Recurrence of Stachybotrys chartarum during mycological and toxicological study of bioaerosols collected in a dairy cattle shed
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Universite de Caen Basse Normandie, Caen, France
Laboratoire Departemental Frank Duncombe, Conseil General du Calvados, Saint Contest, France
Corresponding author
David Garon   

Universite de Caen Basse Normandie, Caen, France
Ann Agric Environ Med. 2012;19(1):61-67
Agricultural occupations associated with animal breeding and the processing of animal materials in confinement systems could potentially lead to bioaerosol exposures. Moulds and mycotoxins could be constituents of bioaerosols and should be studied because of their possible involvement in respiratory diseases and cancers. In order to characterize the fungal contamination of the indoor air in a dairy barn, bioaerosols were collected during 20 days in a cattle farm located in Normandy (France). Mycobiota, mycotoxins and the mutagenicity of bioaerosols were studied. The toxigenic ability of Aspergillus flavus group and Aspergillus fumigatus isolates was also evaluated in vitro. The prevalent airborne moulds were from the following potentially toxigenic species: Aspergillus flavus group, Aspergillus fumigatus, Penicillium chrysogenum, Stachybotrys chartarum, and the allergenic species Ulocladium chartarum, Cladosporium cladosporioides. In comparison with harvesting, grain handling or broiler breeding, the concentrations of viable moulds were lower in the cattle shed. Seasonal variations in levels of several species were also observed. This study revealed that aflatoxins were detected in bioaerosols and, for the first time, showed that farmers are possibly exposed to Stachybotrys chartarum during routine barn work. Moreover, the finding of mutagenicity from bioaerosols needs further investigations on bioaerosol composition.
AGRESTE (Statistiques Agricoles, Ministère de l’Agriculture et de la Pêche). http://www.agreste.agriculture... (access:2011.11.15).
Mitchell DC, Schenker MB. Protection against breathing dust: behavior over time in Californian farmers. J Agric Saf Health. 2008; 14(2): 189-203.
Douwes J, Thorne P, Pearce N, Heederik D. Bioaerosol health effects and exposure assessment: progress and prospects. Ann Occup Hyg. 2003; 47(3): 187-200.
Adhikari A, Reponen T, Lee SA, Grinshpun SA. Assessment of human exposure to airborne fungi in agricultural confinements: personal inhalable sampling versus stationary sampling. Ann Agric Environ Med. 2004; 11(2): 269-277.
Lanier C, Richard E, Heutte N, Picquet R, Bouchart V, Garon D. Airborne molds and mycotoxins associated with handling of corn silage and oilseed cakes in agricultural environment. Atmos Environ. 2010; 44: 1980-1986.
Sorensen WG, Jones W, Simpson J, Davidson JI. Aflatoxin in respirable airborne peanut dust. J Toxicol Environ Health. 1984; 14: 525-533.
Straumfors Halstensen A, Nordy KC, Sletner Klemsdal S, Elen O, Clasen PE, Eduard W. Toxigenic Fusarium spp. as determinant of trichothecene mycotoxins in settled grain dust. J Occup Environ Hyg. 2006; 3: 651-659.
Millner PD. Bioaerosols associated with animal production operations. Bioresour Technol. 2009; 100: 5379-5385.
Linaker C, Smedley J. Respiratory illness in agricultural workers. Occup Med (Lond). 2002; 52(8): 451-459.
Reboux G, Piarroux R, Mauny F, Madroszyk A, Millon L, Bardonnet K, Dalphin JC. Role of molds in farmer’s lung disease in Eastern France. Am J Respir Crit Care Med. 2001; 163: 1534-1539.
Larsson K, Malmberg P, Eklund A, Belin L, Blaschke E. Exposure to microorganisms, airway inflammatory changes and immune reactions in asymptomatic dairy farmers. Bronchoalveolar lavage evidence of macrophage activation and permeability changes in the airways. Int Arch Allergy Appl Immunol. 1988; 87(2): 127-133.
Poulsen OM, Breum NO, Ebbehøj N, Hansen AM, Ivens UI, van Lelieveld D, et al. Sorting and recycling of domestic waste. Review of occupational health problems and their possible causes. Sci Total Environ. 1995; 168(1): 33-56.
Vogelzang PF, van der Gulden JW, Folgering H, van Schayck CP. Organic dust toxic syndrome in swine confinement farming. Am J Ind Med. 1999; 35: 332-334.
Autrup JL, Schmidt J, Autrup H. Exposure to aflatoxin B 1 in animal-feed production plant workers. Environ Health Perspect. 1993; 99: 195-197.
Dvořáčková I. Aflatoxin inhalation and alveolar cell carcinoma. BMJ. 1976; 1: 691.
Hayes RB, van Nieuwenhuize JP, Raatgever JW, ten Kate FJ. Aflatoxin exposures in the industrial setting: an epidemiological study of mortality. Food Chem Toxicol. 1984; 22(1): 39-43.
Olsen JH, Dragsted L, Autrup H. Cancer risk and occupational exposure to aflatoxins in Denmark. Br J Cancer. 1988; 58: 392-396.
Richard JL. Some major mycotoxins and their mycotoxicoses-An overview. Int J Food Microbiol. 2007; 119(1-2): 3-10.
Wang JS, Groopman JD. DNA damage by mycotoxins. Mutat Res. 1999; 424(1-2): 167-181.
Klich MA. Identification of common Aspergillus species. Centraalbureau voor Schimmelcultures (Utrecht), 2002.
