RESEARCH PAPER
Preliminary assessment of usefulness of cELISA test for screening pig and cattle populations for presence of antibodies against Toxoplasma gondii
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1
Department of Parasitology and Invasive Diseases, National Veterinary Research Institute, Pulawy, Poland
 
2
Unit of Zoonoses, Institute of Rural Health, Lublin, Poland
 
 
Ann Agric Environ Med. 2011;18(2):335-339
 
KEYWORDS
ABSTRACT
Serology testing is an appropriate method for the detection of slaughter animals infected with Toxoplasma, which remain one of the main reservoirs of this parasite in the environment. Competitive ELISA (cELISA) in indirect modification was worked out and optimized for detecting antibodies to Toxoplasma gondii in pigs and cattle. Preliminary validation process showed that the sensitivity and specificity of cELISA obtained in pigs was better than in cattle (88.1% and 94.5% vs. 76.9% and 93.4%, respectively). Sera of 861 pigs and 865 cattle were examined with newly worked out cELISA test and modified agglutination test (MAT) (Toxo-Screen DA, bioMérieux, France). In the total of 1,726 examined animal sera, seropositive results were obtained in 15.0% by cELISA (15.4% in pigs and 14.6% in catttle), and in 13.6% by MAT (14.3% in pigs and 12.8% in cattle). Significant differences in percentages of positive results among populations of the studied animals from various areas of Poland were noted. Obtained results showed the usefulness of cELISA for examining sera of slaughter animals (especially pigs). The considerable rates of infection of slaughter animals with T. gondii in the area of research indicate a potential threat to human health.
REFERENCES (31)
1.
Cook AJC, Gilbert RE, Buffolano W, Zufferey J, Petersen E, Jenum PA, Foulon W, Semprini AE, Dunn DT. Sources of toxoplasma infection in pregnant women: European multi centre case – control study. BMJ 2000;321:142-147.
 
2.
Dawson D. Foodborne protozoan parasites. Int Food Microbiol 2005;103:207-227.
 
3.
Dubey JP, Beattie CP. Toxoplasmosis of Animals and Man. Boca Raton, CRC Press Inc, 1988.
 
4.
Paul M. Potential risk factors for Toxoplasma gondii infection in cases with recently acquired toxoplasmosis. Przegl Epidemiol 1998;52:447-454.
 
5.
Tenter AM, Heckeroth, AR, Weiss LM. Toxoplasma gondii: from animals to humans. Int J Parasitol 2000;30:1217-1258.
 
6.
Sroka J, Zwoliński J, Dutkiewicz J. Seroprevalence of Toxoplasma gondii in farm and wild animals from the area of Lublin province. Bull Vet Pulawy 2007;51:535-540.
 
7.
Dubey JP, Hill DE, Jones JL, Hightower AW, Kirkland E, Roberts JM, Marcet PL, Lehman T, Vianna MC, Miska K, Sreekumar C, Kwok OC, Shen SK, Gamble HR. Prevalence of viable Toxoplasma gondii in beef, chicken, and pork from retail meat stores in the United States: risk assessment to consumers. J Parasitol 2005;91:1082-1093.
 
8.
Dubey JP, Murrell KD, Fayer R, Schad GA. Distribution of Toxoplasma gondii tissue cysts in commercial cuts of pork. J Am Vet Med Assoc 1986;188:1035-1037.
 
9.
Dubey JP, Thulliez P, Weigel RM, Andrews CD, Lind P, Powell EC. Sensitivity and specificity of various serologic tests for detection of Toxoplasma gondii infection in naturally infected sows. Am J Vet Res 1995;56:1030-1036.
 
10.
Dubey JP. Long-term persistence of Toxoplasma gondii in tissues of pigs inoculated with T. gondii oocysts and effect of freezing on viability of tissue cysts in pork. Am J Vet Res 1988;49:910-913.
 
11.
Anderson J. Use of monoclonal antibody in a blocking ELISA to detect group specific antibodies to bluetongue virus. J Virol Methods 1984;12:41-48.
 
12.
Mitchell GF, Premier RR, Garcia EG, Hurrell JGR, Chandler HM, Cruise KM, Tapales, FP, Tiu WV. Hybridoma antibody based competitive ELISA in Schistosoma japonicum infection. Am J Trop Med Hyg 1983;32:114-117.
 
