RESEARCH PAPER
Melatonin reverses the enhanced oxidative damage to membrane lipids and improves skin biophysical characteristics in former-smokers – A study in postmenopausal women
 
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1
Department of Oncological Endocrinology, Medical University of Lodz, Lodz, Poland
 
2
Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, Lodz, Poland
 
3
Polish Mother’s Memorial Hospital – Research Institute, Lodz, Poland
 
 
Corresponding author
Malgorzata Karbownik-Lewinska   

Department of Oncological Endocrinology, Medical University of Lodz, Lodz, Poland
 
 
Ann Agric Environ Med. 2017;24(4):659-666
 
KEYWORDS
ABSTRACT
Introduction and objective:
Protective antioxidative effects of melatonin have been repeatedly documented in experimental and clinical studies. One of the most spectacular exogenous prooxidative agents is cigarette smoking. The aim of the study was to evaluate the level of oxidative damage to membrane lipids (lipid peroxidation; LPO) in blood serum, and in epidermis exfoliated during microdermabrasion collected from former-smokers who were treated with melatonin.

Material and Methods:
The study was performed in postmenopausal women. Ninety (90) female volunteers, aged 46–67 years, were enrolled. Two major groups, i.e. never-smokers (n=44) and former-smokers (n=46), were divided into: Control, melatonin topical skin application, Restructurer (containing antioxidants) topical skin application, and melatonin oral treatment. Microdermabrasion was performed at point ‘0’, after 2 weeks, and after 4 weeks of treatment. The following parameters were measured: LPO in blood serum, LPO in epidermis exfoliated during microdermabrasion, and skin biophysical characteristics, such as sebum, moisture, elasticity, and pigmentation. Malondialdehyde+4-hydroxyalkenals level (LPO index) was measured spectrophotometrically.

Results:
Melatonin oral treatment significantly reversed the increased serum LPO level in former-smokers already after 2 weeks of treatment. In a univariate regression model, LPO blood level constituted the only independent factor negatively associated with melatonin oral treatment. After 4 weeks of treatment, melatonin given orally increased skin sebum, moisture and elasticity levels, and melatonin applied topically increased sebum level.

Conclusions:
Exogenous melatonin reverses the enhanced oxidative damage to membrane lipids and improves skin biophysical characteristics in former-smokers.

 
REFERENCES (36)
1.
Tan DX, Manchester LC, Esteban-Zubero E, Zhou Z, Reiter RJ. Melatonin as a Potent and Inducible Endogenous Antioxidant: Synthesis and Metabolism. Molecules 2015; 20(10): 18886–18906.
 
2.
Manchester LC, Coto-Montes A, Boga JA, Andersen LP, Zhou Z, Galano A, Vriend J, Tan DX, Reiter RJ. Melatonin: an ancient molecule that makes oxygen metabolically tolerable. J Pineal Res. 2015; 59(4): 403–419.
 
3.
Karbownik M, Lewinski A, Reiter RJ. Anticarcinogenic actions of melatonin which involve antioxidative processes: comparison with other antioxidants. Int J Biochem Cell Biol. 2001; 33(8): 735–753.
 
4.
García JJ, López-Pingarrón L, Almeida-Souza P, Tres A, Escudero P, García-Gil FA, Tan DX, Reiter RJ, Ramírez JM, Bernal-Pérez M. Protective effects of melatonin in reducing oxidative stress and in preserving the fluidity of biological membranes: a review. J Pineal Res. 2014; 56(3): 225–37.
 
5.
Janjetovic Z, Nahmias ZP, Hanna S, Jarrett SG, Kim TK, Reiter RJ, Slominski AT. Melatonin and its metabolites ameliorate ultraviolet B-induced damage in human epidermal keratinocytes. J Pineal Res. 2014; 57(1): 90–102.
 
6.
Kleszczyński K, Zwicker S, Tukaj S, Kasperkiewicz M, Zillikens D, Wolf R, Fischer TW. Melatonin compensates silencing of heat shock protein 70 and suppresses ultraviolet radiation-induced inflammation in human skin ex vivo and cultured keratinocytes. J Pineal Res. 2015; 58(1): 117–126.
 
