First report of Enterocytozoon bieneusi and Encephalitozoon intestinalis infection of wild mice in Slovakia
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Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
Department of Zoology, Slovak Academy of Science, Košice, Slovak Republic
Ann Agric Environ Med. 2015;22(2):251-252
Increased risk of zoonotic transmission of the potential human pathogenic species Enterocytozoon bieneusi, Encephalitozoon intestinalis and Encephalitozoon cuniculi was detected in wild immunocompetent mice (Mus musculus musculus; n=280). Analysis was conducted with the use of PMP1/PMP2 primers and SYBR Green RT-PCR. Using Real Time PCR and comparing the sequences with sequences in the GenBank, E. bieneusi was detected in 3 samples (1.07 %), E. cuniculi in 1 sample (0.35 %) and E. intestinalis in 1 sample (0.35 %). The results of this report document the low host specificity of detected microsporidia species, and imply the importance of synanthropic rodents as a potential source of human microsporidial infection.
The study has supported by the Grant VEGA, No. 1/0390/12, 1/0063/13.
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