RESEARCH PAPER
Prevalence and antimicrobial resistance of Salmonella in meat and meat products in Latvia
Aivars Bērziņš
1,2
1
Institute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Agriculture, K. Helmaņa iela 8, LV-3004, Jelgava, Latvia
2
Institute of Food Safety, Animal Health and Environment ‘BIOR’, Lejupes iela 3, LV-1067, Rīga, Latvia
Corresponding author
Margarita Terentjeva
Institute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Agriculture, K. Helmaņa iela 8, LV-3004, Jelgava, Latvia
Institute of Food and Environmental Hygiene, Faculty of Veterinary Medicine, Latvia University of Agriculture, K. Helmaņa iela 8, LV-3004, Jelgava, Latvia
Ann Agric Environ Med. 2017;24(2):317–321
KEYWORDS
ABSTRACT
Introduction and objective:
Salmonella is a foodborne pathogen which causes gastrointestinal illness in consumers, and exhibits resistance to antimicrobials of eterinary and clinical significance. The aim of this study is to detect the prevalence and antimicrobial resistance of Salmonella isolates from meat in Latvia.
Material and Methods:
A total of 3,152 samples of raw and ready-to-eat (RTE) meats were collected during the official control and in-house control procedures in 2015. Samples were tested in accordance with ISO 6579:2002. All S. Typhimurium, S. Enteritidis and other isolates recovered from the official control samples (S. Derby, S. Give) were tested for antimicrobial resistance. The minimum inhibitory concentration (MIC) values were investigated in line with the requirements of the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
Results:
The prevalence of Salmonella was 0.8% (25/3152). The highest prevalence (1.5%) of Salmonella was found in minced meat and meat preparations (7/481), while the lowest (0%) in frozen meat and meat preparations (0/349) and RTE meats (0/364). The most common serovars were S. Typhimurium (36%, 9/25) and S. Derby (32%, 8/25). In total, 62% (13/21) of Salmonella isolates were resistant to at least one antimicrobial agent. Altogether, 40% (8/20) of isolates were resistant to sulfamethoxazole, 25% (5/20) to nalidixic acid, ciprofloxacin, ampicillin and 20% (4/20) to tetracycline. All isolates were susceptible to ceftazidime, cefotaxime, meropenem, azithromycin and tigecycline. S. Typhimurium exhibited antimicrobial resistance more often (87.5%) than other serovars.
Conclusions:
The study shows that the presence of Salmonella in meat, together with the high prevalence of resistant strains, is a significant public health related issue in Latvia.
Salmonella is a foodborne pathogen which causes gastrointestinal illness in consumers, and exhibits resistance to antimicrobials of eterinary and clinical significance. The aim of this study is to detect the prevalence and antimicrobial resistance of Salmonella isolates from meat in Latvia.
Material and Methods:
A total of 3,152 samples of raw and ready-to-eat (RTE) meats were collected during the official control and in-house control procedures in 2015. Samples were tested in accordance with ISO 6579:2002. All S. Typhimurium, S. Enteritidis and other isolates recovered from the official control samples (S. Derby, S. Give) were tested for antimicrobial resistance. The minimum inhibitory concentration (MIC) values were investigated in line with the requirements of the European Committee on Antimicrobial Susceptibility Testing (EUCAST).
Results:
The prevalence of Salmonella was 0.8% (25/3152). The highest prevalence (1.5%) of Salmonella was found in minced meat and meat preparations (7/481), while the lowest (0%) in frozen meat and meat preparations (0/349) and RTE meats (0/364). The most common serovars were S. Typhimurium (36%, 9/25) and S. Derby (32%, 8/25). In total, 62% (13/21) of Salmonella isolates were resistant to at least one antimicrobial agent. Altogether, 40% (8/20) of isolates were resistant to sulfamethoxazole, 25% (5/20) to nalidixic acid, ciprofloxacin, ampicillin and 20% (4/20) to tetracycline. All isolates were susceptible to ceftazidime, cefotaxime, meropenem, azithromycin and tigecycline. S. Typhimurium exhibited antimicrobial resistance more often (87.5%) than other serovars.
