RESEARCH PAPER
Prevalence of Coxiella burnetii infection in foresters in the south-eastern Poland and comparison of diagnostic methods
| 1 | Laboratory of Serological Diagnosis/Cattle and Sheep Disease, National Veterinary Research Institute, Pulawy, Poland |
| 2 | Department of Allergology and Environmental Hazards, Institute of Rural Health, Lublin, Poland |
| 3 | Department of Food Safety and Veterinary , Ministry of Agriculture and Rural Development, Warsaw, Poland |
Ann Agric Environ Med. 2013;20(4):699–704
KEYWORDS
ABSTRACT
Introduction:
Coxiella burnetii is an aetiologic agent of Q fever. Ticks have been considered as a reservoir and a vector of C. burnetii. The outbreaks of Q fever were noted in different parts of Europe both in animals and humans. Q fever is zoonotic diseases characterized by sudden fever, headache, atypical pneumonia and abortions. Aim: The aim of the study was to investigate the presence of C. burnetii infection in foresters in the south–eastern endemic region of Poland and comparison of diagnostic methods.
Material and Methods:
The serum samples were collected from 304 humans (foresters) from the 12 superintendency in the south-eastern Poland. Additionally, 1,200 ticks Ixodes ricinus were collected from the same area. The sera samples were tested by using serological methods (CFT, ELISA and IFA) and the ticks were tested with the use of real-time PCR.
Results:
The results demonstrates that infections with C. burnetii are present in foresters in the south-eastern Poland. The serological results showed both chronic and acute form of Q fever. The statistical analysis of the positive results obtained by three different serological methods revealed that values of χ2 were statistically significant between IFA and ELISA and CFT and IFA. The final percentage of examined foresters classified as positive was 10.52%. No significant differences were observed between CFT and ELISA (p=0.25).The percentage of infected ticks was 15.9%.
Conclusions:
The study demonstrates that infections with C. burnetii are present in foresters in the south-eastern Poland. The comparison of serological methods showed that their diagnostic potential is differentiated but the most useful tool is IFA.
Coxiella burnetii is an aetiologic agent of Q fever. Ticks have been considered as a reservoir and a vector of C. burnetii. The outbreaks of Q fever were noted in different parts of Europe both in animals and humans. Q fever is zoonotic diseases characterized by sudden fever, headache, atypical pneumonia and abortions. Aim: The aim of the study was to investigate the presence of C. burnetii infection in foresters in the south–eastern endemic region of Poland and comparison of diagnostic methods.
Material and Methods:
The serum samples were collected from 304 humans (foresters) from the 12 superintendency in the south-eastern Poland. Additionally, 1,200 ticks Ixodes ricinus were collected from the same area. The sera samples were tested by using serological methods (CFT, ELISA and IFA) and the ticks were tested with the use of real-time PCR.
Results:
The results demonstrates that infections with C. burnetii are present in foresters in the south-eastern Poland. The serological results showed both chronic and acute form of Q fever. The statistical analysis of the positive results obtained by three different serological methods revealed that values of χ2 were statistically significant between IFA and ELISA and CFT and IFA. The final percentage of examined foresters classified as positive was 10.52%. No significant differences were observed between CFT and ELISA (p=0.25).The percentage of infected ticks was 15.9%.
Conclusions:
The study demonstrates that infections with C. burnetii are present in foresters in the south-eastern Poland. The comparison of serological methods showed that their diagnostic potential is differentiated but the most useful tool is IFA.
REFERENCES (30)
2.
Georgiev M, Afonso A, Neubauer H, Needham H, Thiéry R, Rodolakis A, et al. Q fever in humans and farm animals in four European countries, 1982 to 2010. EuroSurveill 2013;18:pii=20407. Available at line: http://www.eurosurveillance.or....
3.
Lutyński R. First focus of Q fever on the territority of Poland. Przegl Lek. 1956; 12: 187–188.
4.
Bielawska-Drózd A, Cieślik P, Mirski T, Bartoszcze M, Knap J P, Gaweł J, et al. Q fever-selected issues. Ann Agric Environ Med. 2013; 20: 222–232.
5.
Niemczuk K, Szymańska-Czerwińska M, Zarzecka A, Konarska H. Q fever in a cattle herd and humans in the south-eastern Poland. Laboratory diagnosis of the diseases using serological and molecular methods. Bull Vet Inst Pulawy 2011; 55: 593–598.
6.
Herremans T, Hogeman B M, Naubuurs M, Peeters M, WegdamBlans M, Schneeberger P, et al. Comparison of the performance of IFA, CFT and ELISA assays for the serodiagnosis of acute Q fever by quality assessment. Diagn Microbiol Infect Dis. 2013; 75: 16–21.
7.
Panning M, Kilwinski J, Greiner-Fischer S, Peters M, Kramme S, Frangoulidis D, et al High throughput system and automated DNA preparation. BMC Microbiol 2008, 8, dois:10.1186/I471-2180-8-77.
8.
Wielders CCH, Kampschreur LM, Schneeberger PM, Jager MM, Hoepelman AIM, Leenders ACAP, et al. Early diagnosis and treatment of patient with symptomatic acute Q fever do not prohibit IgG antibody responses to Coxiella burnetii. Clin Vaccine Immunol. 2012; 19: 1661– 1666.