13.
Sroka J, Cencek T, Ziomko I, Karamon J, Zwoliński J. Preliminary assessment of ELISA, MAT and LAT for detecting Toxoplasma gondii antibodies in pigs. Bull Vet Inst Pulawy 2008, 4:545-549.
 
14.
Dubey JP, Andrews CD, Lind P, Kwok OCH, Thulliez P, Lunney JK. Antibody responses measured by various serologic tests in pigs orally inoculated with low numbers of Toxoplasma gondii oocysts. Am J Vet Res 1996;57:1733-1737.
 
15.
Dubey JP, Thulliez P, Powell EC. Toxoplasma gondii in Iowa sows: comparison of antibody titers to isolation of T. gondii by bioassays in mice and cats. J Parasitol 1995;81:48-53.
 
16.
Dubey JP.Validation of the specificity of the modified agglutination test for toxoplasmosis in pigs. Vet Parasitol 1997;71:307-310.
 
17.
Kijlstra A, Jongert E. Control of the risk of human toxoplasmosis transmitted by meat. Int J Parasitol 2008;38:1359-1370.
 
18.
Villari S, Vesco G, Petersen E, Crispo A, Buffolano W. Risk factors for toxoplasmosis in pigs bred in Sicily, Southern Italy. Vet Parasitol 2009;161:1-8.
 
19.
Dubey JP. A review of toxoplasmosis in cattle. Vet Parasitol 1986;22:177-202.
 
20.
Dubey JP. Toxoplasmosis in pigs – the last 20 years. Vet Parasitol 2009;164:89-103.
 
21.
Hill DE, Chirukandoth S, Dubey JP. Biology and epidemiology of Toxoplasma gondii in man and animals. Anim Health Res Rev 2005;6:41-61.
 
22.
Sroka J. Seroepidemiology of toxoplasmosis in the Lublin region. Ann Agric Environ Med 2001;8:25-31.
 
23.
Pawłowski ZS. Toxoplasmosis in Poznań region, Poland 1990-2000. Przegl Epidemiol 2002;56:409-417.
 
24.
Ramisz A, Zemburowa K. Serological survey of Toxoplasma antibodies in animal livestock. Proceedings of the 4th International Congress of Parasitology, August 1978, Warsaw, Poland, p. 85E.
 
25.
Umiński J, Krupa K, Cisak E, Badora J, Wróblewska A. Studies on animal reservoir of toxoplasmosis. Materiały Naukowe XI Zjazdu Polskiego Towarzystwa Epidemiologów i Lekarzy Chorób Zakaźnych, 16-18 September 1988, Puławy, Poland, pp. 37-40.
 
26.
Krupa K, Bartoszcze M. Badania nad występowaniem toksoplazmozy u świń. Profi laktyka antropozoonoz. Lekarz Wojskowy 1991;3-4:186-190.
 
27.
Buhr de K, Ludewig M, Fehlhaber K. Toxoplasma gondii-seroprevalence current results in German swine herds. Arch Lebensmittelhygiene 2008;59:5-8.
 
28.
Giessen van der J, Fonville M, Bouwknegt M, Langelaar M, Vollema A: Seroprevalence of Trichinella spiralis and Toxoplasma gondii in pigs from different housing systems in The Netherlands. Vet Parasitol 2007;148:371-374.
 
29.
Kijlstra A, Meerburg B, Cornelissen J, De Craeye S, Vereijken P, Jongert E. The role of rodents and shrews in the transmission of Toxoplasma gondii to pigs. Vet Parasitol 2008;156:183-190.
 
30.
Klun I, Djurkovic–Djakovic O, Katic-Radivojevic S, Nikolic A. Cross-sectional survey on Toxoplasma gondii infection in cattle, sheep and pigs in Serbia: Seroprevalence and risk factors. Vet Parasitol 2006;135:121-131.
 
31.
Panadero R, Painceira A, López C, Vázquez L, Paz A, Díaz P, Dacal V, Cienfuegos S, Fernández G, Lago N, Díez-Bańos P, Morrondo P. Seroprevalence of Toxoplasma gondii and Neospora caninum in wild and domestic ruminants sharing pastures in Galicia (Northwest Spain). Res Vet Sci 2010;88:111-115.
 
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