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RESEARCH PAPER
 
CC BY-NC-ND 3.0
 
 

Detection and identification of six Cryptospordium species in livestock in Slovakia by amplification of SSU and GP60 genes with the use of PCR analysis

Oľga Danišová 1  ,  
 
1
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
Ann Agric Environ Med 2016;23(2):254–258
KEYWORDS:
ABSTRACT:
Introduction:
In this study we examined 200 faecal samples from pigs and calves with suspected cryptosporidiosis were examined by the PCR methods: nested PCR for amplification of SSU region; nested PCR for amplification of GP60 region; and with restriction analysis of DNA (PCR-RFLP). The sequencing identified the following species: Cryptosporidium muris (2), Cryptosporidium andersoni (1), Cryptosporidium bovis (4), Cryptosporidium suis (2), Cryptosporidium scrofarum (10), mixed infection caused by C. scrofarum and C. muris (1),and Cryptosporidium parvum (10) genotype A subtype IIaA17G2R1.

Results and conclusions:
The findings suggest that livestock can be an important source of zoonotic species or genotypes of Cryptosporidium, which may adversely affect the public health of human populations. This is the first time in our country that the Cryptosporidium species has been identified in livestock in Slovakia. The identification and genotyping of this pathogen in Slovakia, completes the epidemiological situation in Europe for Cryptosporidum species.

CORRESPONDING AUTHOR:
Oľga Danišová   
Department of Biology and Genetics, University of Veterinary Medicine and Pharmacy, Košice, Slovak Republic
 
