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RESEARCH PAPER
 
CC BY-NC-ND 3.0
 
 

Ethanol influence on gingival fibroblasts – a real-time in vitro study

 
1
Department of Conservative Dentistry and Periodontology, University of Medical Sciences, Poznan, Poland
2
University of Life Sciences, Poznan, Poland
3
University of Medical Sciences, Poznan, Poland
4
Medical University, Wroclaw, Poland
KEYWORDS:
TOPICS:
ABSTRACT:
Introduction:
Alcohol consumption is the world’s third largest risk factor for disease and disability. According to the WHO report from 2011: 71% of urban respondents ty and 77% of rural respondents admit to alcohol consumption]. Lower socio-economic status and educational levels result in a greater risk of alcohol-related injury, disease and death. Alcohol is a common component of many medicines, as well as an ingredient in many oral hygiene home products. Mouthwashes containing alcohol are considered to inhibit wound healing in the oral cavity. Due to the fact that many different results are described for different concentrations of alcohol at different times, an attemptwas made to visualise the direct impact of 7.2% and 22% alcohol on human gingival fibroblasts.

Material and methods:
PANsystem 2000 was used for visualisation of the reaction of human gingival fibroblasts isolated from gingiva on ethanol in 2 different concentrations. PANsys 3000 is a multi-system fully-automated cell culture device used for in vitro culture and to study a variety of cell lines under conditions similar to in vivo. Observations were carried out for 48 hours since alcohol addition. Pictures were taken in a continuous process at 5 minute intervalds and combined into a film.

Results:
Both contamination of 7.2% and 22% ethyl alcohol negatively affected morphology and cell proliferation. Addition of ethanol at a concentration 7.2% enabled cells to regain their ability to divide and recover normal morphology after 10 hours; changes caused by 22% ethanol, however, were irreversible.

Conclusions:
The obtained results suggest that daily usage of 7.2% alcohol contained in mouthwashes is non-toxic for gingival fibroblasts, and could be recommended after periodontal surgery.

CORRESPONDING AUTHOR:
Marzena Wyganowska-Świątkowska   
Department of Conservative Dentistry and Periodontology , Poznan University of Medical Sciences, Bukowska 70, 60-812 Poznań, Poland
 