Conclusions:
The study shows that the presence of Salmonella in meat, together with the high prevalence of resistant strains, is a significant public health related issue in Latvia.
REFERENCES (29)
1.
Scallan E, Hoekstra RM, Angulo FJ., Tauxe RV, Widdowson MA, Roy SL, Jones JL, Griffin PM. Foodborne illness acquired in the United States-major pathogens. Emerg Infect Dis. 2011; 17: 7–15.
2.
EFSA and ECDC. The European Union summary report on trends and sources of zoonoses, zoonotic agents and food-borne outbreaks in 2013. EFSA Journal. 2015; 13: 3993.
3.
Popoff MY. Antigenic formulas of the Salmonellaserovars, 8 th revision. WHO Collaborating Centre for Reference and Research on Salmonella. Institute Pasteur, Paris, France. 2001.
5.
Little CL, Richardson JF, Owen RJ, de Pinna E., Threlfall EJ. Campylobacter and Salmonella in raw red meats in the United Kingdom: prevalence, characterization and antimicrobial resistance pattern, 2003–2005. Food Microbiol. 2008; 25: 538–543.
6.
Pochop J, Kačaniová M, Hleba L, Lejková J, Fikselová M, Kunová S,Kluz M. The StepOne real-time polymerase chain reaction detection of Salmonella sp., Salmonella enterica ser. typhimurium and enteritidis in milk and meat. J Environ Sci Health B. 2011; 46: 697–702.
7.
Schwaiger K, Huther S, Hölzel C, Kämpf P, Bauer J. Prevalence of antibiotic-resistant Enterobacteriaceae isolated from chicken and porkmeat purchases at the slaughterhouse and at retail in Bavaria, Germany. Int J Food Microbiol. 2012; 154: 206–211.
8.
Kramarenko T, Nurmoja I, Kärssin A, Meremäe K, Hörman A, Roasto M. The prevalence and serovar diversity of Salmonella in various food products in Estonia. Food Control. 2014; 42: 43–47.
9.
Collignon P, Powers JH, Chiller TM, Aidara-Kane A, Aarestrup FM. World health organization ranking of antimicrobials according to their importance in human medicine: a critical step for developing risk management strategies for the use of antimicrobials in food production animals. Clin Infect Dis. 2009; 49: 132–141.
10.
ISO. Microbiology of food and animal feeding stuffs. Horizontal method for detection of Salmonella spp. Geneva: International Organization for Standartization. 2002; 6579.
11.
CLSI. Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute. 2013. Supplement M100-S22.
12.
Commission Implementing Decision No 652/ 2013 of 12 November 2013 on the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria. http://eur-lex.europa.eu/LexUr....
13.
Mąka Ł, Maćkiw E, Ściezyńska H, Pawłowska K, Popowska M. Antimicrobial susceptibility of Salmonella strains isolated from retail meat products in Poland between 2008 and 2012. Food Control. 2014; 36: 199–204.
14.
Manios SG, Grivokostopoulos NC, Bikouli VC, Doultsos A, Zilelidou EA, Gialitaki MA, Skandamis PN. A 3-year hygiene and safety monitoring of a meat processing plant which uses raw materials of global origin. Int J Food Microbiol. 2015; 209: 60–69.
15.
D’Ostuni V, Tristezza M, De Giorgi MG, Rampino P, Grieco F, Perrotta C. Occurrence of Listeria monocytogenes and Salmonella spp. in meat processed products from industrial plants in Southern Italy. Food Control. 2016; 62: 104–109.
16.
Jordan E, Egan J, Dullea C, Ward J, McGillicuddy K, Murray G, Murphy A, Bradshaw B, Leonard N, Rafter P, McDowell S. Salmonella surveillance in raw and cooked meat and meat products in the Republic of Ireland from 2002 to 2004. Int J Food Microbiol. 2006; 112: 66–70.
