of White Lupin Seed, a Naturally Isoflavone-Poor Legume, Reduce Cholesterolemia in Rats and Increase LDL Receptor Activity in HepG2 Cells. J Nutr. 2004; 134(1): 18–23.
10.
Martins JM, Riottot M, Abreu MC de, Viegas-Crespo AM, Lança MJ, Almeida JA, et al. Cholesterol-lowering effects of dietary blue lupin (Lupinus angustifolius L.) in intact and ileorectal anastomosed pigs. J Lipid Res. 2005; 46(7): 1539–1547.
11.
Marchesi M, Parolini C, Diani E, Rigamonti E, Cornelli L, Arnoldi A, et al. Hypolipidaemic and anti-atherosclerotic effects of lupin proteins in a rabbit model. Br J Nutr. 2008; 100(4): 707–710.
12.
Lovati MR, Manzoni C, Castiglioni S, Parolari A, Magni C, Duranti M. Lupin seed γ-conglutin lowers blood glucose in hyperglycaemic rats and increases glucose consumption of HepG2 cells. Br J Nutr. 2012; 107(1): 67–73.
13.
Dubois O, Sallé G, Février H, Magnin-Robert JB, Harzic N, Charvet C, et al. Characterization of anthelminthic properties of the lupine seed, Lupinus spp. Planta Medica. 2016; 82(S 01): P1006.
14.
Pandey KB, Rizvi SI. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longev. 2009; 2(5): 270–278.
15.
Habauzit V, Morand C. Evidence for a protective effect of polyphenols-containing foods on cardiovascular health: an update for clinicians. Ther Adv Chronic Dis. 2012; 3(2): 87–106.
16.
Dai J, Mumper RJ. Plant phenolics: extraction, analysis and their antioxidant and anticancer properties. Molecules. 2010;15(10):7313–7352.
17.
Wang S, Clements J. Antioxidant activities of lupin seeds. In: Lupins for Health and Wealth. Proceedings of the 12th International Lupin Conference, Fremantle, Western Australia. 2008; 14–18: 546–551.
18.
Siger A, Czubinski J, Kachlicki P, Dwiecki K, Lampart-Szczapa E, Nogala-Kalucka M. Antioxidant activity and phenolic content in three lupin species. J Food Comps Anal. 2012; 25(2): 190–197.
19.
Lin PY, Lai HM. Bioactive compounds in legumes and their germinated products. J Agr Food Chem. 2006; 54(11): 3807–3814.
20.
Duenas M, Hernandez T, Estrella I, Fernandez D. Germination as a process to increase the polyphenol content and antioxidant activity of lupin seeds (Lupinus angustifolius L.). Food Chem. 2009; 117(4): 599–607.
21.
Coates J. Interpretation of Infrared Spectra, A Practical Approach. In Encyclopedia of Analytical Chemistry, Meyers R.A. (Ed.); John Wiley & Sons, Ltd: Chichester, UK, 2000; 10815–37.
22.
Segneanu AE, Gozescu I, Dabici A, Sfirloaga P, Szabadai, Z. Organic Compounds FT-IR Spectroscopy, Macro To Nano Spectroscopy, Uddin J. (Ed.), InTech, 2012.
23.
Stuart BH Infrared Spectroscopy: Fundamentals and Applications. Methods. Analytical Techniques in the Sciences, Ando DJ (Ed.), England, Wiley, 2004; 8.
24.
Nolasco M, Amado AM, Ribeiro-Claro PJA. Effect of hydrogen bonding in the vibrational spectra of trans-cinnamic acid. J Raman Spectrosc. 2009; 40(4): 394–400.
25.
Crupi V, Ficarra R., Guardo M, Majolino D, Stancanelli R, VenutiV UV-vis and FTIR-ATR spectroscopic techniques to study the inclusion complexes of genistein with β-cyclodextrins. J Pharmaceut Biomed Analysis. 2007; 44(1): 110–117.
26.
Silverstein RM, Webster F., Kiemle DJ. Spectrometric Identification of Organic Compounds. 7th ed.; John Wiley and Sons: Hoboken, NJ, 2005.
27.
Timeus F, Crescenzio N, Fandi A, Doria A, Foglia L, Cordero di Montezemolo L. In vitro antiproliferative and antimigratory activity of dasatinib in neuroblastoma and Ewing sarcoma cell lines. Oncol Reports. 2008; 19(2): 353–359.
28.
Bau HM, Villaume C, Méjean L. Effects of soybean (Glycine max) germination on biologically active components, nutritional values of seeds, and biological characteristics in rats. Nahrung. 2000; 44(1): 2–6.
| eISSN: | 1898-2263 |
| ISSN: | 1232-1966 |
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
© 2006-2024 Journal hosting platform by Bentus
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