7.
Slominski AT, Kleszczyński K, Semak I, Janjetovic Z, Zmijewski MA, Kim TK, Slominski RM, Reiter RJ, Fischer TW. Local melatoninergic system as the protector of skin integrity. Int J Mol Sci. 2014; 15(10): 17705–17732.
 
8.
Karbownik M, Lewinski A. Melatonin reduces Fenton reaction-induced lipid peroxidation in porcine thyroid tissue. J Cell Biochem. 2003; 90(4): 806–811.
 
9.
Karbownik-Lewinska M, Gesing A, Zasada K, Jedrzejczyk M, Sobieszczanska-Jablonska A, Krawczyk J, et al. Relationship between lipid peroxidation or carcinoembryonic antigen and risk factors for non-communicable diseases in women at midlife and beyond. Neuro Endocrinol Lett. 2012; 33(5): 536–545.
 
10.
Karbownik-Lewinska M, Kokoszko A, Lewandowski K, Shalet SM, Lewinski A. Growth hormone replacement reduces increased lipid peroxidation in growth hormone-deficient adults. Clin Endocrinol. 2008; 68: 957–964.
 
11.
Karbownik-Lewinska M, Szosland J, Kokoszko-Bilska A, Stępniak J, Zasada K, Gesing A, et al. Direct contribution of obesity to oxidative damage to macromolecules. Neuro Endocrinol Lett. 2012; 33(4): 453–461.
 
12.
Lewandowski KC, Stojanovic N, Press M, Tuck S, Lewiński A, Karbownik-Lewińska M. Raised concentrations of lipid peroxidation products (LPO) in pregnant women with impaired glucose tolerance. Ann Agric Environ Med. 2014; 21(2): 429–434.
 
13.
Milczarek M, Stępniak J, Lewiński A, Karbownik-Lewińska M. Potassium iodide, but not potassium iodate, as a potential protective agent against oxidative damage to membrane lipids in porcine thyroid. Thyroid Res. 2013; 6(1): 10.
 
14.
Stępniak J, Lewiński A, Karbownik-Lewińska M. Membrane lipids and nuclear DNA are differently susceptive to Fenton reaction substrates in porcine thyroid. Toxicol In Vitro. 2013; 27(1): 71–78.
 
15.
Szychta P, Zadrozny M, Lewinski A, Karbownik-Lewinska M. Increased oxidative damage to membrane lipids following surgery for breast cancer. Neuro Endocrinol Lett. 2014; 35(7): 602–607.
 
16.
Domej W, Oettl K, Renner W. Oxidative stress and free radicals in COPD--implications and relevance for treatment. Int J Chron Obstruct Pulmon Dis. 2014; 9: 1207–1224.
 
17.
Goldkorn T, Filosto S, Chung S. Lung injury and lung cancer caused by cigarette smoke-induced oxidative stress: Molecular mechanisms and therapeutic opportunities involving the ceramide-generating machinery and epidermal growth factor receptor. Antioxid Redox Signal. 2014; 21(15): 2149–2174.
 
18.
Siasos G, Tsigkou V, Kokkou E, Oikonomou E, Vavuranakis M, Vlachopoulos C, et al. Smoking and atherosclerosis: mechanisms of disease and new therapeutic approaches. Curr Med Chem. 2014; 21(34): 3936–3948.
 
19.
Hakim IA, Harris R, Garland L, Cordova CA, Mikhael DM, Sherry Chow HH. Gender difference in systemic oxidative stress and antioxidant capacity in current and former heavy smokers. Cancer Epidemiol Biomarkers Prev. 2012; 21(12): 2193–2200.
 
20.
Haswell LE, Papadopoulou E, Newland N, Shepperd CJ, Lowe FJ. A cross-sectional analysis of candidate biomarkers of biological effect in smokers, never-smokers and ex-smokers. Biomarkers. 2014; 19(5): 356–367.
 
21.
Sagan D, Stepniak J, Gesing A, Lewinski A, Karbownik-Lewinska M. Higher lipid peroxidation in former-smokers vs.never-smokers – study in postmenopausal women. Neuro Endocrinol Lett. 2015; 36(6): 101–107.
 