REFERENCES:
1. Mcanulty JM, Fleming DW, Gonzales AM. A community-wide outbreak of cryptosporidiosis associated with swimming at a wave pool. J Am Med Associat. 1994; 272: 1597–1600.
2. Xiao L, Moore JE, Ukoh U, Gatei W, Lowery CJ, Murphy TM, Dooley JS, Millar BC, Rooney PJ, Rao JR. Prevalence and identity of Cryptosporidium spp. in pig slurry. Appl Environ Microbiol. 2006; 72: 4461–4463.
3. Chen F, Huang K.Prevalence and phylogenetic analysis of Cryptosporidium in pigs in eastern China. Zoonoses Public Health. 2007; 54: 393–400.
4. Hůrková LA, Modrý D.Cryptosporidium muris – původce žaludeční kryptosporidiózy hlodavců. Veterinářství. 2003; 53: 230–232.
5. Sanford SE. Enteric cryptosporidial infection in pigs: 184 cases (1981- 1985). J Am Vet Med Assoc. 1987; 190: 695–698.
6. Enemark HL, Ahrens P, Bille-Hansen V, Heegaard PMH, Vigre H, Thamsborg SM, Lind P.Cryptosporidium parvum infectivity and pathogenicity of the ‘porcine’ genotype. Parasitol. 2003; 126: 407–416.
7. Núñez A, Mcneilly F, Perea A, Sánchez-Cordón PJ, Huerta B, Allan G, Carrasco L. Coinfection by Cryptosporidium parvum and porcine circovirus type 2 in weaned pigs. J Vet Med B Infect Dis Vet Public Health. 2003; 50: 255–258.
8. Rommel M, Eckert J, Kutzer E, Boch J, Supperer R. Veterinärmedizinische. Parasitologie Parey, 2000; 431–436.
9. Castro-Hermida JA, Almeida A, Gonzales-Warleta M, Corrlia De Costa JM, Rumbo-Lorenzo C, Mezo M. Occurrence of Cryptosporidium parvum and Giardia duodenalis in healthy adult domestic ruminants. Parasitol Res. 2007; 101(5): 1443–1448.
10. Feng Y, Ortega Y, He G, Das P, Xu M, Zhang X, Fayer R, Gatei W, Cama V, Xiao L. Wide geographic distribution of Cryptosporidium parvum and deer like genotype in bovines. Vet Parasitol. 2007; 144: 1–9.
11. Xiao L, Ryan UM.Cryptosporidiosis: an update in molecular epidemiology. Curr Opin Infect Dis. 2004b; 17: 483–90.
12. Ma J, Cai J, Ma J, Feng Y, Xiao L. Occurrence and molecular characterization of Cryptosporidium spp. in yaks (Bos grunniens) in China. Vet Parasitol. 2014; 202(3–4): 113–118.
13. Xiao L, Morgan UM, Limor J, Escalante A, Arrowood M, Shulaw W, Thompson RC, Fayer R, Lal AA. Genetic diversity within Cryptosporidium parvum andrelated Cryptosporidium species. Appl Environ Microbiol. 1999; 65: 3386–3391.
14. Leetz AS, Sotiriadou I, Ongerth J, Karanis P. An evaluation of primers amplifying DNA targets for the detection of Cryptosporidium spp. using C. parvum HNJ-1 Japanese isolate in water samples. Parasitol Res. 2007; Sep;101(4): 951–962.
15. Xiao L, Bern C, Limor J, Sulaiman I, Roberts J, Checkley W, Cabrera L, Gilman RH, Lal AA. Identification of 5 types of Cryptosporidium parasites in children in Lima, Peru. J Infect Dis. 2001; 183: 492– 97.
16. Stamatakis A, Hoover P, Rougemont J. Systematic Biology. 2008; 75(5): 758–771.
17. Maddox-Hyttel C, Langkjaer RB, Enemark HL, Vigre H.Cryptosporidium and Giardia in different age groups of Danish cattle and pigs occurrence and management associated risk factors. Vet Parasitol. 2006; 141: 48–59.
18. Suárez-Luengas L, Clavel A, Quílez J, Goñi-Cepero MP, Torres E, Sánchez-Acedo C, Del Cacho E.Molecular characterization of Cryptosporidium isolates from pigs in Zaragoza (northeastern Spain). Vet Parasitol. 2007; 148: 231–235.
19. Xiao L, Herd RP, McClure KE. Periparturient rise in the excretion of Giardia cysts and Cryptosporidium parvum oocysts as a source of infection for lambs. J Parasitol. 1994; 80: 55–59.
20. Atwill ER, Sweitzer RA, Pereira MG, Gardner IA, Van Vuren D, Boyce WM. Prevalence of and associated risk factors for shedding Cryptosporidium parvum oocysts and Giardia cysts within feral pig populations in California. Appl Environ Microbiol. 1997; 63: 3946–3949.
21. Quílez J, Sánchez-Acedo C, Clavel A, Del Cacho E, López-Bernad F. Prevalence of Cryptosporidium infections in pigs in Aragón (northeastern Spain). Vet Parasitol.1996; 67: 83–88.
22. Vítovec J, Hamadejová K, Landová L, Kváč M, Květoňová D, Sak B. Prevalence and pathogenicity of Cryptosporidium suis in pre- and post-weaned pigs. J Vet Med B. 2006; 53: 239–243.
23. Kváč M, Sak B, Hanzlíková D, Kotilová J, Kvetonová D. Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic. Parasitol Res. 2009a; 104(2): 425–428.
24. Jenkins MB, Liotta JL, Lucio-Forster A, Bowman DD. Concentrations, viability, and distrribution of Cryptosporidium genotypes in lagoons ofswine facilities in the Southern Piedmont and in coastal pain watersheds of Georgia. Appl Enviroment Microbiol. 2010 Sep;76(17): 5757–63.
25. Kváč M, Kestřánová M, Květoňová D, Kotková M, Ortega Y, McEvoy J, Sak B. Cryptosporidium tyzzeri and Cryptosporidium muris originated from wild West-European house mice (Mus musculus domesticus) and East-European house mice (Mus musculus musculus) are non-infectious for pigs. Exp Parasitol. 2012; May; 131(1): 107–10.
26. Hasajová A, Valenčáková A, Malčeková B, Danišová O, Halán M, Goldová M, Sak B, Květoňová D, Kváč M, Halánová M. Significantly highter occurrence of Cryptosporidium infection in Roma children compared with non. Roma children in Slovakia. Eur J Clin Microbiol Infect Dis. 2014 Aug; 33(8): 1401–1406.
