RESEARCH PAPER
Aeromonas hydrophila subsp. dhakensis – a causative agent of gastroenteritis imported into the Czech Republic
1
Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic
2
Institute of Public Health, Centre for Clinical Laboratories, Ostrava, Czech Republic
Corresponding author
Ivo Sedláček
Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic
Czech Collection of Microorganisms, Department of Experimental Biology, Masaryk University, Brno, Czech Republic
Ann Agric Environ Med. 2012;19(3):409–413
KEYWORDS
ABSTRACT
Out of the twenty-one A. hydrophila complex isolates obtained during a routine examination of human diarrhoeal faeces, two A. hydrophila subsp. dhakensis isolates (P1097 = CCM 7329 and P1165) were successfully identified by ribotyping. The correct taxonomic position of the A. hydrophila subsp. dhakensis CCM 7329 was verified by cpn60 sequencing (GeneBank accession number HM536193). The remaining A. hydrophila complex isolates were identified as A. hydrophila subsp. hydrophila. The ability of biochemical tests and fatty acid methyl ester analysis to reliably discern both A. hydrophila subsp. dhakensis and A. hydrophila subsp. hydrophila was limited. In contrast to the A. hydrophila subsp.hydrophila, the faecal isolates of A. hydrophila subsp. dhakensis did not produce acid from arbutin. When compared in a two-dimensional plot, the A. hydrophila subsp. dhakensis faecal isolates contained higher amounts of the two minor fatty acids C13:0 and C17:1 ω8c than the A. hydrophila subsp. hydrophila reference strain. This is the first detected occurrence of the less frequent A. hydrophila subsp. dhakensis in our region and ribotyping was proved as a suitable method for the identification of A. hydrophila subsp. dhakensis.
REFERENCES (27)
1.
Sedláček I, Jakšl V, Přepechalová H. Identification of aeromonads from water sources. Epidemiol Mikrobiol Imunol. 1994; 43: 61-66 (in Czech).
2.
Janda JM, Guthertz LS, Kokka RP, Shimada T. Aeromonas species in septicaemia: laboratory characteristics and clinical observations. Clin Infect Dis. 1994; 19: 77-83.
3.
Grobusch MP, Gobels K, Teichmann D. Cellulitis and septicaemia by Aeromonas hydrophila acquired at home. Infection. 2001; 29: 109-110.
4.
Janda JM. Recent advances in the study of the taxonomy, pathogenicity, and infectious syndromes associated with genus Aeromonas. Clin Microbiol Rev. 1991; 4: 397-410.
5.
Euzéby JP. List of Bacterial Names with Standing in Nomenclature: a folder available on the Internet. Int J Syst Bacteriol. 1997; 47: 590-592. (List of Prokaryotic names with Standing in Nomenclature. (http://www.bacterio.net, access: 2011.08.10).
6.
Martin-Carnahan A, Joseph SW. Genus I. Aeromonas. In: Garrity GM, Brenner DJ, Krieg NR, Staley JT. Bergey’s Manual of Systematic Bacteriology. Second edition. Volume Two. The Proteobacteria. Part B The Gammaproteobacteria. USA, Springer, 2005. p. 557-578.
7.
Miyake M, Iga K, Izumi C, Miyagawa A, Kobashi Y, Konishi T. Rapidly progressive pneumonia due to A. hydrophila shortly after near-drowning. Intern Med. 2000; 39: 1128-1130.
8.
Miñana-Galbis D, Urbizu-Serrano A, Farfan M, Fusté MC, Loren JG. Phylogenetic analysis and identification of Aeromonas species based on sequencing of the cpn60 universal target. Int J Syst Evol Microbiol. 2009; 59: 1976-1983.
9.
Huys G, Kämpfer P, Albert MJ, Kühn I, Denys R, Swings J. Aeromonas hydrophila subsp. dhakensis subsp. nov., isolated from children with diarrhoea in Bangladesh, and extended description of Aeromonas hydrophila subsp. hydrophila (Chester 1901) Stainer 1943. Int J Syst Evol Microbiol. 2002; 52: 705-712.
10.
Huys G, Pearson M, Kämpfer P, Denys R, Cnockaert M, Inglis V, Swings J. Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand. Int J. Syst Evol Microbiol. 2003; 53: 885-891.