9.
Sidi-Boumedine K, Rousset E, Henning K, Ziller M. Development of harmonised schemes for the monitoring and reporting of Q fever in animals in the European Union. EFSA Report. 2010: 1–48.
10.
Chmielewski T, Tylewska-Wierzbanowska S. Q fever at the turn of the century. Pol J Microbiol. 2012; 61: 81–93.
11.
Galińska EM, Knap JP, Chmielewska-Badora J, 2011: Wstępne wyniki badań seroepidemiologicznych i klinicznych w kierunku gorączki Q u osób zawodowo narażonych. Med Ogólna. 2011; 17: 001–006.
12.
Chmielewski T, Sidi-Boumedine K, Duquesne V, Podsiadły E, Thiéry R, Tylewska-Wierzbanowska S. Molecular epidemiology of Q fever in Poland. Pol J Vet. 2009; 58: 9–13.
13.
Mikołajczyk E, Lewińska Z, Łojewska R, Rumin W, Kruszewska D. Serological reactions in humans during the outbreak of Q fever. Przegl Epidemiol. 1986; 40: 342–348.
15.
Cygan Z, Buczek J, Modzelewska A, Guzik Z. Q fever diagnosed serologically in herd of dairy cows. Vet Med. 1983; 39: 536–538.
16.
Knap J. The clinic of Q fever in humans and animals.: In: Anusz Z. Q fever in humans and animals, ART Olsztyn 1995.
17.
Cisak E, Chmielewska-Badora J, Mackiewicz B, Dutkiewicz J. Prevalence of antibodies to Coxiella burnetii among farming population in eastern Poland. Ann Agric Environ Med. 2003; 10: 265–267.
18.
Landais C, Fenollar F, Thuny F, Raoult D. From acute Q fever to endocarditis: serological follow-up strategy. Clin Infect Dis. 2007; 44: 1337–13340.
19.
Vranakis I, Kokkini S, Chochlakis D, Sandalakis V, Pasparaki E, Minadakis G, et al. Serological survey of Q fever in Crete, southern Greece. Comp Immunol Microbial Infect Dis. 2012; 35: 123–127.
20.
Sprong H, Tijsse-Klasen E, Langelaar M, De Bruni A, Fonvill M, Grassner F, et al. Prevalence of Coxiella burnetii in ticks after a large outbreaks of Q fever. Zoonoses Public Health 2012; 59: 69–75.
21.
Rehn F, Radvan R. Isolation of Coxiella burnetii from the tick Ixodes ricinus. Cesk Epidemiol Mikrobiol Imunol. 1957; 6: 85–88.
22.
Rehacek J, Urvolgyi J, Kocianova E, Sekeyova Z, Vavrekova M, Kovacova M. Extensive examination of different tick species for infestation with Coxiella burnetii in Slovakia. Eur J Epidemiol. 1991; 7: 299–303.
23.
Spitalska E, Kocianova E. Detection of Coxiella burnetii in ticks collected in Slovakia and Hungary. Eur J Epidemiol. 2003; 18: 263–266.
24.
Smetanova K, Schwarzova K, Kocianova E. Detection of Anaplasma phagocytophilum, Coxiella burnetii, Rickettsia spp., and Borrelia burgdorferi s. l. in ticks, and wild-living animals in western and middle Slovakia. Ann N Y Acad. 2006; 1078: 312–315.
25.
Barandika JF, Hurtado A, Garcia-Sanmartin J, Juste RA, Anda P, Garcia-Perez AL. Prevalence of tick-borne zoonotic bacteria in questing adult ticks from northern Spain. Vector Borne Zoonotic Dis. 2008; 6: 829–835.
26.
Astobiza I, Barral M., Ruiz-Fons F, Barandika JF, Gerrikagoitia X, Hurtado A, et al. Molecular investigation of the occurrence of Coxiella burnetii in wildlife and ticks in an endemic area. Vet Microbiol. 2011; 147: 190–194.
27.
Reye AL, Hubschen JM, Sausy A, Muller CP. Prevalence and seasonality of tick-borne pathogens in questing Ixodes ricinus ticks from Luxembourg. Appl Environ Microbiol. 2010; 76: 2923–2931.
28.
Rehacek J, Krauss H, Kocianova E, Kovacova E, Hinterberger G, Hanak P, et al. Studies of the prevalence of Coxiella burnetii, the agent of Q fever, in the foothills of the southern Bavarian Forest, Germany. Zentralbl Bakteriol. 1993; 278: 132–138.
29.
Hildebrandt A, Straube E, Neubauer H, and Schmoock G. Coxiella burnetii and coinfections in Ixodes ricinus ticks in central Germany. Vector Borne Zoonotic Dis. 2011; 11: 1205–1207.
30.
Anderson A, Bijlmer H, Fournier P E, Graves S, Hartzell J, Kersh G J, et al. Diagnosis and management of Q fever – United States, 2013. Recommendations from CDC and the Q fever Working Group MMWR 2013; 62: 1–29.
Improvement of visual identification of the journal - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher Education allocated to the activities of disseminating science.
Generation of the DOI (Digital Object Identifier) - task financed under the agreement No. 618/P-DUN/2019 by the Minister of Science and Higher
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