RESEARCH PAPER
In vitro evaluation of the effect of tobacco smoke on rat cornea function
| 1 | Department of Bionics and Bioimpedance, University of Medical Sciences, Poznań, Poland |
| 2 | Department of Physiology, University of Medical Sciences, Poznań, Poland |
| 3 | Institute of Rural Health in Lublin, Lublin, Poland |
| 4 | Department of Physiotherapy, Rheumatology and Rehabilitation, University of Medical Sciences, Poznań, Poland |
| 5 | Laboratory of Environmental Research, Department of Toxicology, Poznań University of Medical Sciences, Poznań, Poland |
CORRESPONDING AUTHOR
Ann Agric Environ Med. 2012;19(4):677–679
KEYWORDS
ABSTRACT
The influence of tobacco smoke on the dielectric properties of rat cornea were measured in vitro over the frequency range of the electric field of 500Hz–100kHz and in temperatures of the air from 25 to 150°C. The temperature dependencies of the loss tangent for both healthy and smoky cornea represent the relation between the energy lost and the energy stored in the epithelium-stromal-endothelium systems of the cornea. The differences between the healthy and the smoky cornea concerned the temperature ranges in which there appeared the decomposition of loosely-bound water and β-relaxation associated with polar side-chains relaxations on protein molecules of this tissue. The effect of smoke is manifested as a shift of the loss tangent peaks of these two processes towards higher temperatures, when compared with the control. The results are interpreted as caused by the toxic compounds of the tobacco smoke leading to higher ion transport in the nonhomogeneous structure of the cornea when compared to that of the control tissue. The activation energy of conductivity were similar for the healthy and smoky cornea as a consequence of the braking of hydrogen and Van der Waals bonds between loosely bound water, and the proteins of channels in the epithelium and endothelium. Recognition of the effect of frequency and temperature on the dielectric behaviour of the smoky cornea may be of interest for disease characterization of this tissue.
REFERENCES (16)
1.
Marzec E, Olszewski J. Dielectric analysis of the thermal processes of human nail. Thermochim Acta 2010; 506: 73-76.
2.
Pethig R. Dielectric and Electronic Properties of Biological Materials. Wiley, Chichester, 1979.
3.
Pietrucha K, Marzec E. Dielectric properties of the collagen-glycosaminoglycans scaffolds in the temperature range of thermal decomposition. Biophys Chem. 2005; 118: 51-56.
4.
Drexler JW, Powell HM. Dehydrothermal crosslinking of electrospun collagen. Tissue Eng Part C-Me. 2011; 17: 9-17.
5.
Figueiro SD, Mallmann EJJ, Goes JC, Ricardo NMPS, Denardin JC, Sombra ASB, Fechine PBA. New ferrimagnetic biocomposite film based in collagen and yttrium iron garnet. Express Polymer Letters 2010; 4: 790-797.
6.
Chetoni P, Burgalassi S, Monti D, Saettone MF. Ocular toxicity of some corneal penetration enhancers evaluated by electrophysiology measurements on isolated rabbit corneas. Toxicology in Vitro 2003; 17: 497-504.
7.
Mergler S, Pleyer U. The human corneal endothelium: New insights into electrophysiology and ion channels. Prog Retin Eye Res. 2007; 26: 359-378.
8.
Regini JW, Elliott GF, Hodson SA. The ordering of corneal collagen fibrils with increasing ionic strength. J Mol Biol. 2004; 336: 179-186.
9.
Guimera A, Ivorra A, Gabriel G, Villa R. Non-invasive assessment of corneal endothelial permeability by means of electrical impedance measurements. Med Eng Phys. 2010; 32: 1107-1115.
10.
Woodward MA, Edelhauser HF. Corneal endothelium after refractive surgery. J Cataract Refract Surg. 2011; 37: 767-777.
11.
Grus FH, Sabuncuo P, Augustin A, Pfeiffer N. Effect of smoking on tear proteins. Graefe’s Arch Clin Exp Ophthalmol. 2002; 240: 889-892.
13.
Rummenie VT, Matsumoto Y, Dogru M, Wang Y, Hu Y, Ward SK, et al. Tear cytokine and ocular surface alternations following brief passive cigarette smoke exposure. Cytokine 2008; 43: 200-208.
14.
van der Vaart H, Postma DS, Timens W, Ten Hacken NH. Acute effects of cigarette smoke on inflammation and oxidative stress a review. orax. 2004; 59: 713-721.
15.
Wollensak G, Spoerl E. Collagen crosslinking of human and porcine sclera. J Cataract Refract Surg. 2004; 30: 689-695.
16.
Olszewski J, Marzec E, Kulza M, Samborski W. Implications of the alpha dispersion for studies on interaction of tobacco smoke – corneal tissue. Colloids Surf B: Biointerfaces. 2012; 90: 109-112.
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
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