17.
Collard JM, Bertrand S, Dierick K, Godard C, Wildemauwe C, Vermeersch K, Duculot J, Van Immerseel F, Pasmans F, Imberechts H, Quinet C. Drastic decrease of SalmonellaEnteritidis isolated from humans in Belgium in 2005, shift in phage types and influence on foodborne outbreaks. Epidemiol Infect. 2008; 136: 771–781.
18.
Pieskus J, Milius J, Michalskiene I, Zagrebneviene G. The distribution of Salmonellaserovars in chicken and humans in Lithuania. J Vet Med A. 2006; 53: 12–16.
19.
Hopkins KL, Kirchner M, Guerra B, Granier SA, Lucarelli C, Porreto MC, Jakubczak A, Threlfall EJ, Mevius DJ. Multiresistant Salmonella serovar 4, [5], 12; i;- in Europe: a new pandemic strain? Euro Surveill. 2010: 15: 2–11.
20.
Wasyl D, Hoszowski A. Occurrence and characterization of monophasic Salmonella entericaserovar Typhimurium (1, 4, [5], 12: i: -) of non-human origin in Poland. Foodborne Pathog Dis. 2012; 9; 1037–1043.
21.
Myšková P, Oslanecová L, Drahovská H, Karpíšková R. Clonal distribution of monophasic Salmonella enterica subsp. enterica serotype 4, [5], 12:i:- in Czech Republic. Foodborne Pathog Dis. 2014; 11: 664–666.
22.
Adzitey F, Rusul G, Huda N. Prevalence and antibiotic resistance of Salmonellaserovars in ducks, duck rearing and processing environment in Penang, Malaysia. Food Res Int. 2011; 45: 947–952.
23.
WHO. WHO surveillance programme for control of foodborne infection and intoxication in Europe 8 th report 1999–2000. Country Reports: Latvia. 2000. http://www.bfr.bund.de/interne.... Accessed:1.10.2016.
24.
Bertrand S, Rimhanen-Finne R, Weill FX, Rabsch W, Thornton L, Perevoscikovs J, van Pelt W, Heck M. Salmonella infections associated with reptiles: the current situation in Europe. Euro Surveill. 2008; 13: pii=18902008;13(24):pii=1890.
25.
SPKC. Epidemioloģijasbiļetens. TheCentreforDiseasePreventionand Control. 2015; http: //www.spkc.gov.lv/infekcijas-slimibu-statistika/. Accessed: 22.04.2016.
26.
Kinross P, van Alphen L, Martinez Urtaza J, Struelens M, Takkinen J, Coulombier D, Mäkelä P, Bertrand S, Mattheus W, Schmid D, Kanitz E, Rücker V, Krisztalovics K, Pászti J, Szögyényi Z, Lancz Z, Ravsch W, Pfefferkorn B, Hiller P, Mooijman K, Gossner C. Multidisciplinary investigation of a multicountry outbreak of Salmonella Stanley infections associated with turkey meat in the European Union, August 2011 to January 2013. Euro Surveill. 2014; 19(19): pii=20801.
27.
White DG, Zhao S, Sudler R, Ayers S, Friedman S, Chen S, McDermott PF, McDermott S, Wagner DD, Meng J. The isolation of antibiotic-resistant Salmonella from retail ground meats. N Engl J Med. 2001; 345: 1147–1154.
28.
Mayrhofer S, Paulsen P, Smulders FJM, Hilbert F. Antimicrobial resistance profile of five major food-borne pathogens isolated from beef, pork and poultry. Int J Food Microbiol. 2004; 97: 23–29.
29.
Nogrády N, Gadó I, Tóth Á, Pászti J. Antibiotic resistance and class 1 integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003. Int J Antimicrob Agents. 2005; 26: 126–132.
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