RESEARCH PAPER
Neospora caninum and Toxoplasma gondii antibodies in red foxes (Vulpes vulpes) in the Czech Republic
 
More details
Hide details
1
University of Veterinary and Pharmaceutical Sciences, Faculty of Veterinary Hygiene and Ecology, Department of Biology and Wildlife Diseases, Brno, Czech Republic
2
State Veterinary Institute, Prague, Czech Republic
CORRESPONDING AUTHOR
Eva Bártová   

University of Veterinary and Pharmaceutical Sciences, Faculty of Veterinary Hygiene and Ecology, Department of Biology and Wildlife Diseases, Brno, Czech Republic
 
Ann Agric Environ Med. 2016;23(1):84–86
KEYWORDS
ABSTRACT
Introduction:
Neospora caninum and Toxoplasma gondii are worldwide spread parasites, causing serious illnesses in sensitive animals; toxoplasmosis is also important zoonosis. Although neosporosis is not considered as a zoonosis, it leads to aborted births in cattle, as well as paresis and paralysis in dogs.

Objective:
The aim of this study was to discover the prevalence of N. caninum and T. gondii antibodies in red foxes (Vulpes vulpes) in the Czech Republic.

Material and Methods:
Sera of 80 foxes from 8 regions of the Czech Republic were tested for antibodies to N. caninum and T. gondii by competitive enzyme linked immunosorbent assay (cELISA) and indirect ELISA. All samples were simultaneously tested by indirect fluorescent antibody test (IFAT) to detect both N. caninum and T. gondii antibodies.

Results:
Antibodies to N. caninum were found by IFAT in 3 (3.8%) red foxes with titre 50 and in 2 (2.5%) red foxes with inhibition 42.7% and 30.2 %. Antibodies to T. gondii were found in all tested animals in both IFAT (titres 50 – 6400) and in ELISA (S/P ranging from 34% – 133%).

 
REFERENCES (15)
1.
Dubey JP, Schares G. Neosporosis in animals – the last five years. Vet Parasitol. 2011; 180: 90–108.
 
2.
Dubey JP (Ed.). Toxoplasmosis of animals and humans, 2nd ed. CRC Press, Taylor and Francis Group, Boca Raton, Florida, 2010.p.5–221.
 
3.
Schares G, Heydorn AO, Cuppers A, Mehlhorn H, Geue L, Peters M, Conraths FJ. In contrast to dogs, red foxes (Vulpes vulpes) did not shed Neospora caninum upon feeding of intermediate host tissues. Parasitol Res. 2002; 88: 44–52.
 
4.
Constantin EM, Schares G, Grossmann E, Sauter K, Romig T, Hartmann S. Studies of the role of the red fox (Vulpes vulpes) as a potential definitive host of Neospora caninum. Berl Munch Tier Woch. 2012; 124: 148–153.
 
5.
eAGRI. Výkaz myslivosti. IOP Publishing eAGRI. http://eagri.cz/public/web/fil... (access: 2013.07.23).
 
6.
Sobrino R, Dubey JP, Pabon M, Linarez N, Kwok OC, Millan J, et al. Neospora caninum antibodies in wild carnivores from Spain. Vet Parasitol. 2008; 155: 190–197.
 
7.
Buchton D, Maley SW, Pastoret PP, Brochier B, Innes EA. Examination of red foxes (Vulpes vulpes) from Belgium for antibody to Neospora caninum and Toxoplasma gondii. Vet Rec. 1997; 141: 308–309.
 
8.
Herrmann DC, Maksimov P, Maksimov A, Sutor A, Schwarz S, Jaschke W, et al. Toxoplasma gondii in foxes and rodents from the German Federal States of Brandenburg and Saxony-Anhalt: Seroprevalence and genotypes. Vet Parasitol. 2012; 185: 78–85.
 
9.
Verin R, Mugnaini L, Nardoni S, Papini RA, Ariti G, Poli A, et al. Serologic, molecular, and pathologic survey of Toxoplasma gondii infection in free-ranging red foxes (Vulpes vulpes) in central Italy. J Wild Dis. 2013; 49: 545–551.
 
10.
Jakubek EB, Farkas R, Palfi V, Mattsson JG. Prevalence of antibodies against Toxoplasma gondii and Neospora caninum in Hungarian red foxes (Vulpes vulpes). Vet Parasitol. 2007; 144: 39–44.
 
11.
Jakubek EB, Brojer C, Regnersen C, Uggla A, Schares G, Bjorkman C. Seroprevalences of Toxoplasma gondii and Neospora caninum in Swedish red foxes (Vulpes vulpes). Vet Parasitol. 2001; 102: 167–172.
 
12.
Wanha K, Edelhofer R, Gabler-Eduardo C, Prosl H. Prevalence of antibodies against Neospora caninum and Toxoplasma gondii in dogs and foxes in Austria. Vet Parasitol. 2005; 128: 189–193.
 
13.
Hurkova L, Modry D. PCR detection of Neospora caninum, Toxoplasma gondii and Encephalitozoon cuniculi in brains of wild carnivores. Vet Parasitol. 2006; 137: 150–154.
 
14.
Kidawa D, Kowalczyk R. The effects of sex, age, season and habitat on diet of the red fox Vulpes vulpes in northeastern Poland. Acta Theriol. 2011; 56: 209–218.
 
15.
Bártová E, Sedlák K, Treml F, Holko I, Literak I. Neospora caninum and Toxoplasma gondii antibodies in European brown hares in the Czech Republic, Slovakia and Austria. Vet Parasitol. 2010; 171: 155–158.
 
eISSN:1898-2263
ISSN:1232-1966