RESEARCH PAPER
Microbiological and molecular monitoring for bovine tuberculosis in the Polish population of European bison (Bison bonasus)
1
Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, University of Life Sciences (SGGW), Warsaw, Poland
2
Department of Microbiology, National Veterinary Research Institute, Puławy, Poland
3
Department of Microbiology, National Tuberculosis Reference Laboratory, National Tuberculosis and Lung Diseases Research Institute, Warsaw, Poland
4
County Veterinary Inspectorate, Sanok, Poland
5
General Veterinary Inspectorate, Warsaw, Poland
6
Department of Animal Genetics and Conservation, Institute of Animal Sciences, University of Life Sciences (SGGW), Warsaw, Poland
Corresponding author
Anna Didkowska
Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, Poland
Department of Food Hygiene and Public Health Protection, Institute of Veterinary Medicine, University of Life Sciences (SGGW), Nowoursynowska 166, 02-787 Warsaw, Poland
Ann Agric Environ Med. 2021;28(4):575-578
KEYWORDS
TOPICS
Biological agents posing occupational risk in agriculture, forestry, food industry and wood industry and diseases caused by these agents (zoonoses, allergic and immunotoxic diseases)Prevention of occupational diseases in agriculture, forestry, food industry and wood industry
ABSTRACT
Introduction and objective:
In recent years, bovine tuberculosis (BTB) has become one of the major health hazards facing the European bison (EB, Bison bonasus), a vulnerable species that requires active protection, including regular and effective health monitoring. Monitoring of zoonotic disease in wildlife is also an important part of public health protection. The aim of the study was to determine whether BTB still influences the EB population in Poland.
Material and methods:
During 2017–2019, mandibular, retropharyngeal and mediastinal lymph nodes were collected from 90 EB during post-mortem examination, and then cultivated on Lowenstein-Jensen and Stonebrink media. Isolated strains were subjected to molecular analysis to determine the species, spoligotype and MIRU-VNTR pattern.
Results:
Lesions were found in lymph nodes originating from eight EB (8.89%). Positive microbiological cultures for mycobacteria were obtained in samples from six (6.67%) EB. The isolated strains were identified as Mycobacterium caprae (material from four EB) and atypical mycobacteria (material from two EB). For M. caprae strains spoligotype M. bovis 4_CA 1600 was identified and the MIRU-VNTR pattern was identified as 345751355413232.
Conclusions:
It is recommended that this potentially dangerous disease should be monitored in EB via a comprehensive strategy based on a combination of microbiological and molecular methods. Such monitoring will protect the health of both animals and humans
In recent years, bovine tuberculosis (BTB) has become one of the major health hazards facing the European bison (EB, Bison bonasus), a vulnerable species that requires active protection, including regular and effective health monitoring. Monitoring of zoonotic disease in wildlife is also an important part of public health protection. The aim of the study was to determine whether BTB still influences the EB population in Poland.
Material and methods:
During 2017–2019, mandibular, retropharyngeal and mediastinal lymph nodes were collected from 90 EB during post-mortem examination, and then cultivated on Lowenstein-Jensen and Stonebrink media. Isolated strains were subjected to molecular analysis to determine the species, spoligotype and MIRU-VNTR pattern.
Results:
Lesions were found in lymph nodes originating from eight EB (8.89%). Positive microbiological cultures for mycobacteria were obtained in samples from six (6.67%) EB. The isolated strains were identified as Mycobacterium caprae (material from four EB) and atypical mycobacteria (material from two EB). For M. caprae strains spoligotype M. bovis 4_CA 1600 was identified and the MIRU-VNTR pattern was identified as 345751355413232.
Conclusions:
It is recommended that this potentially dangerous disease should be monitored in EB via a comprehensive strategy based on a combination of microbiological and molecular methods. Such monitoring will protect the health of both animals and humans
ACKNOWLEDGEMENTS
The work was supported by the project “Complex project of European bison conservation by State Forests”, which is financed by the Forest Found (Poland), contract no OR.271.3.10.2017.
Didkowska A, Orłowska B, Krajewska-Wędzina M, Augustynowicz-Kopeć E, Brzezińska S, Żygowska M, Wiśniewski J, Kaczor S, Welz M, Olech W, Anusz K. Microbiological and molecular monitoring for bovine tuberculosis in the Polish population of European bison (Bison bonasus). Ann Agric Environ Med. doi: doi: 10.26444/aaem/130822
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