RESEARCH PAPER
Analysis of novel serum markers of fibrosis and angiogenesis in patients with alcoholic liver
cirrhosis
1
Department of Internal Medicine, Medical University, Lublin, Poland
2
Department of Experimental Haematooncology, Medical University, Lublin, Poland
3
Department of Medical Chemistry, Medical University, Lublin, Poland
4
Department of Family Medicine and Community Nursing, Medical University, Lublin, Poland
5
Department of Internal Diseases and Hypertension, Institute of Rural Health, Lublin, Poland
Corresponding author
Ann Agric Environ Med. 2020;27(4):568-573
KEYWORDS
alcoholic liver cirrhosisangiopoietin-like peptide 4 (ANGPTL-4)asialoglycoprotein receptor 1 (ASGP-R1)S100 calcium-binding protein A8 (S100A8)hyaluronic acid (Hyal)collagen IV (Coll IV)
TOPICS
ABSTRACT
Introduction:
Alcohol consumption causes acute and chronic liver injury. The clinical forms of alcohol liver disease (ALD) include steatosis, hepatitis, cirrhosis, and hepatocellular carcinoma (HCC) associated with liver cirrhosis.
Objective:
The aim of the study was to determine the levels of novel markers of fibrogenesis and angiogenesis in patients with alcoholic liver cirrhosis. Serum levels of angiopoietin-like peptide 4 (ANGPTL-4), asialoglycoprotein receptor 1 (ASGP-R1), and S100 calcium-binding protein A8 (S100A8) were assessed. Levels of hyaluronic acid (Hyal) and collagen IV (Coll IV) werealso determined at various stages of alcoholic liver cirrhosis.
Material and methods:
The study group consisted of 72 patients with alcoholic liver cirrhosis, while the control group included 22 healthy subjects without a history of alcohol abuse. The degree of liver cirrhosis was evaluated according to the Pugh-Child criteria (Pugh-Child score). Based on thse scores, patients were assigned to one of three groups: Pugh-Child (P-Ch) A – 21 with stage A, P-Ch B – 23 with stage B and P-Ch C – 28 with stage C liver cirrhosis. Serum levels of markers were determined using ELISA.
Results:
The study findings demonstrated higher levels of ANGPTL-4, ASGP-R1, S100A, hyaluronic acid and serum collagen IV in the group of patients with alcoholic liver cirrhosis, compared to the control group. Furthermore, their levels increased with the progression of alcoholic liver cirrhosis.
Conclusions:
The biomarkers analysed in the study may be useful for diagnosis and prognosis in patients with alcoholic liver cirrhosis.
Alcohol consumption causes acute and chronic liver injury. The clinical forms of alcohol liver disease (ALD) include steatosis, hepatitis, cirrhosis, and hepatocellular carcinoma (HCC) associated with liver cirrhosis.
Objective:
The aim of the study was to determine the levels of novel markers of fibrogenesis and angiogenesis in patients with alcoholic liver cirrhosis. Serum levels of angiopoietin-like peptide 4 (ANGPTL-4), asialoglycoprotein receptor 1 (ASGP-R1), and S100 calcium-binding protein A8 (S100A8) were assessed. Levels of hyaluronic acid (Hyal) and collagen IV (Coll IV) werealso determined at various stages of alcoholic liver cirrhosis.
Material and methods:
The study group consisted of 72 patients with alcoholic liver cirrhosis, while the control group included 22 healthy subjects without a history of alcohol abuse. The degree of liver cirrhosis was evaluated according to the Pugh-Child criteria (Pugh-Child score). Based on thse scores, patients were assigned to one of three groups: Pugh-Child (P-Ch) A – 21 with stage A, P-Ch B – 23 with stage B and P-Ch C – 28 with stage C liver cirrhosis. Serum levels of markers were determined using ELISA.
Results:
The study findings demonstrated higher levels of ANGPTL-4, ASGP-R1, S100A, hyaluronic acid and serum collagen IV in the group of patients with alcoholic liver cirrhosis, compared to the control group. Furthermore, their levels increased with the progression of alcoholic liver cirrhosis.
Conclusions:
The biomarkers analysed in the study may be useful for diagnosis and prognosis in patients with alcoholic liver cirrhosis.
ACKNOWLEDGEMENTS
This study has been performed at Medical University of Lublin, Poland, and it was funded by a Grant from the Medical University of Lublin (DS 507/2013–2015). The authors thank Anna Misiuna, who provided medical writing services on behalf of Medical University of Lublin, Poland.
Prystupa A, Kiciński P, Luchowska-Kocot D, Nowicki G, Dzida G, Myśliński W, Zakrzewski M, Mosiewicz J, Panasiuk L. Analysis of novel
serum markers of fibrosis and angiogenesis in patients with alcoholic liver cirrhosis. Ann Agric Environ Med. 2020; 27(4): 568–573.
doi: 10.26444/aaem/127621
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| eISSN: | 1898-2263 |
| ISSN: | 1232-1966 |
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