RESEARCH PAPER
Characterization of Polish feline B. henselae isolates by multiple-locus tandem repeat analysis and pulse-field gel electrophoresis
 
More details
Hide details
1
National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland
 
2
UMR BIPAR//ENVA/AFSSA/UVPM USC INRA, Maisons-Alfort, France
 
 
Corresponding author
Edyta Podsiadly   

National Institute of Public Health - National Institute of Hygiene, Warsaw, Poland
 
 
Ann Agric Environ Med. 2012;19(1):39-43
 
KEYWORDS
ABSTRACT
Knowledge about molecular epidemiology of B. henselae is important for recognizing the geographical distribution of strains and identification of isolates virulent for humans. Eleven Polish feline B. henselae isolates were typed, using 2 different techniques: pulse-field gel electrophoresis (PFGE) and multiple-locus variable-number tandem repeat analysis (MLVA). PFGE analysis distinguished 6 different PFGE types, with subtypes within 3 of them, whereas 10 MLVA types were assigned. Global diversity index (D.I.) for MLVA equaled 0.93. For 7 isolates, the results of MLVA confirmed cluster assignments based on PFGE. Both PFGE and MLVA results were in accordance with epidemiological data. Although PFGE has been previously demonstrated to be a suitable method for the differentiation of B. henselae isolates/strains, our results show the superiority of MLVA over PFGE with respect to higher discriminatory power, distinguishing genotypes I and II isolates, easier analysis of results, and possibility to compare the numerical data obtained by different laboratories. With MLVA, 7 new profiles were observed, compared to previous results from around the world; whereas 3 known profiles were previously described mainly in European B. henselae isolates. Our results confirm that some VNTR profiles can be used as specific geographical markers.
REFERENCES (20)
1.
Chomel BB, Boulouis H-J, Maruyama S Breitschwerdt EB. Bartonella spp. in pets and effect on human health. Emerg Infec Dis. 2006; 12: 389-394.
 
2.
Boulouis HJ, Chang CC, Henn JB, Kasten RW Chomel BB. Factors associated with the rapid emergence of zoonotic Bartonella infections. Vet Res. 2005; 36: 383-410.
 
3.
Podsiadly E, Chmielewski T, Marczak R, Sochon E, Tylewska-Wierzbanowska S. Bartonella henselae in the human environment in Poland. Scan J Infec Dis. 2007; 39: 956-962.
 
4.
Bergmans AM, Schellekens J, van Embden J, Schouls LM. Predominance of two Bartonella henselae variants among cat scratch disease patients in the Netherlands. J Clin Microbiol. 1996; 34: 254-60.
 
5.
Drancourt M, Birtles R, Chaumentin G, Vandenesch F, Etienne J, Raoult D. New serotype of Bartonella henselae in endocarditis and cat-scratch disease. Lancet 1996; 17: 441-443.
 
6.
Zeaiter Z, Fournier PE, Raoult D. Genomic variation of Bartonella henselae strains detected in lymph nodes from patients with cat-scratch disease. J Clin Microbiol. 2002; 40: 1023-1030.
 
7.
Arvand M, Feil EJ, Gladi M, Boulouis H-J, Viezens J. Multi-Locus Sequence Typing of Bartonella henselae isolates from three continents reveals hypervirulent and feline associated clones. PLoS ONE 2007; 12: e1346.
 
8.
Maruyama S, Kasten RW, Boulouis HJ, Gurfield NA, Katsube Y, Chomel BB. Genomic diversity of Bartonella henselae isolates from domestic cats from Japan, the USA and France by pulsed-field gel electrophoresis. Vet Microbiol. 2001; 79: 337-349.
 
9.
Dillon B, Valenzuela J, Don R, Blanckenberg D, Wigney DI, Malik R, Morris AJ, Robson JM Iredell J. Limited diversity among human isolates of Bartonella henselae. J Clin Microbiol. 2002; 40: 4691-4699.
 
10.
Iredell J, Blanckenberg D, Arvand M, Grauling S, Feil EJ, Birtles RJ. Characterization of the natural population of Bartonella henselae by Multilocus Sequence Typing. J Clin Microbiol. 2003; 41: 5071-5079.
 
11.
Li W, Chomel BB, Maruyama S, Guptill L, Sander A, Raoult D, Fournier PE. Multispacer typing to study the genotypic distribution of Bartonella henselae populations. J Clin Microbiol. 2006; 44: 2499-506.
 
12.
Monteil M, Durand B, Bouchouicha R, Petit E, Chomel B, Arvand M, Boulouis HJ Haddad N. Development of discriminatory multiple locus variable number tandem repeat analysis for Bartonella henselae. Microbiology 2007; 153: 1141-1148.
 
13.
Tenover FC, Arbeit RD, Goering RV, Mickelsen PA, Murray BE, Persing DH Swaminathan B. Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol. 1995; 33: 2233-2239.
 
14.
Hunter PR, Gaston MA: Numerical index of the discriminatory ability of typing systems: an application of Simpson’s index of diversity. J Clin Microbiol. 1988; 26: 2465-2466.
 
15.
Bouchouicha R, Durand B, Monteli M, Chomel BB, Berrich M, Arvand M, Brites RJ, Breitschwerdt EB, Koehler JE, Maggi R, Maruyama S, Kasten R, Petit E, Boulouis H-J Haddad N. Molecular epidemiology of feline and human Bartonella henselae isolates. Emerg Infec Dis. 2009; 15: 813-816.
 
16.
Bouchouicha R, Boulouis H-J, Berrich M, Monteil M, Chomel B, Haddad N. Comparison of performances of MLVA vs. the main other typing techniques for Bartonella henselae. Clin Microbiol Infec. 2009; Suppl 2: 104-5.
 
17.
Sander A, Ruess M, Bereswill S, Schuppler M Steinbrueckner B. Comparison of different DNA fingerprinting techniques for molecular typing of Bartonella henselae isolates. J Clin Microbiol. 1998; 36: 2973-2981.
 
18.
Xu C, Liu Q, Diao B, Kan B, Song X, Li D. Optimization of pulse-field gel electrophoresis for Bartonella subtyping. J Microbiol Methods. 2009; 76: 6-11.
 
19.
Chang CC, Chomel BB, Kasten RW, Tappero JW, Sanchez MA, Koehler JE. Molecular epidemiology of Bartonella henselae infection in human immunodeficiency virus-infected patients and their cat contacts, using pulsed-field gel electrophoresis and genotyping. J Infect Dis. 2002; 186: 1733-9.
 
20.
Sander A, Posselt M, Bohm N, Ruess M Altwegg M. Detection of Bartonella henselae DNA by two different PCR assays and determination of the genotypes of strains involved in histologically defined cat scratch disease. J Clin Microbiol. 1999; 37: 993-997.
 
eISSN:1898-2263
ISSN:1232-1966
Journals System - logo
Scroll to top