Introduction and objective:
The study assesses the levels of urinary pyrethroid pesticide (PYR) in women during early pregnancy. The factors associated with exposure are also determined.

Material and methods:
A total of 480 pregnant women from non-rural areas visiting hospital for prenatal examination during early pregnancy were enrolled. A self-designed, structured questionnaire was used to collect data on potential factors of PYR exposure. Urinary PYR metabolite levels were quantified using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS).

The majority of urine samples (98.8%) contained one or more PYR metabolite, although only a few women self-reported pesticide exposure. Urinary 3-phenoxybenzoic acid (3PBA) levels were close to those reported in certain developed countries. However, the levels of 3-(2,2-dibromovinyl)-2,2-dimethylcyclopropane-1-carboxylic acid (DBCA) and 4-fluoro-3-phenoxybenzoic acid (4F3PBA) were higher than those reported in previous studies. Urinary PYR levels were positively associated with exposure to pesticides, consumption of bananas and oranges, the number of fruit types the women regularly ate, being multiparous, and cooked frequently. They were negatively associated with early pregnancy body mass index (BMI), unemployment, frequent intake of apples, and washing fruits and vegetables with soda or hot water.

Pregnant women in non-rural areas were extensively exposed to low levels of PYRs. Dietary intake may be the primary pathway of exposure. The presented findings highlight the importance of using appropriate methods to reduce pesticide residues in food.

