Serological and molecular evidence of Coxiella burnetii in samples from humans and animals in China
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Jiangsu Co-innovation Centre for the Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou University College of Veterinary Medicine, Jiangsu, China
Department of Animal Hygiene, Zoonoses and Animal Behavior and Management, Faculty of Veterinary Medicine, Suez Canal University, Egypt
Ross University School of Veterinary Medicine, West Indies
Laboratory of Zoonosis, China Animal Health and Epidemiology Centre, Shandong, China
Yangzhou University College of Medicine, Yangzhou 225009, Jiangsu, China
Ann Agric Environ Med. 2016;23(1):87-91
Coxiella burnetii is the agent of Q fever, a worldwide zoonosis. To add to the available knowledge of the disease in China, C. burnetti infections were investigated in convenience samples from five animal species and humans from Yangzhou, Jiangsu province, eastern China.

Material and Methods:
Commercial ELISA kits were used to detect antibodies to phase I and II C. burnetii. A FRET-qPCR targeting the outer membrane protein com1 gene was also developed to detect C. burnetii DNA in blood samples from animals and humans, and bovine milk samples.

Seropositive cattle (44/150; 29%), goats (33/150; 22%), humans (45/180; 25%) and pigs (4/130; 3%) were found, while dogs (0/136; 0%) and cats (0/140; 0%) were seronegative. Seropositivity in humans was associated with increasing age, but there was no gender difference. DNA was amplified from two milk samples (2/150, 1.3%), while none of the blood samples were positive. The sequences of the obtained amplicons were identical to those of the com1 gene of the universal C. burnetii RSA 493 strain and other stains from China.

The findings indicaten that C. burnetii is endemic in Yangzhou, China, and therefore human and animal health workers should be aware of the possibility of infections and the occurrence of outbreaks of Q fever.