REFERENCES (32):
1. Petersen PE. Strengthening the prevention of oral cancer: the WHO perspective. Community Dentistry Oral Epidemiology 2005; 33: 397–399.
2. Global status report on alcohol and health. WHO Press Geneva, 2011.
3. Moritani K, Takeshita T, Shibata Y, Ninomiya T, Kiyohara Y, Yamashita Y. Acetaldehyde production by major oral microbes. Oral Dis. 2015; 21: 748–754.
4. McCullough MJ, Farah CS. The role of alcohol in oral carcinogenesis with particular reference to alcohol-containing mouthwashes. Australian Dent J. 2008; 53: 302–305.
5. Carlin V, Matsumoto MA, Saraiva PP, Artioli A, Oshima CT, Ribeiro DA. Cytogenetic damage induced by mouthrinses formulations in vivo and in vitro. Clin Oral Invest. 2012; 16: 813–820.
6. San Miguel SM, Opperman LA, Allen EP, Zielinski J, Kathy KH. Bioactive polyphenol antioxidants protect oral fibroblasts from ROS-inducing agents. Arch Oral Med. 2012; 57: 1657–1667.
7. Chevalier M, Sakarovitch C, Precheur I, Lamure J, Pouyssegur-Rougier V. Antiseptic mouthwashes could worsen xerostomia in patients taking polypharmacy. Acta Odontol Scand. 2015; 73: 267–273.
8. Gagari E, Kabani S. Adverse effects of mouthwash use. Oral Surgery Oral Medicine Oral Pathology Oral Radiology Endodontics. 1995; 80: 432–439.
9. Shulman JD, Well LM. Acute ethanol toxicity from ingesting mouthwash in children younger than 6-years of age. Pediatric Dentistry. 1997; 19: 404–408.
10. Addy M. Oral hygiene products: potential for harm to oral and systemic health?. Periodontology 2000. 2008; 48: 54–65.
11. Stephens P, al-Khateeb T, Davies KJ, Shepherd JP, Thomas DW. An investigation of the interaction between alcohol and fibroblasts in wound healing. Int J Oral Maxillofacial Surg. 1996; 25: 161–164.
12. Szabo J, Bikash VK, Fogarasi M, Cata-Lano DE. Induction of transforming growth factor-beta and prostaglandin E2 production by monocytes. J Leukocyte Biol. 1992; 52: 602–610.
13. Simanowski UA, Stickel F, Maier H, Gartner U, Seitz HK. Effect of alcohol on gastrointestinal cell regeneration as a possible mechanism in alcohol-associated carcinogenesis. Alcohol, 1995; 12: 111–115.
14. Aroor A, Baker RC. Negative and positive regulation of astrocyte DNA synthesis by ethanol. J Neurosci Res. 1997; 50: 1010–1017.
15. Wyganowska-Swiatkowska M, Urbaniak P, Szkaradkiewicz A, Jankun J, Kotwicka M. Effects of chlorhexidine, essential oils and herbal medicines (Salvia, Chamomile, Calendule) on human fibroblast in vitro. Centr Eur J Immunol. 2016; 41: 1–7.
16. Boisnic S, Ben Slama L, Branchet-Gumila MC, Watts M, D’Arros G.Wound healing effect of Eludril in a model of human gingival mucosa. Revue Stomatologie Chirurgie Maxillo-faciale. 2006; 107: 431–435.
17. Luo J, Miller MW. Ethanol inhibits basic fibroblast growth factor-mediated proliferation of C6 astrocytoma cells. J Neurochem. 1996; 67:1448–1456.
18. Luo J, Miller MW. Growth factor-mediated neural proliferation: Target of ethanol toxicity. Brain Res Rev. 1998; 27: 157–167.
19. Luo J, West JR, Pantazis NJ. Nerve growth factor and basic fibroblast growth factor protect rat cerebellar granule cells in culture against ethanol-induced cell death. Alcohol Clin Experimental Res. 1997; 21: 1108–1120.
20. Cartwright MM, Tessmer LL, Smith SM. Ethanol-in- duced neural crest apoptosis is coincident with their endog- enous death, but is mechanistically distinct. Clin Experimental Res. 1998; 22:142–149.
21. Shepherd S. Alcohol consumption a risk factor for periodontal disease. Evidence-Based Dentistry. 2011; 12: 76.
22. Hendriks T, Martens MFWC, Huyben CMLC, Wobbes T. Inhibition of basal and TGF-B induced fibroblast collagen synthesis by antineoplastic agents. Implications for wound healing. British J Cancer. 1993; 67: 545–50.
23. Irie K, Tomofuji T, Tamaki N, Sanbe T, Ekuni D, Azuma T, et al. Effects of ethanol consumption on periodontal inflammation in rats. J Dental Res. 2008; 87: 456–60.
24. Amaral Cda S, Vettore MV, Lea ̃o A. The relationship of alcohol dependence and alcohol consumption with periodontitis: a systematic review. J Dentistry. 2009; 37: 643–651.
25. Ranzer MJ. Fibroblast function and wound breaking strength is impaired by acute ethanol intoxication. Alcohol Clin Exper Res. 20011; 35: 83–90.
26. Guha N, Boffetta P, Wünsch V, Eluf Neto J, Shangina O, Zaridze D, et al. Oral health and risk of squamous cell carcinoma of the head and neck and esophagus: results of two multicentric case-control studies. Am J Epidemiol. 2007; 166: 1159–1173.
27. Howie NM, Trigkas TK, Cruchley AT, Wertz PW, Squier CA, Williams DM. Short term exposure to alcohol increases the permeability of human oral mucosa. Oral Dis. 2001; 7: 340–354.
28. Bagan JV, Vera-Sempere F, Marzal C, Pellín-Carcelén A, Martí-Bonmatí E, Bagan L. Cytological changes in the oral mucosa after use of a mouth rinse with alcohol: A prospective double blind control study. Medicina Oral Patologia Oral Cirurgia Bucal. 2012; 17: e956–961.
29. Huang JS, She QB, Crilly KS, Kiss Z. Ethanol, Zn2+ and insulin interact asprogression factors to enhance DNA synthesis synergistically in the presence of Ca2+ and other cell cycle initiators in fibroblasts. Biochem J. 2000; 346: 241–247.
30. Li C, Peoples R, Weight FF. Alcohol action on a neuronal membrane receptor: Evidence for a direct interaction with the receptor protein. Proceedings National Academy Science USA. 1994; 19: 8200 -8204.
31. Channareddy S, Nguyen NT, Janes N. Saturable ethanol binding in rat liver mitochondria. Biochimica Biophysica Acta. 2000; 1463: 291–300.
32. Crilly KS, Benyhe S, Kiss Z. Promitogenic effects of ethanol, methanol, and ethanolamine in insulin-treated fibroblasts. Biochem Pharmacol. 2000; 60: 1391–1398.
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