RESEARCH PAPER
Brucella suis biovar 2 isolations from cattle in Poland
 
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Department of Microbiology, National Veterinary Research Institute, Pulawy, Poland
 
Ann Agric Environ Med. 2013;20(4):672–675
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ABSTRACT
Bovine brucellosis is an infectious disease caused by bacteria of the Brucella genus, primarily by B. abortus, less frequently by B. melitensis, and occasionally by B. suis. In the European Union, brucellosis in cattle has been eradicated in most of the Member States, which are recognized as ‘officially free from bovine brucellosis’. Nevertheless, cattle herds continue to be serologically monitored for the potential re-emergence of the disease. The aim of the presented study was to show the results of bacteriological investigations of cattle slaughtered in Poland in years 2002–2011 on account of positive serological reactions for brucellosis. Specimens (sera and tissues) from 176 cows were examined. Sera from the animals were tested using RBT(rose bengal test), SAT (serum agglutination test), CFT (complement fixation test), 2-ME (2-mercaptoethanol test), Coombs (Coombs antiglobulin test) and ELISA (enzyme linked immunosorbant assay). Tissue samples were cultured for Brucella, according to official protocols. All sera were RBT and SAT-positive, 170 of them were CFT- positive, whereas 6 other samples were CFT negative while positive in Coombs and ELISA. In bacteriological examination, B. abortus was not isolated. On the other hand, B. suis biovar 2 was isolated from 5 cows, which had never been reported previously in Poland. Three cows came from the same herd. Conventional, as well as, molecular investigations based on PCR methods, confirmed that the bacteria isolated bacteria belong to the B. suis biovar 2. In Poland, as in many other European countries, wildlife (wild boars and hares) constitutes a huge reservoir of the said biovar. The results of the presented research indicate that B. suis biovar 2 can easily infect cattle, and undoubtedly plays a role in the epidemiology and control of bovine brucellosis.
 
REFERENCES (37)
1.
Council Directive 64/432/EEC of 26 June 1964 on animal health problems affecting intra Community trade in bovine animals and swine, 1964.
 
2.
Commission Decision of 5 August 2009 amending Decision 2003/467/EC as regards the declaration that certain Member States and regions thereof are officially free of bovine brucellosis (2009/600/ EC), 2009.
 
3.
Szulowski K, Pilaszek J, Iwaniak W. Application of meat juice in diagnosis of brucellosis in hares and wild boars by ELISA. Bull Vet Inst Pulawy. 2000; 44(1): 45–52.
 
4.
Pilaszek J, Szulowski K, Iwaniak W. Epidemiological condition of animal brucellosis in Poland. Medycyna Wet. 2000; 56(6): 363–366 (in Polish).
 
5.
Szulowski K, Iwaniak W, Pilaszek J, Murat J. Wild boars and hares as reservoirs of Brucella suis biovar 2 in Poland. Proceeding of the Brucellosis 2008 International Research Conference (Including the 61 st Brucellosis Research Conference); Sep 10 – 13 2008, Royal Holloway College, University of London, UK, p. 137.
 
6.
OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, Office International des Epizooties, Paris, France, 2009.
 
7.
Commission Regulation (EC) No 535/2002 of 21 March 2002 amending Annex C to Council Directive 64/432/EEC and amending Decision 2000/330/EC, 2002.
 
8.
Wiśniowski J, Królak M, Drożdżyńska M. Standard technique of anti-globulin test in diagnosis of bovine brucellosis. NVRI Puławy, Poland, 1978 (in Polish).
 
9.
Królak M, Stryszak M. Standard technique of 2-mercaptoethanol test in diagnosis of animal brucellosis. NVRI Puławy, Poland, 1979 (in Polish).
 
10.
Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the brucellosis laboratory. Institut National de la Recherche Agronomique, Paris, France. 1988.
 
11.
Lopez-Goni I, Garcia-Yoldi D, Martin CM, Miguel MJ, Munoz PM, Blasco JM, Jacques I, Grayon M, Cloeckeart A, Ferreira AC, Carloso R, Correa de Sa, MI, Walravens K, Albert D, Garin-Bastuji B. Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species including the vaccine strains. J Clin Microbiol. 2008; 46(10): 3484–3487.
 
12.
Le Fleche P, Jacques I, Grayon M, Al Dahouk S, Bouchon P, Denoeud F, Nockler K, Neubauer H, Guilloteau LA, Vergnaud G. Evaluating and selection of tandem repeat loci for Brucella MLVA typing assay. BMC Microbiol. 2006; 6(9): 1–14.
 
13.
Godfroid J, Cloeckaert A, Liautard JP, Kohler S, Fretin D, Walravens K, Garin-Bastuji B, Letesson JJ. From the discovery of the Malta fever s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been re-emerging zoonosis. Vet Res. 2005; 36(3): 313–326.
 
14.
Sixth Report. Joint FAO/WHO Expert Committee on Brucellosis. World Health Organization, Geneva, Switzerland, 1986.
 
15.
Johnson RP, Boag L, Anderson S, Holtslander R, Rahn K, Clarke RC, Renwick SA, Alves D, Wilson JB, Spika J. Brucella abortus serology in cattle naturally infected with Escherichia coli O:157.H7. Vet Rec. 1994; 135(16): 382–383.
 
