Increased urinary excretion of thioethers as a marker for detecting exposure to herbicide containing 2,4-dichlorophenoxyacetic acid dimethylamine - experimental study on mice.
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Institute of Occupational Health, Novi Sad, FR Yugoslavia
Institute of Hygiene and Medical Ecology, Belgrade, FR Yugoslavia
Department for Pharmacology, Toxicology and Clinical Pharmacology, Medical Faculty, Novi Sad, FR Yugoslavia
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Ivan Mikov   

Institute of Occupational Health, Novi Sad, FR Yugoslavia
Ann Agric Environ Med. 2000;7(1):61-63
The possibility that urinary thioethers concentration might be a marker for detecting exposure to herbicide containing 2,4-dichlorophenoxyacetic acid dimethylamine (2,4-DMA) was investigated in animals. Mice were treated with the herbicide containing 2,4-DMA consecutively for 4 days. Urinary concentrations of thioethers related either to body weight or creatinine concentration in urine in the group of animals treated with herbicide were significantly higher compared to control group. Results suggest that thioethers determination in urine might be a noninvasive and simple method for detecting exposure to herbicide containing 2,4-DMA.
Aldkofer F, Scherer G, Von Maltzan C, Von Meyerinck L, Jarczyk L, Martin F, Grimmer G: Dietary influences on urinary excretion of hydroxyphenanthrenes, thioethers and mutagenicity in man. IARC SCI Publ 1990, 104, 415-420.
Aringer L, Lidums V: Influence of diet and other factors on urinary levels of thioethers. Int Arch Environ Health 1988, 61, 123-130.
Bos RP, Van Popel G, Theuws JLG, Kok FJ: Decreased excretion thioethers in urine of smokers after use of -carotene. Int Arch Occup Environ Health 1992, 64, 189-193.
Dehnen W: A study on urinary thioethers by detecting N-acetylcysteine and thiophenol after alkaline hydrolysis. Zentralbl Hyg Umweltmed 1990, 189, 441-451.
El Gazzar RM, Abdel-Hamid H, Shamy MY: Biological monitoring of occupational exposure to electrophilic compounds. J Environ Pathol Toxicol Oncol 1994, 13, 19-23.
Ellman GL: Tissue sulfhydryl groups. Arch Biochem Biophys 1959, 82, 70-77.
Ferreira M Jr., Buchet JP, Burrion JB, Moro J, Cupers L, Delavignette JP, Jacques J, Lauwerys R: Determinants of urinary thioethers, D-glucaric acid and mutagenicity after exposure to polycyclic aromatic hydrocarbons assessed by air monitoring and measurement of 1-hydroxypyrene in urine: a cross-sectional study in workers of coke and graphite-electrode-producing plants. Int Arch Occup Environ Health 1994, 65, 329-338.
Griffin RJ, Salemme J, Clark J, Myers P, Burka LT: Billiary elimination of oral 2,4-dichlorphenoxyacetic acid and its metabolites in male and female Sprague-Dawley rats, B6C3F1 mice and Syrian hamsters. J Toxicol Environ Health 1997, 51, 401-413.
Henderson PT, Doorn RV, Leijdekkers CM, Bos RP: Excretion of thioethers after exposure to electrophilic chemicals. IARC Sci Publ 1984, 59, 173-187.
Ibrahim MA, Bond GG, Burke TA, Cole P, Dost FN, Enterline PE, Gough M, Greenberg RS, Halperin WE, McConnell E, Munro IC, Swenberg JA, Zahm SH, Graham JD: Weight of the evidence on the. human carcinogenicity of 2,4-D. Environmental Health Perspectives 1991, 96, 213-222.
Jovanovic S, Markovic O, Stanulovic M: Handbook of Clinical Biochemistry. Medical Society of Vojvodina, Novi Sad 1981 (in Serbian).
Knopp D: Assessment of exposure to 2,4-dichlorophenoxyacetic acid in the chemical industry: results of a five year biological monitoring study. Occup Environ Med 1994, 51(3), 152-159.
Knopp D, Glass S: Biological monitoring of 2,4-dichlorophenoxyacetic acid exposed workers in agriculture and forestry. Int Arch Occup Environ Health 1991, 63, 329-333.
Mikov I, Mikov M: State and problems of health protection in connection with the use of pesticides in agriculture of Vojvodina. Agric Med Rur Health 1996, 20, 19-22.
Mikov I: Evaluation of Liver Function Tests in Agricultural Workers Exposed to Herbicide 2,4-dichlorophenoxyacetic Acid. Doctoral thesis. University of Belgrade, Belgrade 1999 (in Serbian).
Monarca S, Scassellati-Sforzolini G, Donato F, Angeli G, Spiegelhalider B, Fatigoni C, Pasquini R: Biological monitoring of workers exposed to N-nitrosodiethanolamine in the metal industry. Environ Health Perspect 1996, 104, 78-82.
Palmeira CM, Moreno AJ, Madeira VM: Metabolic alterations in hepatocytes promoted by the herbicides paraquat, dinoseb and 2,4 D. Arch Toxicol 1994, 68, 24-31.
Palmeira CM, Moreno AJ, Madeira MC: Thiols metabolism is altered by the herbicides paraquat, dinoseb and 2,4 D: A study in isolated hepatocytes. Toxicol Lett 1995, 81, 115-123.
Paulino CA, Guerra JL, Oliveira GH, Palermo-Neto J: Acute, subchronic and chronic 2,4-dichlorophenoxyacetic acid (2,4-D) intoxication in rats. Vet Human Toxicol 1996, 38, 348-352.
Prescott LF, Park J, Darrien I: Treatment of severe 2,4-D and mecoprop intoxication with alkaline diuresis. Br J Clin Pharmac 1979, 7, 111-116.
RTECS: Registry of toxic effects of chemical substances. National Institute for Occupational Safety and Health, Cincinnati, OH (CD-ROM version). Micromedex, Inc, Englewood, CO 1992.
Sauerhoff MW, Braun WH, Blau GE, LeBeau JE: The fate of 2,4 dichlorophenoxyacetic acid (2,4-D) following oral administration to man. Toxicol Appl Pharmacol 1976, 37, 136-137.
Sinues B, Rueda P, Benitez J, Saenz MA, Bernal ML, Lanuza J, Alda O, Tres A, Bartolome M. Thioether excretion, urinary mutagenicity, and metabolic phenotype in smokers. J Toxicol Environ Health 1994, 43, 327-338.
Summer KH, Rozman K, Carlston F, Greim H: Urinary excretion of mercapturic acid in chimpanzees and rats. Toxicol Appl Pharmacol 1979, 50, 207-212.
Vainio H, Matos E, Kogevinas M: Identification of occupational carcinogens. In: Pearce N, Matos E, Vainio H, Boffetta P, Kogevinas M (Eds.): Occupational Cancer in Developing Countries, 41-59. WHO, International Agency for Research on Cancer, Institute of Occupational Health in Finland, International Labour Office, Lyon 1994.
Van Welie RT, Van Dijck RG, Vermeulen NP, Van Sittert NJ: Mercapturic acids, protein adducts, and DNA adducts as biomarkers of electrophilic chemicals. Crit Rev Toxicol 1992, 22, 271-306.
WHO: 2,4-dichlorophenoxyacetic acid (2,4-D). Environmental Health Criteria 29, WHO, Geneva 1984.
Wigfield YY, Lacrois MD, Lanouette M, Gurprasad NP: Gas chromatographic determination of N-nitrosodialkanolamines in herbicide di or trialkanolamine formulations. J Assoc Off Anal Chem 1988, 71, 328-333.
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