A 56-year-old man with RT-PCR negative nasopharyngeal swabs with Coronavirus Disease 2019 (COVID-19) Pneumonia
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Chair and Department of Infectious Diseases, Medical University, Lublin, Poland
Chair and Department of Internal Diseases, Medical University, Lublin, Poland
Institute of Rural Health, Lublin, Poland
Corresponding author
Anna Dworzańska   

Medical University of Lublin Chair and Department of Infectious Diseases, Staszica 16, 20-081, Lublin, Poland
Ann Agric Environ Med. 2020;27(2):317-318
Diagnostic procedure in Coronavirus Disease 2019 (COVID-19) is based mainly on performing real-time-reverse transcription-polymerase chain-reaction (RT-PCR), which has been accepted as the gold standard method. In some cases, such as mutations of the SARS-CoV-2 genome, variable viral load kinetics or laboratory errors, it can be false-negative.

Case report:
The case is presented of a 56-year-old man with respiratory tract symptoms, with twice negative results of real-time-reverse transcription-polymerase chain-reaction of nasopharyngeal swabs and positive chest computed tomography, with typical findings for COVID-19 pneumonia.

Patients with negative RT-PCR results, but with positive computed tomography findings characteristic for COVID-19, should be treated as well as those infected.

Gorbalenya AE, Baker SC, Baric RS, et al. Severe acute respiratory syndrome related coronavirus: The species and its viruses a statement of the Coronavirus Study Group. Microbiology. 2020 Feb 19. doi: /10.1101/2020.02.07.937862.
Centers for Disease Control and Prevention (CDC). Coronavirus Disease 2019 (COVID-19). Atlanta; 2020. Available from
Guang Chen, Di Wu, Wei Guo, et al. Clinical and immunologic features in severe and moderate forms of Coronavirus Disease 2019. J Clin Invest. 2020 Apr 12. doi: 10.1172/JCI137244.
Wang X, Tan L, Wang X, et al. Comparison of nasopharyngeal and oropharyngeal swabs for SARS-CoV-2 detection in 353 patients received tests with both specimens simultaneously. Int J Infect Dis. 2020 Apr 94: 107–109.
Fang Y, Zhang H, Xie J, et al. Sensitivity of Chest CT for COVID-19: Comparison to RT-PCR. Radiology. 2020 Feb 19. doi: 10.1148/radiol.2020200432.
Tahamtan A, Abdollah A. Real-time RT-PCR in COVID-19 detection: issues affecting the results. Expert Rev Mol Diagn. 2020 Apr 22: 1–2. doi: 10.1080/14737159.2020.1757437.
Phan T. Genetic diveristy andevolution of SARS-CoV-2. Infect Genet Evol. 2020 Feb 21(81): 104260.
Xie X, Zhong Z, Zhao W, et al. Chest CT for Typical 2019-nCoV Pneumonia: Relationship to Negative RT-PCR Testing. Radiology. 2020 Feb 12. doi: 10.1148/radiol.2020200343.
Huang P, Liu T, Huang L, et al. Use of chest CT in combination with negative RT-PCR assay for the 2019 novel coronavirus but high clinical suspicion. Radiology. 2020 Feb 12. doi: org/10.1148/radiol.2020200330.
Tao Ai, Yang Z, Hou H, et al. Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 Cases. Radiology. 2020 Feb 26. doi: 10.1148/radiol.2020200642.
Jiang GM, Ren X, Liu Y, et al. Application and optimization of RT-PCR in diagnosis of SARS-CoV-2-infection. Lancet Infect Dis. 2020 Feb. doi: 10.1101/2020.02.25.20027755.
Bai HX, Hsieh B, Xiong Z, et al. Performance of Radiologists in Differentiating COVID-19 From Viral Pneumonia on Chest CT. Radiology. 2020 Mar 10. 200823. doi: 10.1148/radiol.2020200823.
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