RESEARCH PAPER
 
KEYWORDS
TOPICS
ABSTRACT
Objective:
The main aim of this study was to determine the content of mycotoxins, such as: deoxynivalenol (DON), zearalenone (ZEA) and fumonisins (FUM) in cereal products, and such products intended for infants. The secondary objective was to assess consumer exposure to the DON, ZEA and FUM occurring in cereal products, including those intended for infants and young children.

Material and methods:
The study included cereals and cereal products such as flours, grits, pastas, products of the bakery industry, snacks and cereal products intended for infants and young children, available in retail outlets in the Małopolska Province of Poland. DON content was determined by high-performance liquid chromatography with a DAD detector, while the contents of ZEA and FUM were detected by high-performance liquid chromatography with fluorescence detection.

Results:
The determined concentration of mycotoxins exceeded the maximum level specified in food law in only two cases. DON level in maize flour was 1511.0 μg kg-1 and exceeded the maximal residue level (MRL) set at 750.0 μg kg-1. The value of MRL for ZEA was over the permissible value of 75.0 μg kg-1 in the maize flour sample only, and was 212.0 μg kg-1. None of the samples examined was beyond the permissible level of FUM.

Conclusions:
Levels higher than those permissible for the examined cereal products were noted in only two cases. FUMs were the most commonly found Fusarium mycotoxins, followed by DON and ZEA. The mean exposure doses of the assessed mycotoxins, resulting from the consumption of cereal products in the selected populations, were at low levels (reaching a maximum of 6.81%) and did not exceed the tolerable daily intake (TDI) or provisional maximum tolerable daily intake (PMTDI). Therefore, the observed average chronic exposure dose not pose a health risk to consumers.

 
REFERENCES (39)
1.
Wang L, Liao Y, Peng Z, et al. Food raw materials and food production occurrences of deoxynivalenol in different regions. Trends Food Sci Technol. 2019; 83: 41–52. https://doi.org/10.1016/j.tifs....
 
2.
Postupolski J, Starski A, Ledzion E, et al. Exposure assessment of infants and young children on selected Fusarium toxins. Ann National Instit Hygiene. 2019; 70: 5–14. https://doi.org/10.32394/rpzh.....
 
3.
Pleadin J, Vahčić N, Perši N, et al. Fusarium mycotoxins occurrence in cereals harvested from Croatian fields. Food Control. 2013; 32: 49–54. https://doi.org/10.1016/j.food....
 
4.
Stanciu O, Juan C, Berrada H, et al. Study on trichothecene and zearalenone presence in romanian wheat relative to weather conditions. Toxins. 2019; 11(3): 163–180. https://doi.org/10.3390/toxins....
 
5.
Pokrzywa P, Cieslik E, Surma M. Effect of storage conditions on the formation of type A and B trichothecenes in cereal products. Ann Agric Environ Med. 2019; 26: 260–265. https://doi.org/10.26444/aaem/....
 
6.
Chen C, Turna NS, Wu F. Risk assessment of dietary deoxynivalenol exposure in wheat products worldwide: Are new codex DON guidelines adequately protective? Trends Food Sci Technol. 2019; 89: 11–25. https://doi.org/10.1016/j.tifs....
 
7.
Simsek S, Burgess K, Whitney KL, et al. Analysis of Deoxynivalenol and Deoxynivalenol-3-glucoside in wheat. Food Control. 2012; 26: 287–292. https://doi.org/10.1016/j.food....
 
8.
Latorre A, Dagnac T, Lorenzo BF, et al. Occurrence and stability of masked fumonisins in corn silage samples. Food Chem. 2015; 189: 38–44. https://doi.org/10.1016/j.food....
 
9.
Reisinger NN, Dohnal I, Nagl V, et al. Fumonisin B1 (FB1) induces lamellar separation and alters sphingolipid metabolism of in vitro cultured hoof explants. Toxins. 2016; 8(4): 89. https://doi.org/10.3390/toxins....
 