The study was carried out in the School of Pharmaceutical Sciences and Yunnan Provincial Key Laboratory of Pharmacology for Natural Products, Kunming Medical University. This work was supported by the National Natural Science Foundation of China, 81673186, and Yunnan Provincial Collaborative Innovation Centre for Public Health and Disease Prevention and Control, Grant No. 2015YNPHXT01.
Huang YF, Pan WC, Tsai YA, et al. Concurrent exposures to nonylphenol, bisphenol A, phthalates, and organophosphate pesticides on birth outcomes: A cohort study in Taipei, Taiwan. Sci Total Environ. 2017; 607–608: 1126–1135.
Arbuckle TE, Lin Z, Mery LS. An exploratory analysis of the effect of pesticide exposure on the risk of spontaneous abortion in an Ontario farm population. Environ Health Perspect. 2001; 109(8): 851–7.
Jaacks LM, Diao N, Calafat AM, et al. Association of prenatal pesticide exposures with adverse pregnancy outcomes and stunting in rural Bangladesh. Environ Int. 2019; 133(Pt B): 105243.
Bouchard MF, Chevrier J, Harley KG, et al. Prenatal exposure to organophosphate pesticides and IQ in 7-year-old children. Environ Health Perspect. 2011; 119(8): 1189–95.
Koureas M, Tsakalof A, Tsatsakis A, et al. Systematic review of biomonitoring studies to determine the association between exposure to organophosphorus and pyrethroid insecticides and human health outcomes. Toxicol Lett. 2012; 210(2): 155–68.
Rowe C, Gunier R, Bradman A, et al. Residential proximity to organophosphate and carbamate pesticide use during pregnancy, poverty during childhood, and cognitive functioning in 10-year-old children. Environ Res. 2016; 150: 128–37.
Van Maele-Fabry G, Gamet-Payrastre L, and Lison D. Household exposure to pesticides and risk of leukemia in children and adolescents: Updated systematic review and meta-analysis. Int J Hyg Environ Health. 2018.
Chevrier C, Beranger R. Pesticides and Child’s Health in France. Curr Environ Health Rep. 2018.
Smith PA, Thompson MJ, Edwards JW. Estimating occupational exposure to the pyrethroid termiticide bifenthrin by measuring metabolites in urine. J Chromatogr B Analyt Technol Biomed Life Sci. 2002; 778(1–2): 113–20.
Fortes C, Mastroeni S, Pilla MA, et al. The relation between dietary habits and urinary levels of 3-phenoxybenzoic acid, a pyrethroid metabolite. Food Chem Toxicol. 2013; 52: 91–6.
Morgan MK. Children’s exposures to pyrethroid insecticides at home: a review of data collected in published exposure measurement studies conducted in the United States. Int J Environ Res Public Health. 2012; 9(8): 2964–85.
Glorennec P, Serrano T, Fravallo M, et al. Determinants of children’s exposure to pyrethroid insecticides in western France. Environ Int. 2017; 104: 76–82.
Le Grand R, Dulaurent S, Gaulier JM, et al. Simultaneous determination of five synthetic pyrethroid metabolites in urine by liquid chromatography-tandem mass spectrometry: application to 39 persons without known exposure to pyrethroids. Toxicol Lett. 2012; 210(2): 248–53.
Furlong MA, Barr DB, Wolff MS, et al. Prenatal exposure to pyrethroid pesticides and childhood behavior and executive functioning. Neurotoxicology. 2017; 62: 231–238.
Watkins DJ, Fortenberry GZ, Sanchez BN, et al. Urinary 3-phenoxybenzoic acid (3-PBA) levels among pregnant women in Mexico City: Distribution and relationships with child neurodevelopment. Environ Res. 2016; 147: 307–13.
Bempelou E, Kappatos P, and Liapis K. Determination of Eight Sulfonylurea Herbicide Residues by LC/MS/MS Using a SampleSeparation Technique with Ethyl Acetate. J AOAC Int. 2018.
Lewis RC, Cantonwine DE, Anzalota Del Toro LV, et al. Urinary biomarkers of exposure to insecticides, herbicides, and one insect repellent among pregnant women in Puerto Rico. Environ Health. 2014; 13: 97.
Dereumeaux C, Saoudi A, Goria S, et al. Urinary levels of pyrethroid pesticides and determinants in pregnant French women from the Elfe cohort. Environ Int. 2018; 119: 89–99.
Berkowitz GS, Obel J, Deych E, et al. Exposure to indoor pesticides during pregnancy in a multiethnic, urban cohort. Environ Health Perspect. 2003; 111(1): 79–84.
Ding G, Cui C, Chen L, et al. Prenatal exposure to pyrethroid insecticides and birth outcomes in Rural Northern China. J Expo Sci Environ Epidemiol. 2015; 25(3): 264–70.
Qi X, Zheng M, Wu C, et al. Urinary pyrethroid metabolites among pregnant women in an agricultural area of the Province of Jiangsu, China. Int J Hyg Environ Health. 2012; 215(5): 487–95.
Castorina R, Bradman A, Fenster L, et al. Comparison of current-use pesticide and other toxicant urinary metabolite levels among pregnant women in the CHAMACOS cohort and NHANES. Environ Health Perspect. 2010; 118(6): 856–63.
Barr DB, Olsson AO, Wong LY, et al. Urinary concentrations of metabolites of pyrethroid insecticides in the general U.S. population: National Health and Nutrition Examination Survey 1999–2002. Environ Health Perspect. 2010; 118(6): 742–8.
Trošt K, Ulaszewska MM, Stanstrup J, et al. Host: Microbiome co-metabolic processing of dietary polyphenols – An acute, single blinded, cross-over study with different doses of apple polyphenols in healthy subjects. Food Res Int. 2018; 112: 108–128.
Koutsos A, Lima M, Conterno L, et al. Effects of Commercial Apple Varieties on Human Gut Microbiota Composition and Metabolic Output Using an In Vitro Colonic Model. Nutrients. 2017; 9(6).
Yang T, Doherty J, Zhao B, et al. Effectiveness of Commercial and Homemade Washing Agents in Removing Pesticide Residues on and in Apples. J Agric Food Chem. 2017; 65(44): 9744–9752.
Wu Y, An Q, Li D, et al. Comparison of Different Home/Commercial Washing Strategies for Ten Typical Pesticide Residue Removal Effects in Kumquat, Spinach and Cucumber. Int J Environ Res Public Health. 2019; 16(3).
Deanovic LA, Stillway M, Hammock BG, et al. Tracking pyrethroid toxicity in surface water samples: Exposure dynamics and toxicity identification tools for laboratory tests with Hyalella azteca (Amphipoda). Environ Toxicol Chem. 2018; 37(2): 462–472.
Julien R, Adamkiewicz G, Levy JI, et al. Pesticide loadings of select organophosphate and pyrethroid pesticides in urban public housing. J Expo Sci Environ Epidemiol. 2008; 18(2): 167–74.
Mattei C, Dupont J, Wortham H, et al. Influence of pesticide concentration on their heterogeneous atmospheric degradation by ozone. Chemosphere. 2019; 228: 75–82.
Mercier F, Gilles E, Saramito G, et al. A multi-residue method for the simultaneous analysis in indoor dust of several classes of semi-volatile organic compounds by pressurized liquid extraction and gas chromatography/tandem mass spectrometry. J Chromatogr A. 2014; 1336: 101–11.
Liu YL, Wang JZ, Peng SC, et al. [Modeling the Environmental Behaviors and Ecological Risks of Permethrin in Chaohu Lake]. Huan Jing Ke Xue. 2016; 37(12): 4644–4650.
Voerman E, Santos S, Inskip H, et al. Association of Gestational Weight Gain With Adverse Maternal and Infant Outcomes. JAMA. 2019; 321(17): 1702–1715.
Darney K, Bodin L, Bouchard M, et al. Aggregate exposure of the adult French population to pyrethroids. Toxicol Appl Pharmacol. 2018; 351: 21–31.
Heudorf U, Angerer J. Metabolites of pyrethroid insecticides in urine specimens: current exposure in an urban population in Germany. Environ Health Perspect. 2001; 109(3): 213–7.
Goen T, Schmidt L, Lichtensteiger W, et al. Efficiency control of dietary pesticide intake reduction by human biomonitoring. Int J Hyg Environ Health. 2017; 220(2 Pt A): 254–260.
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