Mine blast injuries are characterized by multiple blood vessel damage, as well as damage to nerve fibres, muscles and skin. Infection is an additional aggravating factor. One treatment method is cell therapy. A promising direction of this technique is platelet autocryolysate (PAC), an abiotechnological product based on the oligopeptides and growth factors obtained from the patient’s blood platelets. A patient was treated who had suffered a long-term non-healing mine blast wound of the soft tissues of the left leg. Two secondary surgical debridements were performed, followed by vacuum therapy. The inflammatory process intensified and was complicated by phlebothrombosis of the deep veins of the leg. After two applications of PAC, the wound size decreased, the discharge disappeared, and the wound surface was covered with a dry, loose crust, under which active epithelialization occurred. Using PAС has a positive therapeutic effect in treating long-term, non-healing blast wounds of the tissues.