10.
Kowalska K, Habrowska-Górczynska DE, Piastowska-Ciesielska AW. Zearalenone as an endocrine disruptor in humans. Environ Toxicol Pharmacol. 2016; 48: 141–149. https://doi.org/10.1016/j.etap....
 
11.
Commission Regulation (EC) No 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. L.2006; 364: 5–24.
 
12.
EFSA (European Food Safety Authority) Evaluation of the increase of risk for public health related to a possible temporary derogation from the maximum level of deoxynivalenol, zearalenone and fumonisins for maize and maize products, EFSA J. 2014; 12(5): 3699. https://doi.org/10.2903/j.efsa....
 
13.
Commission Regulation (EC) No 401/2006 of 23 February 2006 laying down the methods of sampling and analysis for the official control of the levels of mycotoxins in foodstuffs. L. 2006; 70: 12–34, 2006.
 
14.
EFSA (European Food Safety Authority), 2010. Management of left-censored data in dietary exposure assessment of chemical substances. EFSA J. 2010; 8: 1557. https://doi.org/10.2903/j.efsa....
 
15.
Vidal A, Morales H, Sanchis V, et al. Stability of DON and OTA during the breadmaking process and determination of process and performance criteria. Food Control. 2014; 40: 234–242. https://doi.org/10.1016/j.food....
 
16.
Jarosz M, Rychlik E, Stoś K, et al. Nutritional norms for the Polish population and their application. https://www.pzh.gov.pl/wp-cont... (access: 2021.06.20).
 
17.
Dobosz B, Król K, Lar K, et al. Presence of mycotoxins in cereal preparations on the market in Silesia Province from 2013 to 2015. Environ Med. 2017; 20(1): 34–40. https://doi.org/10.19243/20171....
 
18.
Mruczyk K, Mizgier M, Wójciak RW, et al. Comparison of deoxynivalenol and zearaleone concentration in conventional and organic cereal products in western Poland. Ann Agric Environ Med. 2021; 28(1): 44–48. https://doi.org/10.26444/aaem/....
 
19.
Darsanaki RK, Issazadeh K, Aliabadi MA, et al. Occurrence of Deoxynivalenol (DON) in wheat flours in Guilan Province, northern Iran. Ann Agric Environ Med. 2015; 22(1): 35–37. https://doi.org/10.5604/123219...
 
20.
Malachova A, Dzuman Z, Veprikova Z, et al. Deoxynivalenol, deoxynivalenol-3-glucoside, and enniatins: The major mycotoxins found in cereal-based products on the Czech market. J Agric Food Chem. 2011; 59: 12990–12997. https://doi.org/10.1021/jf2033....
 
21.
González-Osnaya L, Cortés C, Soriano J, et al. Occurrence of deoxynivalenol and T-2 toxin in bread and pasta commercialised in Spain. Food Chemistry. 2011; 124: 156–161. https://doi.org/10.1016/j.food....
 
22.
Golge O, Kabak B. Occurrence of deoxynivalenol and zearalenone in cereals and cereal products from Turkey. Food Control. 2020; 110: 106982, https://doi.org/10.1016/j.food....
 
23.
Sirot V, Fremy JM, Leblanc JC. Dietary exposure to mycotoxins and health risk assessment in the second French total diet study. Food Chem Toxicol. 2012; 52: 1–11. https://doi.org/10.1016/j.fct.....
 
24.
Cano-Sancho G, Marin S, Ramos AJ, et al. Occurrence of zearalenone, an oestrogenic mycotoxin, in Catalonia (Spain) and exposure assessment. Food Chem Toxicol. 2012; 50: 835–839. https://doi.org/10.1016/j.fct.....
 