16.
Weynants V, Tibor A, Denoel P, Saegerman C, Godfroid J, Thiange P, Letesson JJ. Infection of cattle with Yersinia enterocolitica O:9, a cause of false positive serological reactions in bovine brucellosis diagnostic tests. Vet Microbiol. 1996; 48(1–2): 101–112.
 
17.
Gerbier G, Garin-Bastuji B, Pouillot R, Very P, Cau C, Berr V, Dufour B, Moutou F. False positive serological reactions in bovine brucellosis: evidence of the role of Yersinia enterocolitica serotype O:9 in a field trial. Vet Res. 1997; 28(4): 375–383.
 
18.
Pouillot R, Lescoat P, Garin-Bastuji B, Repiquet D, Terrier P, Gerbier G, Bénet JJ, Sanaa M. Risk factors for false-positive serological reactions for bovine brucellosis in Saône-et-Loire (France). Prev Vet Med. 1998; 35(3): 165–179.
 
19.
Godfroid J, Käsbohrer A. Brucellosis in the European Union and Norway at the turn of the twenty-first century. Vet Microbiol. 2002; 90(1–4): 135–145.
 
20.
Muñoz PM, Marín CM, Monreal D, González D, Garin-Bastuji B, Díaz R, Mainar-Jaime RC, Moriyón I, Blasco JM. Efficacy of Several Serological Tests and Antigens for Diagnosis of Bovine Brucellosis in the Presence of False-Positive Serological Results Due to Yersinia enterocolitica O:9. Clin Diagn Lab Immunol. 2005; 12(1): 141–151.
 
21.
Weiner M, Złotnicka J, Iwaniak W, Szulowski, K. Development of a multiplex PCR for identification of Brucella sp. and cross-reacting Yersinia enterocolitica O:9. Bull Vet Inst Pulawy. 2011; 55(4): 603–607.
 
22.
Caroff M, Bundle DR, Perry MB. Structure of the phenol-phase soluble cellular lipopoly saccharide of Yersinia enterocolitica serotype O:9. Eur J Biochem. 1984; 139(1): 195–200.
 
23.
Kittelberger R, Hilbink F, Hansen MF, Penrose M, De Lisle GW, Letesson JJ, Garin-Bastuji B, Searson J, Fossati CA, Cloeckaert A, Schurig G. Serological crossreactivity between Brucella abortus and Yersinia enterocolitica O:9. I Immunoblot analysis of the antibody response to Brucella protein antigens in bovine brucellosis. Vet Microbiol. 1995; 47(3–4): 257–270.
 
24.
Szulowski K, Iwaniak W, Weiner M, Złotnicka J, Szymajda M, Zaręba Z, Czępińska H. Diagnosis and epidemiological condition of bovine brucellosis in Poland. Medycyna Wet. 2012; 68(2): 110–113 (in Polish).
 
25.
Cook DR, Noble JW. Isolation of Brucella suis from cattle. Aus Vet J. 1984; 61(8): 263–264.
 
26.
Corbel MJ. Brucellosis: an overview. Emerg Infect Dis. 1997; 3(2): 213–221.
 
27.
Ewalt DR, Payeur JP, Rhyan JC, Geer PL. Brucella suis biovar 1 in naturally infected cattle: a bacteriological, serological and histological study. J Vet Diagn Invest. 1997; 9(4): 417–420.
 
28.
Olsen SC, Hennager SG. Immune response and Protection against Experimental Brucella suis Biovar 1 Challenge in Nonvaccinated or B. abortus Strain RB51-Vaccinated Cattle. Clin Vaccine Immunol. 2010; 17(12): 1891–1895.
 
29.
Luna-Martinez JE, Mejia-Teran C. Brucellosis in Mexico: current status and trends. Vet Microbiol. 2002; 90(1–4): 19–30.
 
30.
Garcia Carillo C. Animal and human brucellosis in the Americas. OIE, Paris, 1990.
 
31.
Samartino LE. Brucellosis in Argentina. Vet Microbiol. 2002; 90(1–4): 71–80.
 
32.
Lucero NE, Ayala SM, Escobar GI, Jacob NR. Brucella isolated in humans and animals in Latin America from 1968 to 2006. Epidemiol Infect. 2008; 136(4): 496–503.
 
33.
Andersen FM, Pedersen KB. Brucellosis: a case of natural infection of a cow with Brucella suis biotype 2. Dan Vet. 1995; 78(8): 408.
 
34.
Cvetnic Z, Mitak M, Ocepek O, Lojkic M, Terzic S, Jemersic L, Humski A, Habrun B, Sostaric B, Brstilo M, Krt B, Garin-Bastuji B. Wild boars (Sus scrofa) as reservoirs of Brucella suis biovar 2 in Croatia. Acta Vet Hung 2003; 51(4): 465–473.
 
35.
Melzer F, Lohse R, Nieper H, Liebert M, Sachse K. A serological study on brucellosis in wild boars in Germany. Eur J Wildl Res. 2007; 53(2): 153–157.
 
36.
Gyuranecz M, Erdélyi K, Makrai L, Fodor L, Szépe B, Ráczné Mészáros A, Dán A, Dencső L E, Fassang E, Szeredi L. Brucellosis of the European Brown Hare (Lepus europaeus). J Comp Path. 2011; 145(1): 1–5.
 
37.
Królak M, Owadiuk Z. Immunogenic and diagnostic values of vaccine Duphavac in eradication of bovine brucellosis. Życie Wet. 1994; 10: 375–379 (in Polish).
 
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