11.
Abbott SL. Aeromonas. In: Murray PR, Baron EJ, Jorgenson JH, Pfaller MA, Yolken RH. Manual of Clinical Microbiology, 8th edition. Washington, D.C., ASM Press, 2003. p. 701-705.
12.
Abbott SL, Cheung WKW, Janda JM. The genus Aeromonas: biochemical characteristics, atypical reactions and phenotypic identification schemes. J Clin Microbiol. 2003; 41: 2348-2357.
13.
Abbott SL, Seli LS, Catino M Jr., Hartley MA, Janda JM. Misidentification of unusual Aeromonas species as members of genus Vibrio: a continuing problem. J Clin Microbiol. 1998; 36: 1103-1104.
14.
Andĕlová A, Porazilová I, Krejčí E. Aeromonas agar is a useful selective medium for isolating aeromonads from faecal samples. J Med Microbiol. 2006; 55: 1605-1606.
15.
Cowan ST. Cowan and Steel’s manual for the identification of medical bacteria. Cambridge, University Press, 1974. p 150-171.
16.
Páčová Z, Kocur M. New medium for detection of esterase and gelatinase activity. Zbl Bakt Hyg A. 1984; 258: 69-73.
17.
Švec P, Sedláček I, Pantůček R, Devriese LA, Doškař J. Evaluation of ribotyping for characterization and identification of Enterococcus haemoperoxidus and Enterococcus moraviensis strains. FEMS Microbiol Lett. 2001; 203: 23-27.
18.
Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994; 22: 4673-4680.
19.
Tamura K, Dudley J, Nei M, Kumar S. Phylogenetic and molecular evolutionary analyses were conducted using MEGA version 4. Mol Biol Evol. 2007; 24: 1596-1599.
20.
Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4: 406-425.
21.
Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI technical note 101. MIDI, Newark, DE, USA 2001. (http://www.midi-inc.com/media/...).
22.
MIDI, Inc. Library Generation Software user’s manual, version 4.5. MIDI, Inc., Newark, DE, USA 2002.
23.
Huys G, Vancanneyt M, Coopman R, Janssen P, Falsen E, Altwegg M, Kersters K. Cellular fatty acid composition as a chemotaxonomic marker for differentiation of phenospecies and hybridization groups in the genus Aeromonas. Int J Syst Bacteriol. 1994; 44: 651-658.
24.
Kühn I, Albert MJ, Ansaruzzaman M, Bhuiyan NA, Alabi SA, Islam MS, Neogi PK, Huys G, Janssen P, Kersters K, Möllby R. Characterization of Aeromonas spp. isolated from humans with diarrhea, from healthy controls, and from surface water in Bangladesh. J Clin Microbiol. 1997; 35: 369-373.
25.
Albert M, Faruque ASG, Faruque SM, Sack RB, Mahalanabis D. Case-control study of enteropathogens associated with childhood diarrhoea in Dhaka, Bangladesh. J Clin Microbiol. 1999; 37: 3458-3464.
26.
Farmer III JJ, Arduino MJ, Hickman-Brenner FW. The Genera Aeromonas and Plesiomonas. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K-H, Stackebrandt E. The Prokaryotes, A Handbook on the Biology of Bacteria: An Evolving Electronic Resource for the Microbiological Community. 3rd edition. Volume 6: Proteobacteria: Gamma subclass. New York, Springer-Verlag, 2006. p. 564-596. (http://link.springer-ny.com/li...).
27.
Ørmen Ø, Granum PE, Lassen J, Figueras MJ. Lack of agreement between biochemical and genetic identification of Aeromonas spp. APMIS. 2005; 113: 203-207.
Share
RELATED ARTICLE
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
We process personal data collected when visiting the website. The function of obtaining information about users and their behavior is carried out by voluntarily entered information in forms and saving cookies in end devices. Data, including cookies, are used to provide services, improve the user experience and to analyze the traffic in accordance with the Privacy policy. Data are also collected and processed by Google Analytics tool (more).
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.
You can change cookies settings in your browser. Restricted use of cookies in the browser configuration may affect some functionalities of the website.