27. Kváč M, Hanzlíková D, Sak B, Kvetonová D. Prevalence and age-related infection of Cryptosporidium suis, C. muris and Cryptosporidium pig genotype II in pigs on a farm complex in the Czech Republic. Vet Parasitol. 2009; Mar 23; 160(3–4): 319–322.
28. Kváč M, Kvetonová D, Sak B, Ditrich O. Cryptosporidium pig genotype II in immunocompetent man. Emerg Infect Dis. 2009b Jun; 15(6): 982–983.
29. Santín M, Trout J, Xiao L, Zhou L, Greiner E, Fayer R. Prevalenceand age related variation of Cryptosporidium species and genotypes indairy calves. Vet Parasitol. 2004; 122: 103–117.
30. Rieux A, Paraud C, Pors I, Chartier C. Molecular characterization of Cryptosporidium isolates from beef calves under one month of age over three successive years in one herd in western France.Vet Parasitol. 2014May; 28; 202(3–4): 171–179.
31. Bednarska M, Bajer A, Siński E. Calves as a potential reservoir of Cryptosporidium parvum and Giardia sp. Ann Agric Environ Med. 1998; 5(2): 135–8.
32. Santín M, Trout JM, Fayer R. A longitudinal study of cryptospo-ridiosis in dairy cattle from birth to 2 years of age. Vet Parasitol. 2008; 155: 15–23.
33. Coklin T, Uehlinger FD, Farber JM, Barkema HW, O’Handley RM, Dixon BR. Prevalence and molecular characterization of Cryptosporidium spp. in dairy calves from 11 farms in Prince Edward Island, Canada. Vet Parasitol. 2009; 160: 323–326.
34. Kváč M, Kouba M, Vítovec J. Age-related and housing-dependence of Cryptosporidium infection of calves from dairy and beefherds in South Bohemia, Czech Republic. Vet Parasitol. 2006; 137: 202–209.
35. Fayer R, Santín M, Trout JM. Cryptosporidium ryanae n. sp. (Api-complexa: Cryptosporidiidae) in cattle (Bos taurus). Vet Parasitol. 2008; 156: 191–198.
36. Plutzer J, Karanis P. Genotype and subtype analyses of Cryptosporidium isolates from cattle in Hungary. Vet Parasitol. 2007; 146: 357–362.
37. Silverlås C, Bosaeus-Reineck H, Näslund K, Björkman C. Is therea need for improved Cryptosporidium diagnostics in Swedish calves? Int J Parasitol. 2013; 43: 155–161.
38. Alves M, Xiao L, Antunes F, Matos O. Distribution of Cryptosporidium subtypes in humans and domestic and wild ruminants in Portugal. Parasitol Res. 2006; 99: 287–292.
39. Soba B, Logar J. Genetic classification of Cryptosporidium isolatesfrom humans and calves in Slovenia. Parasitol. 2008; 135: 1236–1270.
40. Wielinga PR, de Vries A, Van der Goot TH, Mank T, Mars MH, Kort-beek LM, van der Giessen JW. Molecular epidemiology ofCryptosporidium in human and cattle in the Netherlands. Int J Parasitol. 2008; 38: 809–817.
41. Follet J, Guyot K, Leruste H, Follet-Dumoulin A, Hammouma-Ghelboun O, Certad G, Dei-Cas E, Halama P.Cryptosporidium infection in a veal calf cohort in France: molecular characterization of species in a longitudinal study. Vet Res Dec. 2011; 2; 42:116.
42. Rieux A, Paraud C, Pors I, Chartier C. Molecular characterizationof Cryptosporidium isolates from pre-weaned calves in western Francein relation to age. Vet Parasitol. 2013b; 197: 7–12.
43. Thompson HP, Dooley JS, Kenney J, Mccoy M, Lowery JE, Moore JE, Xiao L. Genotypes and subtypes of Cryptosporidium spp. in neonatal calves in Northern Ireland. Parasitol Res. 2007; 100: 619–624.
44. Broglia A, Reckinger S, Cacció SM, Nöckler K. Distribution of Cryptosporidium parvum subtype in calves in Germany. Vet Parasitol. 2008; 14; 154(1–2): 8–13.
45. Duranti A, Cocció SM, Pozio E, Di Egidio A, De Curtis M, Battisti A, Scaramozzino P. Risk factors associated with Cryptosporidium parvum infection in cattle. Zoonoses Public Health. 2009; 56(4): 176–182.
46. Quílez J, Sánchez-Acedo C, Clavel A, Del Cacho E, López-Bernad F. Prevalence of Cryptosporidium infections in pigs in Aragón (northeastern Spain). Vet Parasitol. 1999; 67:83–88.
47. Trotz-Williams LA, Martin DS, Gatei W, Cama V, Peregrine AS, Martin SW, Nydam DV, Jamieson F, Xiao L. Genotype and subtype analyses of isolates from dairy calves and humans in Ontario. Parasitol Res. 2006; 99: 346–352.
48. Xiao L, Zhou L, Santin M, Yang W, Fayer R. Distribution of Cryptosporidium parvum subtypes in calves in eastern United States. Parasitol Res. 2007; 100: 701–706.
49. Mi R, Wang X, Huang Y, Zhou P, Liu Y, Chen Y, Chen J, Zhu W, Chen Z. Prevalence and molecular characterization of Cryptosporidium in Goats across four provincial level area in China. PLOS One. 2014; 24; 9(10): e111164.
50. Center for Disease Control and Prevention (CDC).Cryptosporidium Outbreak at summer camp – North Carolina. MMWR Mord Mortal Wkly Rep. 2011; 60(27): 918–922.
51. Koehler AV, Brandbury RS, Stevens MA, Haydon SR, Jex AR, Gasser RB. Genetic characterization of selected parasites from people with histories of gastrointestinal disorders using a mutation scanning-coupled approach. Electrophoresis. 2011; 34(112): 1720–1728.
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