Arricau-Bouvery N, Rodolakis A. Is Q fever an emerging or re-emerging zoonosis? Vet Res. 2005; 36: 327–349.
Maurin M, Raoult D. Q fever. Clin Microbiol Rev. 1999; 12: 518–553.
Hatchette TF, Hudson RC, Schlech WF, Campbell NA, Hatchette JE, Ratnam S, et al. Goat-associated Q fever: a new disease in Newfoundland. Emerg Infect Dis. 2001; 7(3): 413–419.
Kazar J. Coxiella burnetii infection. Ann N Y Acad Sci. 2005; 1063: 105–114.
Porter SR, Czaplicki G, Mainil J, Guatteo R, Saegerman C. Q Fever: current state of knowledge and perspectives of research of a neglected zoonosis. Int J Microbiol. 2011; 248418.
Bielawska-Drózd A, Cieślik P, Mirski T, Gaweł J, Michalski A2, Niemcewicz M, Bartoszcze M, Żakowska D, Lasocki K, Knap J, Kocik J. Prevalence of Coxiella burnetii in environmental samples collected from cattle farms in Eastern and Central Poland (2011–2012). Vet Microbiol. 2014; 174(3–4): 600–606.
Schimmer B, Dijkstra F, Velllema P, Schneeberger PM, Hackert V, ter Schegget R, et al. Sustained intensive transmission of Q fever in the south of the Netherlands. Euro Surveill. 2009; 14(19). pii:19210.
Marrie TJ, Langille D, Papukna V, Yates L. Truckin’ pneumonia: an outbreak of Q fever in a truck repair plant probably due to aerosols from clothing contaminated by contact with newborn kittens. Epidemiol Infect. 1989; 102(1): 119–127.
Roest HI, Tilburg JJ, van der Hoek W, Vellema P, van Zijderveld FG, Klaassen CH, et al. The Q fever epidemic in The Netherlands: history, onset, response and reflection. Epidemiol Infect. 2011; 139: 1–12.
Tissot-Dupont H, Raoult D. Q fever. Infect Dis Clin North Am. 2008; 22(3): 505–514.
Angelakis E, Raoult D. Q fever. Vet Microbiol. 2010; 140: 297–309.
Zhang NC, Xie SW, Liu FT, Zhao SX. Preliminary study on the problem of Q fever in Beijing. Chin Med J. 1951; 37: 235.
Yu S. Chapter 12: Coxiella burnetii in China. In: Williams JC and Thompson HA, editors. Q Fever: The Biology of Coxiella burnetii. CRC Press: 1991. p. 327–339.
El-Mahallawy HS, Lu G, Kelly P, Xu D, Li Y, Fan W et al. Q fever in China: a systematic review, 1989–2013. Epidemiol Infect. 2015; 143: 673–681.
Zhang J, Wei L, Kelly P, Freeman M, Jaegerson K, Gong J, et al. Detection of Salmonella spp. using a generic and differential FRET-PCR. PLoS One. 2013; 8(10): e76053.
Tan Z, Li L, Zhang L. Cross-sectional survey on the prevalence of antibodies to several types of Rickettsia in human and livestock in Jiangsu province. J Soochow Univ Med Sci Edit. 2012; 32: 445–550.
Wang SQ, Yang YZ, Sun CZ, Wang ZH, Zhu LY, He SQ, et al. Study on the infection of Q fever in Huaibei. Chin J Zoon. 1993; 9: 19–20.
Li Z, Chen T, Huang JT. Preliminary investigation on the seroepidemiology of Q fever among animals in Shandong Province. Chin J Zoonoses. 1996; 12(1): 12–3.
Zhang D, Lu J. Sero-epidemiologic investigation on rickettsiosis of humans and livestock in Lushi County. Henan J Preven Med. 2013; 24: 418–421.
Chang L, Dao Z, Liang C, Li J, Li Y, Zhao J, et al. Sero-epidemiologic investigation on rickettsiosis of humans and domestic animals in Yunnan province. Chin J Zoonoses. 2010; 2: 189–192.
Zhang Y, Shi Y, Liu H, Cao M, Hu W, Wang J, et al. Seroepidemiological Investigation on Q fever of people and livestock in different regions of Anhui province. Anhui J Prev Med. 2010; 16(2): 87–89.
Sun Y, Dou XF, Lu YN, Wang XM, Guan ZZ, Wang QY. Serological investigation on Q fever in stockbreeding-related population in Beijing area. Occup Health. 2013; 29: 2967–2971.
Karabay O, Kocoğlu E, Baysoy G, Konyalioğlu S. Coxiella burnetii seroprevalence in the rural part of Bolu, Turkey. Turk J Med Sci. 2009; 39(4): 641–645.
Vilibic-Cavlek T, Kucinar J, Ljubin-Sternak S, Kolaric B, Kaic B, Lazaric-Stefanovic L, et al. Prevalence of Coxiella burnetii antibodies among febrile patients in Croatia, 2008–2010. Vector Borne Zoonotic Dis. 2012; 12: 293–296.
Stein A, Raoult D. Detection of Coxiella burnetii by DNA Amplification using Polymerase Chain Reaction. J Clin Microbiol. 1992; 30(9): 2462–2466.
Fournier PE, Raoult D. Comparison of PCR and serology assays for early diagnosis of acute Q fever. J Clin Microbiol. 2003; 41(11): 5094–5098.
Kelly PJ. Q fever. In: JAW Coetzer, GR Thompson, RC Tustin, editors. Infectious Diseases of Livestock with Special Reference to Southern Africa. 2nd Ed. Eds. Oxford University Press. 2005. Volume 1. p. 565–72.
Rodolakis A. Q fever in Dairy Animals. Rickettsiology and Rickettsial Diseases-Fifth International Conference: Ann NY Acad Sci. 2009; 1166: 90–93.
Pan L, Zhang L, Fan D, Zhang X, Liu H, Lu Q, et al. Rapid simple and sensitive detection of Q fever by loop-mediated isothermal amplification of the htpAB gene. PLoS Neg Trop Dis. 2013; 7(5): e2231.
Kim SG, Kim EH, Lafferty CJ, Dubovi E. Coxiella burnetii in bulk tank milk samples, United States. Emerg Infect Dis. 2005; 11(4): 619–621.
Muskens J, van Engelen E, van Maanen C, Bartels C, Lam TJ. Prevalence of Coxiella burnetii infection in Dutch dairy herds based on testing bulk tank milk and individual samples by PCR and ELISA. Vet Rec. 2011; 168(3): 79.
Pimenta L, Alegria N, Anastácio S, Sidi-Boumedine K, da Silva G, Rabiço Â, et al. Prevalence of Coxiella burnetii antibodies in Portuguese dairy cattle herds. Trop Anim Health Prod. 2015; 47(1): 227–230.
Gale P, Kelly L, Mearns R, Duggan J, Snary EL. Q fever through consumption of unpasteurised milk and milk products-a risk profile and exposure assessment. J Appl Microbiol. 2015; 118: 1083–95.
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