25.
Ostry V, Dofkova M, Blahova J, et al. Dietary exposure assessment of sum deoxynivalenol forms, sum T-2/HT-2 toxins and zearalenone from cereal-based foods and beer. Food Chem Toxicol. 2020; 139: 111280. doi: 10.1016/j.fct.2020.111280.
 
26.
Juan C, Raiola A, Manes J, et al. Presence of mycotoxin in commercial infant formulas and baby foods from Italian market. Food Control. 2014;39:227–236. https://doi.org/10.1016/j.food....
 
27.
Kowalska A, Hajok I, Piekut A. Assessment of the contamination level by Fumonisins B1 and B2 of the corn food products available on Polish consumer market. Pol J Environ Stud. 2017; 26(6): 2595–2601. https://doi.org/10.15244/pjoes....
 
28.
Martins FA, Ferreira FMD, Ferreira FD, et al. Daily intake estimates of fumonisins in corn-based food products in the population of Parana, Brazil. Food Control. 2012; 26: 614–618. https://doi.org/10.1016/j.food....
 
29.
Cano-Sancho G, Ramos AJ, Marin S, et al. Occurrence of fumonisins in Catalonia (Spain) and an exposure assessment of specific population groups. Food Addit. Contam Part A. 2012; 29: 799–808. https://doi.org/10.1080/194400....
 
30.
Galbenu P, Damiescu L, Trif A. Fumonisin occurrence in cereal and cereal-based foodstuffs marketed in Timis County. Res J Agric Sci. 2011; 43(1): 50.
 
31.
Stanciu O, Juan C, Miere D, et al. First study on trichothecene and zearalenone exposure of the Romanian population through wheatbased products consumption. Food Chem Toxicol. 2018; 121: 336–342. https://doi.org/10.1016/j.fct.....
 
32.
Pleadin J, Staver MM, Markov K, et al. Mycotoxins in organic and conventional cereals and cereal products grown and marketed in Croatia. Mycotoxin Res. 2017; 33: 219–227. https://doi.org/10.1007/s12550....
 
33.
Rodríguez-Carrasco Y, Moltó JC, Berrada H, et al. A survey of trichothecenes, zearalenone and patulin in milled grain-based products using GC-MS/MS. Food Chem. 2014; 146: 212–219. https://doi.org/10.1016/j.food....
 
34.
Assunçao R, Martins C, Vasco E, et al. Portuguese children dietary exposure to multiple mycotoxins – an overview of risk assessment under MYCOMIX project. Food Chem Toxicol. 2018; 118: 399–408. https://doi.org/10.1016/j.fct.....
 
35.
Fleury S, Riviere G, Alles B, et al. Exposure to contaminants and nutritional intakes in a French vegetarian population. Food Chem Toxicol. 2017; 109: 218–229. https://doi.org/10.1016/j.fct.....
 
36.
Do TH, Tran SC, Le CD, et al. Dietary exposure and health risk characterization of aflatoxin B1, ochratoxin A, fumonisin B1, and zearalenone in food from different provinces in Northern Vietnam. Food Control. 2020; 112: 107–108. https://doi.org/10.1016/j.food....
 
37.
Huong BTM, Brimer L, Dalsgaard A. Dietary exposure to aflatoxin B1, ochratoxin A and fuminisins of adults in Lao Cai province, Vietnam: A total dietary study approach. Food Chem Toxicol. 2016; 98: 127–133. https://doi.org/10.1016/j.fct.....
 
38.
Kirimker SE, Turksoy S, Kabak B. Assessment of dietary exposure to deoxynivalenol and fumonisin in the population of infants and toddlers in Turkey. Food Chem Toxicol. 2020; 140: 111304. https://doi.org/10.1016/j.fct.....
 
39.
EFSA (European Food Safety Authority), 2014. Scientific opinion on the risks for human and animal health related to the presence of modified forms of certain mycotoxins in food and feed. EFSA J. 2014; 12: 3916. https://doi.org/10.2903/j.efsa....
 
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