Booth C. The genus Fusarium. Commonwealth Mycological Institute (Kew), 1966.
Domsch KH, Gams W, Anderson TH. Compendium of soil fungi. Academic Press (London), 1980.
Pitt JI. The genus Penicillium and its teleomorphic states Eupenicillium and Talaromyces. Academic Press (London), 1979.
Samson RA, Frisvad JC. Penicillium subgenus Penicillium: new taxonomic schemes. mycotoxins and others extrolites. Centraalbureau voor Schimmelcultures (Utrecht), 2004.
Samson RA, Hoekstra ES, Frisvad JC, Filtenborg O. Introduction to food and airborne fungi. Centraalbureau voor Schimmelcultures (Utrecht), 2002.
Von Arx JA. The genus of fungi sporulating in pure culture. Cramer (Vaduz), 1981.
Garon D, El Kaddoumi A, Carayon A, Amiel C. FT-IR spectroscopy for rapid differentiation of Aspergillus flavus , Aspergillus fumigatus, Aspergillus parasiticus and characterization of aflatoxigenic isolates collected from agricultural environment. Mycopathologia. 2010; 170: 131-142.
Mortelmans K, Zeiger E. The Ames Salmonella/microsome mutagenicity assay. Mutat Res. 2000; 455(1-2): 29-60.
Hanhela R, Louhelainen K, Pasanen AL. Prevalence of microfungi in Finnish cow barns and some aspects of the occurrence of Wallemia sebi and Fusaria. Scand J Work Environ Health. 1995; 21(3): 223-228.
Campbell AR, Swanson MC, Fernandez-Caldas E, Reed CE, May JJ, Pratt DS. Aeroallergens in dairy barns near Cooperstown, New York and Rochester, Minnesota. Am Rev Respir Dis. 1989; 140(2): 317-320.
Pasanen AL, Kalliokoski P, Pasanen P, Salmi T, Tossavainen A. Fungi carried from farmers’ work into farm homes. Am Ind Hyg Assoc J. 1989; 50(12): 631-633.
Trout D, Bernstein J, Martinez K, Biagini R, Wallingford K. Bioaerosol lung damage in a worker with repeated exposure to fungi in a water-damaged building. Environ Health Perspect. 2001; 109(6): 641-644.
Hossain MA, Ahmed MS, Ghannoum MA. Attributes of Stachybotrys chartarum and its association with human disease. Allergy Clin Immunol. 2004; 113(2): 200-208.
Harrach B, Bata A, Bajmócy E, Benko M. Isolation of satratoxins from the bedding straw of a sheep flock with fatal stachybotryotoxicosis. Appl Environ Microbiol. 1983; 45(5): 1419-1422.
Piecková E, Jesenská Z. Microscopic fungi in dwellings and their health implications in humans. Ann Agric Environ Med. 1999; 6(1): 1-11.
Lis DO, Mainelis G, Gorny RL. Microbial air contamination in farmhouses - quantitative aspects. Clean. 2008; 36(7): 551-555.
Seedorf J, Hartunga J, Schrödera M, Linkerta KH, PhillipsVR, Holden MR, et al. Concentrations and Emissions of Airborne Endotoxins and Microorganisms in Livestock Buildings in Northern Europe. J Agric Engng Res. 1998; 70: 97-109.
Kullman GJ, Thorne PS, Waldron PF, Marx JJ, Ault B, Lewis DM, et al. Organic dust exposures from work in dairy barns. Am Ind Hyg Assoc J. 1998; 59(6): 403-413.
Adhikari A, Sen MM, Gupta-Bhattacharya S, Chanda S. Volumetric assessment of airborne fungi in two sections of a rural indoor dairy cattle shed. Environ Int. 2004; 29(8): 1071-1078.
Abd-Elall AMM, Mohamed MEM, Awadallah MAI. Potential airborne microbial hazards for workers on dairy and beef cattle farms in Egypt. Vet Ital. 2009; 45(2): 275-285.
Lange JL, Thorne PS, Kullman GJ. Determinants of culturable bioaerosol concentrations in dairy barns. Ann Agric Environ Med. 1997; 4: 187-194.
Takai H, Pedersen S, Johnsen JO, Metz JHM, Groot Koerkamp PWG, Uenk GH, et al. Concentrations and Emissions of Airborne Dust in Livestock Buildings in Northern Europe. J Agric Engng Res. 1998; 70: 59-77.
Radon K, Danuser B, Iversen M, Monso E, Weber C, Hartung J, et al. Air contaminants in different European farming environments. Ann Agric Environ Med. 2002; 9(1): 41-48.
Nardoni S, Mancianti F, Sgorbini M, Taccini F, Corazza M. Identification and seasonal distribution of airborne fungi in three horse stables in Italy. Mycopathologia. 2005; 160(1): 29-34.
Lee SA, Adhikari A, Grinshpun SA, McKay R, Shukla R, Reponen T. Personal exposure to airborne dust and microorganisms in agricultural environments. J Occup Environ Hyg. 2006; 3(3): 118-130.
Hill RA, Wilson DM, Burg WR, Shotwell OL. Viable fungi in corn dust. Appl Environ Microbiol. 1984; 47(1): 84-87.
Swan JRM, Crook B. Airborne microorganisms associated with grain handling. Ann Agric Environ Med. 1998; 5: 7-15.
Sutton P, Waring P, Müllbacher A. Exacerbation of invasive aspergillosis by the immunosuppressive fungal metabolite, gliotoxin. Immunol Cell Biol. 1996; 74(4): 318-322.
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