22.
Szokalska K, Stepniak J, Karbownik-Lewinska M. Lipid peroxidation evaluated in epidermis exfoliated during microdermabrasion is a reliable marker of oxidative stress related to obesity. J Eur Acad Dermatol Venereol. 2015 in print.
 
23.
Kasperczyk S, Dobrakowski M, Kasperczyk A, Zalejska-Fiolka J, Pawlas N, Kapka-Skrzypczak L, Birkner E. Effect of treatment with N-acetylcysteine on non-enzymatic antioxidant reserves and lipid peroxidation in workers exposed to lead. Ann Agric Environ Med. 2014; 21(2): 272–277.
 
24.
Krzeszowiak J, Zawadzki M, Markiewicz-Górka I, Kawalec A, Pawlas K. The influence of 9-day trekking in the Alps on the level of oxidative stress parameters and blood parameters in native lowlanders. Ann Agric Environ Med. 2014; 21(3): 585–589.
 
25.
Łuczaj W, Moniuszko A, Rusak M, Zajkowska J, Pancewicz S, Skrzydlewska E. Peroxidative metabolism of arachidonic acid in the course of Lyme arthritis. Ann Agric Environ Med. 2015; 22(3): 433–437.
 
26.
Kirkland EB, Hantash BM. Microdermabrasion: molecular mechanisms unraveled, part 1. J Drugs Dermatol. 2012; 11: 2–9.
 
27.
Freedman BM. Topical antioxidant application enhances the effects of facial microdermabrasion. J Dermatolog Treat. 2009; 20(2): 82–87.
 
28.
Karbownik M, Tan D, Manchester LC, Reiter RJ. Renal toxicity of the carcinogen delta-aminolevulinic acid: antioxidant effects of melatonin. Cancer Lett. 2000; 161(1): 1–7.
 
29.
Karbownik M, Stasiak M, Zygmunt A, Zasada K, Lewiński A. Protective effects of melatonin and indole-3-propionic acid against lipid peroxidation, caused by potassium bromate in the rat kidney. Cell Biochem Funct. 2006; 24(6): 483–489.
 
30.
Karbownik M, Reiter RJ. Melatonin protects against oxidative stress caused by delta-aminolevulinic acid: implications for cancer reduction. Cancer Invest. 2002; 20(2): 276–286.
 
31.
Gitto E, Karbownik M, Reiter RJ, Tan DX, Cuzzocrea S, Chiurazzi P, Cordaro S, Corona G, Trimarchi G, Barberi I. Effects of melatonin treatment in septic newborns. Pediatr Res. 2001; 50(6): 756–60.
 
32.
Shin IS, Shin NR, Park JW, Jeon CM, Hong JM, Kwon OK, Kim JS, Lee IC, Kim JC, Oh SR, Ahn KS. Melatonin attenuates neutrophil inflammation and mucus secretion in cigarette smoke-induced chronic obstructive pulmonary diseases via the suppression of Erk-Sp1 signaling. J Pineal Res. 2015; 58(1): 50–60.
 
33.
Bejarano I, Monllor F, Marchena AM, Ortiz A, Lozano G, Jiménez MI, Gaspar P, García JF, Pariente JA, Rodríguez AB, Espino J. Exogenous melatonin supplementation prevents oxidative stress-evoked DNA damage in human spermatozoa. J Pineal Res. 2014; 57(3): 333–339.
 
34.
Yang GH, Li YC, Wang ZQ, Liu B, Ye W, Ni L, Zeng R, Miao SY, Wang LF, Liu CW. Protective effect of melatonin on cigarette smoke-induced restenosis in rat carotid arteries after balloon injury. J Pineal Res. 2014; 57(4): 451–458.
 
35.
Tarnoki AD, Littvay L, Lazar Z, Karlinger K, Molnar AA, Melicher D, Garami Z, Berczi V, Horvath I. Transmission of second-hand smoke sensitivity and smoking attTarnoki DLitude in a family. Ann Agric Environ Med. 2014; 21(4): 771–5.
 
36.
Andersen LP, Gögenur I, Rosenberg J, Reiter RJ. The Safety of Melatonin in Humans. Clin Drug Investig 2015 in print.
 
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